Forensic DNA typing strategy of degraded DNA on discarded cigarette ends using the AmpFℓSTR® Identifiler®, Identifiler® Plus and MiniFiler™ PCR Amplification.

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Presentation transcript:

Forensic DNA typing strategy of degraded DNA on discarded cigarette ends using the AmpFℓSTR® Identifiler®, Identifiler® Plus and MiniFiler™ PCR Amplification Kits Stephen C.Y. Ip, Sze-wah Lin, Christina Li, Kam-ming Lai Science and Justice Volume 54, Issue 4, Pages 311-315 (July 2014) DOI: 10.1016/j.scijus.2014.04.007 Copyright © 2014 Forensic Science Society Terms and Conditions

Fig. 1 An overview of Short Tandem Repeat (STR) typing results on the dry cigarette ends upon analysis with (A) Identifiler®, (B) Identifiler® Plus, and (C) MiniFiler™. A horizontal slice represents results from a single sample. White box indicates the detection of a homozygous allele with peak height above the stochastic threshold or two heterozygote alleles. Light grey box indicates the detection of a single allele below the stochastic threshold. Dark grey box indicates no detection of any allele. Pos and Neg indicate positive and negative controls, respectively. An asterisk (*) indicates the samples with incomplete Identifiler® profiles selected for further testing with Identifiler® Plus and MiniFiler™ as shown in (B) and (C) respectively. Science and Justice 2014 54, 311-315DOI: (10.1016/j.scijus.2014.04.007) Copyright © 2014 Forensic Science Society Terms and Conditions

Fig. 2 An overview of the short tandem repeat (STR) typing results on the wet cigarette ends upon analysis with (A) Identifiler®, (B) Identifiler® Plus, and (C) MiniFiler™. Pos and Neg indicate positive and negative controls, respectively. A hash (#) indicates the identity of the sample. An asterisk (*) indicates the samples with incomplete Identifiler® profiles selected for further testing with Identifiler® Plus and MiniFiler™ as shown in (B) and (C) respectively. Science and Justice 2014 54, 311-315DOI: (10.1016/j.scijus.2014.04.007) Copyright © 2014 Forensic Science Society Terms and Conditions

Fig. 3 The relationship between Identifiler® peak height imbalance observed at the loci and their peak heights. Science and Justice 2014 54, 311-315DOI: (10.1016/j.scijus.2014.04.007) Copyright © 2014 Forensic Science Society Terms and Conditions

Fig. 4 Number of contributor(s) detected for the cigarette end samples. Science and Justice 2014 54, 311-315DOI: (10.1016/j.scijus.2014.04.007) Copyright © 2014 Forensic Science Society Terms and Conditions

Fig. 5 The relationship between MiniFiler™ peak height imbalance observed at the loci and their peak heights. Science and Justice 2014 54, 311-315DOI: (10.1016/j.scijus.2014.04.007) Copyright © 2014 Forensic Science Society Terms and Conditions