Real-time Concentration

Slides:

Advertisements

Similar presentations

© ChromaCon AG // // Convert batch to MCSGP // ver. May Contichrom ® case study: How to convert a batch chromatography process.

Advertisements

Single-use Quantasep Systems First Bench-top Single-Use flow- path Chrom System with gradient capability.

End-to-end continuous production of complex recombinant proteins Integration of perfusion cultivation and automated multi-step purification

Gas Chromatography Introduction 1.) Gas Chromatography

Gas Chromatography.

M. HAERTLEIN, INSTITUT MAX VON LAUE - PAUL LANGEVIN 3 rd D-Lab Meeting, 28 th April 2003 EMBL Heidelberg Optimisation of auxotrophic strains for expression.

UPSTREAM DEVELOPMENT OF HIGH CELL DENSITY, PERFUSION PROCESSES FOR CONTINUOUS MANUFACTURING Tim Johnson, Ph.D. October 21, 2013.

Protein purification in practice Quantification of the procedure –How well did it work? –Did something go wrong? Where? Know how much fumarase is present.

Ken McFarland Pascal Matengo. Lab Overview  Measure flow of water using three different flow meters  Measure flow manually to obtain actual flow rate.

Launching MIURA sample prep system

Nucleic Acid Prep Station What we can do for you!.

Isolation and Characterization of Cellular Complexes Containing the Histone Deacetylase SIRT1 Vincent Lew Howard Hughes Medical Institute Fellowship Mentor:

Protein Purification. Why purify Proteins? Characterize Function Activity Structure Study protein regulation and protein interactions Use in assays Produce.

Platform downstream processes in the age of continuous chromatography: A case study Mark Brower BioProcess Technology & Expression Bioprocess Development.

Tryptic digestion Proteomics Workflow for Gel-based and LC-coupled Mass Spectrometry Protein or peptide pre-fractionation is a prerequisite for the reduction.

Biochemistry February Lecture Analytical & Preparative Protein Chemistry II.

Magnet Analytical Chemistry Unit 4

High Performance Liquid Chromatography (HPLC) Jeannette Comeau CHEE April 2004.

By: Thilag.k & Stephen. What is Hpcl??? Hplc or high performance liquid chromatography is the most widely used analytical separation technique. The difference.

Protein Purification from Corn Germ Danielle McConnell Department of Chemical Engineering Iowa State University.

PRODUCTION OF HIGHLY PURIFIED BOVINE TRANSFERRIN Group 5B Arjun Goel, Connie Marcelli, Lianne Kark, Sara Dang, Vivien Tai Faculty Advisor Prof C.A. Mims.

Zensol Automation Inc. ZENSOL First Trip Monitoring In addition to doing timing and motion on circuit breakers, our CBA-32P instruments are capable of.

Large Molecule Technologies  Cell Culture/Fermentation Metabolite feedback control  In-Line Nova/NovaFlex/BGA  FTIR/NIR/Raman Capacitance (cell mass)

Lab 6 Salt fractionation In aqueous environments salt ions interact strongly with their complementary charge on a water molecule If the concentration of.

$100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300.

$100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300.

PROTEIN PURIFICATION AND ANALYSIS. Assays Need measures for the object (enzyme activity, chromophore, etc.) and for total protein concentration:

Proteomics The science of proteomics Applications of proteomics Proteomic methods a. protein purification b. protein sequencing c. mass spectrometry.

An Overview of LabVIEW by: The Software User-Interface Group!

Proteins separation and analysis using Fast Protein Liquid Chromatography Ayelet David, Ph.D Dept. of Clinical Pharmacology.

 Biomolecules are purified using purification techniques that separate according to differences in specific properties.

CHROMATOGRAPHY. A process to separate molecules to purify them.

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC). HIGH PERFORMANCE LIQUID CHROMATOGRAPHY High Performance Liquid Chromatography (HPLC) is one of the most.

Living with the lab Assume that your fishtank system has a setpoint of 0.09% NaCl. Your instructor comes by your table and upsets your system by adding.

Workflow of the Manual Purification of N/NC5-enriched proteins

Why HPLC? Almost universal applicability Remarkable precision Highly commercially available (competition)

High Performance Liquid Chromatography Allyson Lundberg University of Utah November 29, 2006.

Protein Identification using 2D LC/MS/MS based on pH Gradient and Reverse Phase Separation Column Technology Inc.,

. : Introduction: We are testing end-user responses to a potential new chromatography platform for a leading bioprocessing supplier. This new product line.

In the name of God. Common Technical Document On Biotech.

$200 $400 $600 $800 $1000 $200 $400 $600 $800 $1000 $200 $400 $600 $800 $1000 $200 $400 $600 $800 $1000 $200 $400 $600 $800 $1000 $200.

Bioreactor Harvest miniBIOMAN 2017

Purification Of Proteins.

LIQUID CHROMATOGRAPHY

Purification of Green Fluorescent Protein

Chapter 11 -Downstream Processing

Downstream Processing

Automation of Sample Preparation for Trace Analysis Haibin Wan

HPLC Equipment : Detectors

AssayMAP Bravo for Automated Sample Preparation

Chapter 6 Sublimation.

DNA Extraction and Purification

PHCC-National Association

© HiTec Zang GmbH - HRE - 25/07/ GMix41.pps

זכויות סוציאליות.

Pure Carrier Gas Grades (subjective)

INTEGRATED SERVICES IN CHEMICAL DEVELOPMENT

ورود اطلاعات بصورت غيربرخط

Purification of chromogranin B from over-expressing insect sf9 cells.

A Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Protein Precrystallization Screening Motoyuki Hattori,

Purification and characterization of budding yeast cohesin and its loader. Purification and characterization of budding yeast cohesin and its loader. (A)

Volume 76, Issue 4, Pages (April 1999)

Introduction to the NGC Chromatographic System

Downstream Processing

Global Discovery Chemistry, 11 August 2017

Rogert Bauer, Rita Carrotta, Christian Rischel, Lars Øgendal

Gel-filtration chromatography of the active fractions from the Mono-Q column marked by the horizontal bar in Fig. 1. Gel-filtration chromatography of the.

Presentation transcript:

Real-time Concentration Flow VPE Real-time Concentration Analytics During Purification Process Real-time concentration measurements Lab or Pilot version Online total mass Yield and mass balance Characterize membranes Monitor Diafiltration Analog outputs for process automation Cleanable via standard cleaning process Sanitary connections or barb fittings Chromatography UF/DF Real Time Protein Concentration reads easily >125g/L Real Time Mass Integration + 1.5% to Offline UV-Spec Classic UV-Meter maxed out MabSelect Sure Column – Elution Peak FlowVPE attached to Akta CrossFlow 757 Route 202/206 Bridgewater, NJ 08807 (908)707-1009 www.solovpe.com