In Vitro Regulation of CCL3 and CXCL12 by Bacterial By-products Is Dependent on Site of Origin of Human Oral Fibroblasts Carla Renata Sipert, DDS, PhD,

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In Vitro Regulation of CCL3 and CXCL12 by Bacterial By-products Is Dependent on Site of Origin of Human Oral Fibroblasts Carla Renata Sipert, DDS, PhD, Ana Carolina Morandini, DDS, PhD, Thiago José Dionísio, MSc, Maria Aparecida Andrade Moreira Machado, DDS, PhD, Sandra Helena Penha Oliveira, PhD, Ana Paula Campanelli, PhD, Winston Patrick Kuo, DDS, DMSc, Carlos Ferreira Santos, DDS, PhD Journal of Endodontics Volume 40, Issue 1, Pages 95-100 (January 2014) DOI: 10.1016/j.joen.2013.09.031 Copyright © 2014 American Association of Endodontists Terms and Conditions

Figure 1 Characterization of cultured fibroblasts by FSP-1. Cultured GF (A), PLF (B), PDPF (C), and DDPF (D) were immunostained for FSP-1. Images captured by a confocal microscope (representative bars, 20 μm). Journal of Endodontics 2014 40, 95-100DOI: (10.1016/j.joen.2013.09.031) Copyright © 2014 American Association of Endodontists Terms and Conditions

Figure 2 Production of CCL3 (MIP-1α) by cultured fibroblasts. Cultured GF, PLF, PDPF, and DDPF were stimulated with EcLPS (A–C) and EfLTA (D–F) at the indicated concentrations. Cell supernatants were collected after 1 hour (A and D), 6 hours (B and E), and 24 hours (C and F). Production of CCL3 was detected by ELISA. *P < .05, **P < .01, and ***P < .001 in comparison with culture medium alone (0). Significant differences with #GF, with %PLF, and with &PDPF (n = 6). Journal of Endodontics 2014 40, 95-100DOI: (10.1016/j.joen.2013.09.031) Copyright © 2014 American Association of Endodontists Terms and Conditions

Figure 3 Production of CXCL12 (SDF-1) and expression of miR-141 and miR-200a by cultured fibroblasts. Cultured GF, PLF, PDPF, and DDPF were stimulated with EcLPS (A–C) and EfLTA (D–F) at the indicated concentrations. Cell supernatants were collected after 1 hour (A and D), 6 hours (B and E), and 24 hours (C and F). Production of CXCL12 was detected by ELISA. *P < .05, **P < .01, and ***P < .001 in comparison with culture medium alone (0). Significant differences with #GF, with %PLF, and with &PDPF (n = 6). Expression of miR-141 (G) and miR-200a (H) was detected by means of qPCR for EcLPS-stimulated cells for 24 hours (n = 6). Journal of Endodontics 2014 40, 95-100DOI: (10.1016/j.joen.2013.09.031) Copyright © 2014 American Association of Endodontists Terms and Conditions