Behzad Kasravi, M. D. , Ph. D. , Diana H. Lee, B. S. , Jean W. Lee, B

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Presentation transcript:

Chylomicron-Bound LPS Selectively Inhibits the Hepatocellular Response to Proinflammatory Cytokines Behzad Kasravi, M.D., Ph.D., Diana H. Lee, B.S., Jean W. Lee, B.S., Stephen Dada, M.D., Hobart W. Harris, M.D., M.P.H. Journal of Surgical Research Volume 146, Issue 1, Pages 96-103 (May 2008) DOI: 10.1016/j.jss.2007.06.013 Copyright © 2008 Elsevier Inc. Terms and Conditions

FIG. 1 Effect of different pretreatments on NO production in primary rat hepatocytes and McArdle cells. Open bars represent primary rat hepatocytes and shaded bars represent McArdle cells; control, non-pretreated and stimulated; LPS, endotoxin alone; CM, chylomicron alone; CM-LPS, chylomicron-endotoxin complexes. Data represent the mean ± SEM of six samples from two separate experiments (*P ≤ 0.05 compared with their respective controls, t-test). Journal of Surgical Research 2008 146, 96-103DOI: (10.1016/j.jss.2007.06.013) Copyright © 2008 Elsevier Inc. Terms and Conditions

FIG. 2 Effect of CM-LPS pretreatment on the hepatocellular response to oxidative stress. Open bars represent cells with no pretreatment, shaded bars represent cells pretreated with CM-LPS. Mitochondrial activity is shown as a percent of unstimulated control. There was no significant difference between the CM-LPS pretreated and non-pretreated groups. Data represent the mean ± SEM of three samples per condition from two separate experiments (*P ≤ 0.05 compared with 0 time point, t-test). Journal of Surgical Research 2008 146, 96-103DOI: (10.1016/j.jss.2007.06.013) Copyright © 2008 Elsevier Inc. Terms and Conditions

FIG. 3 Effect of CM-LPS pretreatment on the hepatocellular response to hypoxia induced with 200 μM CoCl2. This immunoblot of the nuclear fraction is a representative sample from two experiments. Journal of Surgical Research 2008 146, 96-103DOI: (10.1016/j.jss.2007.06.013) Copyright © 2008 Elsevier Inc. Terms and Conditions

FIG. 4 Effect of CM-LPS pretreatment on the hepatocellular response to heat stress. This immunoblot of cytoplasmic and nuclear HSP 25, 32, and 72 in pretreated and non-pretreated hepatocytes after heat shock (43°C × 45 min) is a representative sample of three separate experiments. Journal of Surgical Research 2008 146, 96-103DOI: (10.1016/j.jss.2007.06.013) Copyright © 2008 Elsevier Inc. Terms and Conditions

FIG. 5 Effect of CM-LPS on induction of thermotolerant phenotype in primary hepatocytes. Data represent the mean ± SEM of mitochondrial activity measured by AlamarBlue assay of nine samples from three separate experiments. Journal of Surgical Research 2008 146, 96-103DOI: (10.1016/j.jss.2007.06.013) Copyright © 2008 Elsevier Inc. Terms and Conditions

FIG. 6 Effect of CM-LPS pretreatment on the enzymatic activity of (A) caspase 3 and (B) caspase 8 stimulation with TNF-α (2000 U/mL) and actinomycin D (400 nm) in pretreated and non-pretreated cells. Caspase 3 (20 kDa and 17 kDa bands) and caspase 8 (43 kDa and 41 kDa bands). Journal of Surgical Research 2008 146, 96-103DOI: (10.1016/j.jss.2007.06.013) Copyright © 2008 Elsevier Inc. Terms and Conditions

FIG. 7 Effect of CM-LPS pretreatment on the enzymatic activity of the apoptotic enzymes (A) caspase 3 and (B) caspase 8. Open bars represent cells with no pretreatment and shaded bars represent cells pretreated with CM-LPS. Data represent the mean ± SEM of six samples from two separate experiments (*P ≤ 0.05 compared with their respective 0 h controls, t-test). Journal of Surgical Research 2008 146, 96-103DOI: (10.1016/j.jss.2007.06.013) Copyright © 2008 Elsevier Inc. Terms and Conditions

FIG. 8 Effect of different cytokines on primary rat hepatocytes pretreated with CM-LPS. Data represent mean ± SEM of six samples from two different experiments (*P < 0.05 compared with corresponding non-pretreated groups, t-test). Journal of Surgical Research 2008 146, 96-103DOI: (10.1016/j.jss.2007.06.013) Copyright © 2008 Elsevier Inc. Terms and Conditions

FIG. 9 Effect of different cytokines on transformed McArdle cells. Data represent mean ± SEM of six samples from two different experiments (*P < 0.05 compared with corresponding non-pretreated group, t-test). Journal of Surgical Research 2008 146, 96-103DOI: (10.1016/j.jss.2007.06.013) Copyright © 2008 Elsevier Inc. Terms and Conditions