C. 24-well whole blood (HIV+)

Slides:



Advertisements
Similar presentations
Key Variables in ICS Assays
Advertisements

GTP Scenario # 2 September 17,2005. Scenario # 2 Dr. Good received IND approval for CD8 depletion of allogeneic PBMC for a Phase I/II clinical trial.
Detection of Immune Responses in Mucosal T-cells in Kenya Jackton Indangasi 2, Hannah Cheeseman 1, Onyango J. I. Obila 2, Simon Ogola 2, Robert Langat.
2 pt 3 pt 4 pt 5pt 1 pt 2 pt 3 pt 4 pt 5 pt 1 pt 2pt 3 pt 4pt 5 pt 1pt 2pt 3 pt 4 pt 5 pt 1 pt 2 pt 3 pt 4pt 5 pt 1pt X mixed # equations dividing fractions.
V43 DNA-Ad26-ALVAC Study ELISpot SFC/Million PBMC.
Conversion of Fractions - Mixed Number to Improper Fraction
HIV, a silent destroyer Progression of HIV to AIDS without treatment Human body defense system Body reaction to HIV infection.
Lgr5 RunX1 GATA2 CD34CKITGAPDHLGR4Lmo2 LGR5RunX1 Gata2 CD34 C-kit LGR4 GAPDH Lmo LGR5 GFP Cells E12.5 Liver Gata 2CD 34 C-kit LGR 4 GAPDH Lmo
% % % TcFc1: Expression of CD3, 4 and 8 T cells were gated according to size and granularity to exclude non-vital cells in.
3.What is the principle behind the Quantiferon-Tb assay for TB?
3.4 Slope and Rate of Change Math, Statistics & Physics 1.
CASE 4 54 yo man HIV negative In serodiscordant relationship with untreated HIV positive man…ongoing frequent unprotected sex.
Slope Formula. P1: ( -4, -5) P1: ( x 1, y 1 ) X 1 = -4, y 1 = -5 P2:( 4, 2) P2: (x 2, y 2 ) X 2 = 4, y 2 = 2 ( -4, -5) & ( 4,2)
Slope & Midpoint on the Coordinate Plane. SLOPE FORMULA Given two points (x 1,y 1 ) and (x 2,y 2 ) SLOPE The rate of change to get from one point to another.
Figure S1: kinetics of incubation time and temperature MFI Incubation time (min) B Room temperature +4°C D Room temperature +4°C Incubation time (min)
Reduced Viremia Following Immunotherapy of SIV with Peptide Pulsed Blood Robert De Rose, University of Melbourne, Australia O verlapping P eptide-pulsed.
Human Peripheral Blood Primary Cells Peripheral blood cells are the cellular components of blood, consisting of erythrocytes, leucocytes and platelets,
Side Scatter Forward Scatter Lineage CD1c CD141 CD303 lineage negative
Supplementary Figure 3 Antigen-specific responses are enhanced by stimulation in an in vitro stimulation (IVS) assay, as well as with stimulation using.
Fig. 2. Comparison of the Th1/Th2 ratio and the percentages of Th1 and Th2 cells between normal and allergic individuals. (A) The Th1/Th2 ratio observed.
A. B. EXP #1: 3 donors EXP #2: 3 donors EXP #3: 6 donors EXP #4:
Major Histocompatibility complex OR
GVHD-associated immunodeficiency: soil or seed?
بهداشت فردي.
دانشگاه علوم پزشکی بوشهر برنامه ملی مبارزه با بیماریهای واگیر
HIV targets the immune system.
אידס יום האידס הבנלאומי- אחד בדצמבר לא כדאי למות.... בגלל בורות
Immune System Project : CD28
Mechanisms of HIV-associated lymphocyte apoptosis
Figure 2 Peptide vaccination using TAA-derived long peptides
Sample name Cell line Primary Ab Secondary Ab Comments
مدیریت امور آزمایشگاهها دانشگاه علوم پزشکی کاشان
Interleukin (IL)-1β-producing cells in peripheral blood mononuclear cells (PBMCs). Interleukin (IL)-1β-producing cells in peripheral blood mononuclear.
مقدمه ای بر مهارت های زندگی
آموزش همسان دکتر كامبيز محضري.
Add and Subtract Mixed Numbers
Онлайн ПР за киното Онлайн кино за ПР или.
Depo-Provera altered the expression of cell surface markers associated with HIV susceptibility. Depo-Provera altered the expression of cell surface markers.
Dividing Fractions and Mixed Numbers Guided Notes
ئەيدىز كېسىلى (AIDS) مەمەتئىمىن دوكتۇر.
Rickettsia prowazekii infection of endothelial cells increases leukocyte adhesion through αvβ3 integrin engagement  Y. Bechah, C. Capo, G. Grau, D. Raoult,
Figure 2 Logistical requirements for autologous
Peptide ID# Pulsed to T2-A24 Cells
Қош келдіңіздер.
Supplemental Figure untreated Rituximab Alemtuzumab
STEEL PLATES.
Immunological phenotypes of CD4+ T cells preferentially infected by HIV. PBMCs exposed to HIV-1BaL (MOI 0.01) were cultured for 10 d. Immunological phenotypes.
Tetramer staining performed to determine frequencies of epitope-specific T cells after clearance of HPV16 infection but prior to isolation of T-cell clones.
A comparison of gene transfer and antigen-loaded dendritic cells for the generation of CD4+ and CD8+ cytomegalovirus-specific T cells in HLA-A2+ and HLA-A2−
IBD development correlates with peripheral T cell activation in DNR mice. IBD development correlates with peripheral T cell activation in DNR mice. (A)
KNOCKOUT.
Fig. 1 Effect of preinfection β7Hi CD45RA−CD4+ T cell frequency on HIV acquisition risk in CAPRISA 004 study. Effect of preinfection β7Hi CD45RA−CD4+ T.
Human PBMC-derived MERS-CoV–specific T cells are multifunctional.
Virus-specific T cell responses are detected in all MERS survivors.
Introduction. Activation of HIV-Specific CD8+ T Cells from HIV+ Donors by Vesatolimod.
PD-1 expression on activated regulatory T-cells (aTreg) and CD4+ T-cells. a) Frequencies of aTreg and PD-1+ aTreg in peripheral blood mononuclear cells.
No difference in T-cell response between SLE patients and healthy controls upon superantigen stimulation. No difference in T-cell response between SLE.
Anti-hOX40L treatment prevents aGVHD development in NOG mice transplanted with human PBMCs. NOG mice received human PBMCs (1 × 107) via tail vein. animals.
Adoptive transfer of purified activated G9Cα−/−
Results of blind panel testing.
Representative example of gating on T-cell subpopulations.
Immune cell populations associated with type I IFNGS test status and disease activity. Immune cell populations associated with type I IFNGS test status.
Cytotoxicity of p5399–107-induced CTL cells.
Peptide dose-response curves were done to estimate the avidity of these HTL for peptide TARP1-14 (A) and TARP14-27 (B-E) using autologous PBMCs and dendritic.
HLA-DR53–restricted HTL responses to peptide TARP1-14.
Reproducibility of CD4+ and CD8+ T-cell enumeration with cryopreserved PBMC after variable periods of storage in liquid nitrogen. Reproducibility of CD4+
Analysis of absolute CD4+ cytokine+ cell numbers pre- and post-ART.
Memory phenotypes PBMC-derived MERS-CoV-specific T cells from CWs
Strategies for adoptive immunotherapy of HCMV
Surface CD4 and CD14 and intracellular TNF-α expression in PBMCs
Presentation transcript:

C. 24-well whole blood (HIV+) A. 96-well whole blood B. 96-well PBMC C. 24-well whole blood (HIV+) CD4 0.00 0.25 0.50 0.75 1.00 1.25 CD8 0.0 0.5 1.0 1.5 2.0 2.5 1 2 3 4 CD4 1 2 3 4 CD8 1 2 3 CD8 Peptide mix % IFNg+ cells r 2 = 0.95 r 2 =0.87 r 2 = 0.96 r 2 = 0.73 r 2 = . 7 3 p<0.0001 p<0.0001 p < 0.0001 p = 0.0002 p < . 2 slope 0.94 slope 0.88 slope = 1.2 slope = 1.1 s l o p e = 7 4 plate 0.0 2.5 5.0 7.5 10.0 2 4 6 8 10 12 14 10 20 10 20 30 5 1 SEB % IFNg+ cells 2 r 2 2 =0.95 r 2 = 0.74 r = 0.79 r 2 = . 6 7 r = 0.94 p<0.0001 p = 0.0002 p < 0.0001 p < . 4 p<0.0001 slope 0.93 slope = 0.78 slope = 0.84 s l o p e = . 7 slope 0.91 % IFNg+ cells % IFNg+ cells % IFNg+ cells % IFNg+ cells % IFNg+ cells tube