Do Now 3/13 Objectives: Describe the importance of PCR.

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Presentation transcript:

Do Now 3/13 Objectives: Describe the importance of PCR. Explain how PCR works Tasks: Given the coding DNA sequence: 5’ AAT-CCG-ATA 3’ Determine the associated template strand and mRNA

Exponential Growth

Key Ideas from Last Night’s Homework Transformation: prokaryotes acquire DNA from the environment. Transduction: phages transfer DNA between prokaryotes Conjugation: prokaryotic cells exchange genetic material. Plasmid: tiny bonus chromosome in some prokaryotic cells

Transformation by environmental DNA Conjugation transfers plasmid DNA Transduction of phage DNA into chromosome or plasmid

PCR: Polymerase Chain Reaction WHAT IT IS: A way to make a large number (billions) of copies of a DNA sequence in a few hours. WHY IT MATTERS: provides enough DNA to do research with. WHEN: 1986. Totally revolutionized biology.

How it Works

Virtual PCR http://learn.genetics.utah.edu/content/labs/pcr/ Youtubes…

One last note… Thermus aquaticus The ability to perform PCR is predicated on a thermostable DNA polymerase that functions at a high temperature. Taq is a prokaryote from hot springs. Biotech = prokaryotes!!! 

Your Assignments Reading on syllabus ADD: PCR questions on back of diagram.