Photosynthesis

Photosynthesis light reactions (thylakoid) 6CO2 + 12 H2O gives C6H12O6 + 6O2 + 6H2O Calvin cycle or dark reactions (stroma) 􀁡photoenergy is required (endergonic

Using the Spectroscope 􀁡view white light w/ spectroscope,record colors seen 􀁡view red, blue, and green filters w/ Spectroscope,record colors seen 􀁡view spinach extract w/ spectroscope,record colors seen

Using the Spectrophotometer tube 1 spinach extract tube 2 (blank) solvent tube 3 carrot extract 􀁡 record %T from 400-700 nm in 25nm increments, graph 􀁡 100% T must be reset whenever λ is changed % %

Chromatography solvent line carotenes = orange carotenoids xanthophylls = yellow chlorophyll a = blue-green chlorophylls chlorophyll b = yellow-green baseline

Chromatography, Rf values 􀁡Rf = pigment distance/solvent distance 􀁡affinity for the solvent = high Rf value 􀁡affinity for the paper = low Rf value 􀁡Staple finished chromatograms to report

Starch localization in leaves 􀁡Boiling the leaf in alcohol will remove the pigments, it will turn white 􀁡flood leaf w/ iodine iodine + starch = blue-black Draw leaf in color before boiling and after staining.

Chloroplast Model Label chloroplast in your lab manual 􀁡outer membrane 􀁡inner membrane 􀁡intermembrane space 􀁡stroma (Calvin cycle or dark reactions) 􀁡thylakoid (light reactions), granum, grana