PCR and Gel Electrophoresis

Slides:

Advertisements

Similar presentations

PCR way of copying specific DNA fragments from small sample DNA material "molecular photocopying" It’s fast, inexpensive and simple Polymerase Chain Reaction.

Advertisements

Polymerase Chain Reaction (PCR). PCR produces billions of copies of a specific piece of DNA from trace amounts of starting material. (i.e. blood, skin.

DNA Fingerprinting and Forensic Analysis Chapter 8.

Biotech Continued… How do forensic scientists determine who’s blood has been left at a crime scene? How do forensic scientists determine who’s blood.

The polymerase chain reaction (PCR) rapidly

Advanced Molecular Biological Techniques. Polymerase Chain Reaction animation.

Polymerase Chain Reaction (PCR)

Ch. 13.4: DNA Technology Applications

Recombinant DNA Technology………..

Polymerase Chain Reaction Mrs. Stewart Medical Interventions.

What do these terms mean to you? You have 5 min to discuss possible meanings and examples with your group! DNA sequencing DNA profiling/fingerprinting.

Polymerase Chain Reaction (PCR) Developed in 1983 by Kary Mullis Major breakthrough in Molecular Biology Allows for the amplification of specific DNA fragments.

Success criteria - PCR By the end of this lesson we will be able to: 1. The polymerase chain reaction (PCR) is a technique for the amplification ( making.

It is a technique used to produce large quantities of replicated DNA.

6.3 Advanced Molecular Biological Techniques 1. Polymerase chain reaction (PCR) 2. Restriction fragment length polymorphism (RFLP) 3. DNA sequencing.

Advantages of STR Analysis

Biology Chapter 9 & Honors Biology Chapter 13 Frontiers Of Biotechnology.

PCR – Polymerase Chain Reaction A method of amplifying small amounts of DNA using the principles of DNA replication.

PCR Polymerase Chain Reaction. What is it and how is it useful?  A quick, easy method for amplifying (creating billions of copies) of unimaginably tiny.

Crime Scene Investigator PCR Basics™

Higher Human Biology Unit 1 Human Cells KEY AREA 5: Human Genomics.

Polymerase Chain Reaction (PCR). What’s the point of PCR? PCR, or the polymerase chain reaction, makes copies of a specific piece of DNA PCR allows you.

Copying DNA: The Polymerase Chain Reaction. The Polymerase Chain Reaction (PCR) POINT > Explain why copying DNA is useful POINT > Define PCR POINT > Describe.

DNA Deoxyribonucleic Acid. DNA Review Genetic material (DNA) is found in the nucleus of cells, and is contained on chromosomes. An organism inherits chromosomes.

PCR and Gel Electrophoresis The Polymerase Chain Reaction –Denaturation, Annealing, Elongation Gel Electrophoresis Application in forensic science.

1/03VisTE Project - Copyright 2002 Biotechnology The Polymerase Chain Reaction (PCR)

PCR Polymerase chain reaction. PCR is a method of amplifying (=copy) a target sequence of DNA.

Presented by: Khadija Balubaid.  PCR (Polymerase Chain Reaction) is a molecular biological technique  used to amplify specific fragment of DNA in vitro.

Protein Synthesis - Transcription

Polymerase Chain Reaction

Success criteria - PCR By the end of this lesson we will be know:

Biogenetic Engineering

DNA Organization and Aging

Today’s Title: CW: DNA manipulation – separating and probing

Molecular Cloning: Polymerase Chain Reaction

Polymerase Chain Reaction

Polymerase Chain Reaction

PCR uses polymerases to copy DNA segments.

PCR and RLFP’s.

Polymerase Chain Reaction & DNA Profiling

BIOTECHNOLOGY BIOTECHNOLOGY: Use of living systems and organisms to develop or make useful products GENETIC ENGINEERING: Process of manipulating genes.

Polymerase Chain Reaction

3. PCR Page 376 – 377.

Biogenetic Engineering

Polymerase Chain Reaction (PCR) technique

Biogenetic Engineering

The student is expected to: (6H) describe how techniques such as DNA fingerprinting, genetic modifications, and chromosomal analysis are used to study.

BIOTECHNOLOGY PART 2.

Polymerase Chain Reaction

PCR -PCR replicates (or amplifies) the DNA many times so that a large enough sample can be analyzed.

Recombinant DNA Unit 12 Lesson 2.

PCR uses polymerases to copy DNA segments.

PCR uses polymerases to copy DNA segments.

Polymerase Chain Reaction (PCR) & DNA SEQUENCING

Polymerase Chain Reaction (PCR).

Polymerase Chain Reaction (PCR)

Introduction to Polymerase Chain Reaction (PCR)

DNA Replication Notes.

Polymerase Chain Reaction (PCR)

PCR Polymerase chain reaction (PCR)

Biotechnology Part 2.

PCR uses polymerases to copy DNA segments.

Polymerase Chain Reaction

3.5 Genetic modification and biotechnology

PCR uses polymerases to copy DNA segments.

PCR uses polymerases to copy DNA segments.

POLYMERASE CHAIN REACTION

Polymerase Chain Reaction (PCR) & DNA SEQUENCING

PCR uses polymerases to copy DNA segments.

Presentation transcript:

PCR and Gel Electrophoresis The Polymerase Chain Reaction Denaturation, Annealing, Elongation Gel Electrophoresis Application in forensic science

The Polymerase Chain Reaction A process that is used to make a huge number of copies of a DNA sequence in a laboratory, quickly and without the need for a host organism PCR is DNA replication of a specific region of the genome, outside of the nucleus of a cell ____________

TeachWithFergy Preview File Please enjoy this preview of your Power Point. Some slides appear blank because they have been removed. Other slides may have ........... on them, this represents writing that has been removed. Please note that the Entire Unit Package can also be purchased at a steep discount from my Store.

Step 1 - Denaturation Cycle 1 ____________ This occurs when the DNA strand is heated to 94 to 96 °C for 20 to 40s, causing the hydrogen bonds holding the two strands together to break.

This slide has been removed

Step 3 - Elongation Elongation: Heat to 72°C for DNA polymerase (Taq polymerase) to extend the primers using free-floating nucleotide precursors to make complementary copies of each template strand. Taq polymerase is a special DNA polymerase found in the bacteria thermus aquaticus. __________ Note, ……………..

This slide has been removed

Cycle 3 Repeat the same steps again ____________ Once this repeats 20 times, most of the segments will be pure target DNA

This slide has been removed

Summary ____________ There is enough DNA in a hair follicle or a tiny blood spatter to perform PCR. Once the DNA of interest has been amplified using PCR, it is analyzed using different methods

Gel Electrophoresis Once the targeted DNA sequence has been amplified by PCR, it needs to be separated and purified from the rest of the contents in the test tube. ____________

This slide has been removed

Step 1

Step 2 A molecular marker is a DNA fragment of known size that is used to determine the lengths of unknown fragments by comparison (a standard) ____________

This slide has been removed

Step 4 To be able to see ……………

Why? Using gel electrophoresis, researchers can compare the size of the DNA fragments from an unknown sample to the size of the DNA fragments from a known sample ____________ These conclusions may include: …………….. the identity of the perpetrator of a crime ……………….

How? ___________ No two people will have the same number of bases in those areas Example Target Sequence # of bases for Susan # of bases for Jim A 100 115 B 56 77

This slide has been removed

Who is the daddy?

However, its looks more like this. Who is guilty???

Video has been removed. In the full version, you simply click on the video link and it opens for you. NOVA Simulation