Affinity Chromatography By: Ayesha Naeem

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Presentation transcript:

Affinity Chromatography By: Ayesha Naeem

Chromatography Chromatography is the term of collection of lab techniques for separation of mixtures Derived word Chroma : color Graphein : write

Essential parts of chromatography STATIONARY PHASE: Supports the sample that is to be tested. MOBILE PHASE: That moves in even direction. SAMPLE : That is to be tested.

TYPES OF CHROMATOGRAPHY AFFINITY ION EXCHANGE PARTITION ADSORPTION SIZE EXCLUSION

Here We’ll discuss; AFFINITY CHROMATOGRAPHY Diverse method for the purification of biomolecules and specific molecules from complex compounds Most specifically ; Proteins

Important 2 terms relevant to Affinity Chromatography Matrix Spacer arm Ligand

Examples of affinity chromatography Antigen-antibodies Substrate-enzyme DNA-histon Hormone-receptors

Working Principle Step 1 Step 2 Step 3 Attachment of ligand to column matrix. Step 1 Load mixture into column. Step 2 Binding of specific molecules to the ligands. Step 3

Merci bien

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