Primary Research Paper: Cell & Molecular Biology What is Zwilch?

Questions for Research Article What is the significance of the research conducted? What were the questions asked, hypotheses proposed, or goal of the research? Describe the trends or other descriptive data they presented? Briefly describe the design of the experiment (e.g. variables, controls, etc.). What types of statistical analysis was performed on the data? See handout and website for updates on questions

Sections of an Article Title Abstract Intro Fig … fig legends… results… methods Discussion References Redundant? See electronic handout on website for more details

Tells what was accomplished in the paper Rephrase in your own words Title Tells what was accomplished in the paper Rephrase in your own words Socrative

Abstract System Molecules pertinent to the study Terms Techniques Hypothesis or Goal Socrative What is the system in this paper?

Hypothesis State the hypothesis as an “if this… then” statement Socrative

Hypothesis Goal: to identify other components (than ZW10/ROD) of the complex necessary for accurate chromosome segregation -or- If Zwilch is an important component of the chromosome segregation complex

Then… Immunoaffinity chromatography confirm Zwilch part of complex Mass spectroscopy confirm Zwilch part of complex Localize to kinetochore & kinetochore microtubules same as ZW10/ROD Mutation same mitotic phenotypes as zw10/rod Evolutionarily conserved (drosophila & human same coppt w/ ZW10/ROD and localized to kinetochores at prometaphase)

New questions: weak interaction w/ kinesin-like kinetochore component CENP-meta

Introduction Significance Socrative Is the significance anywhere else in the paper? State one reason the research is significant Socrative

Significance This study is significant because …

Significance cell division is critical to many essential processes of multicellular organisms: growth & development, reproduction important to have a basic understanding of the process and of the regulation to ensure accuracy: deformity, cancer new information may contribute to a better understanding of human health and disease

Significance cell division is critical to many essential processes of multicellular organisms: growth & development, reproduction important to have a basic understanding of the process and of the regulation to ensure accuracy: deformity, cancer new information may contribute to a better understanding of human health and disease this study can contribute to our understanding of molecular interactions essential for accurate cell division by identifying and describing novel components of the kinetochore apparatus Be sure that your description of the significance includes both broad and specific implications of the work

Figure Legends Type of info Type of assay or experiment Results or conclusion

…can show us trends and relationships. Figures …can show us trends and relationships.

Trends or Relationship Data: photos of protein gels sequence alignment diagram pictures of cells The relationships illustrated were: interaction w/ ZW10/ROD colocalization correlation of their activities Supporting evidence: Evolutionary relationship, based on sequence and similarity of localization & fxn in human cells

Other elements Design of experiment Funding Control(s) Variables/Treatments Funding