Selective inhibition of BCL10-induced NF-κB activation by Tat–Peptide VIII HEK-293 cells were transiently co-transfected with an expression vector encoding (A) BCL10 or (B) p65, together with NF-κB–luciferase and β-galactosidase reporter vectors. Selective inhibition of BCL10-induced NF-κB activation by Tat–Peptide VIII HEK-293 cells were transiently co-transfected with an expression vector encoding (A) BCL10 or (B) p65, together with NF-κB–luciferase and β-galactosidase reporter vectors. At 16 h after transfection, cells were treated for 5 h with Tat–Peptide VIII and Tat–control (Peptide IX), at the indicated concentrations. Results shown represent relative luciferase activity normalized to β-galactosidase activity and are representative of six independent experiments performed in triplicate. Daniela Marasco et al. Biochem. J. 2009;422:553-561 ©2009 by Portland Press Ltd