From: Engrafted Chicken Neural Tube–Derived Stem Cells Support the Innate Propensity for Axonal Regeneration within the Rat Optic Nerve Invest. Ophthalmol.

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From: Engrafted Chicken Neural Tube–Derived Stem Cells Support the Innate Propensity for Axonal Regeneration within the Rat Optic Nerve Invest. Ophthalmol. Vis. Sci.. 2008;49(8):3513-3524. doi:10.1167/iovs.07-1473 Figure Legend: Embryonic NTSC markers. (A–C) immunocytochemistry with nestin-antibody (A) revealed that most of the cells seen in phase-contrast (B) were stainable (C). (D) Quantification of different markers revealed that only 2% were β-III-tubulin and 2.4% MAP-2 positive; 49.5% expressed GFAP, and 80% were nestin-positive. (E) The TRAP-assay showed that the ratio between the positive and negative control was >0.2 throughout the time of culture up to 60 days. (F–H) Staining with antibodies to bFGF revealed that NTSCs were positive and indicated that the cells produced bFGF for their own survival. Date of download: 10/10/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.