Innovative Analytical Solutions for the Dairy Industry

Bentley CombiFTS Rapid and accurate determination of milk chemical composition and somatic cells content Milchprüfring Baden-Württemberg, Germany 5xCombiFTS 500, 1 IBC150

Bentley Bartec CombiFTS 600 Up to 600 samples/hr

Bentley Bartec CombiFTS 500

Bentley ILAS CombiFTS 600 Up to 600 samples/hr

Bentley ILAS CombiFTS 600 The Optimum Solution Fully Automated Warmed up in a water bath, shaken and identified (RFID, Barcode) Uncapped Samples are replaced in the tray after analysis Pilot samples automatically inserted in the process

Bentley CombiFTS Somacount FCM (Flow Cytometry) Bentley FTS (FTIR)

Bentley FTS Milk Component Absorption Wavebands Lactose (OH) Protein (NH) Fat A (C=0) Fatty Acids Fat B (CH) Lactose (OH) Frequency of infrared radiation absorbed depending upon type of molecular bonds /vibration mode

Recordings from the Instrument Infrared Spectrum Somatic cells peak distributions Environmental variables could affect the end results Sample, water bath, ambient, homogenizer, deck temperatures Optical deck Moisture level and pressure Centerburst energy level (automatic alignment) Homogenizar pressure Vibrations

FTS Infrared Applications Traditional Components New Components New Developments Blood BHB Lactoferrin Iodine Value Fatty Acids calibrations Fat Protein Lactose Solids SNF MUN FDP Citric Acid Casein pH FFA Fatty Acids Profile Ketone Bodies

Blood BHB Monitoring of the cows metabolic activity and detection of ketosis by infrared spectroscopy Why predict Blood BHB instead of Milk BHB? Milk BHB is unstable and levels very low Milk BHB content will decrease rapidly if the milk is not stored at 4°C right after milking The development of a calibration based on the Blood BHB allows not only to take into account the variation of the ketone bodies but also all the variations in the milk composition that are directly or indirectly linked to the metabolic disorder, and particularly the milk fatty acids profile

Blood BHB Calibration on 696 cow samples

Blood BHB Correlation spectrum between Blood BHB content & milk spectra The spectral zone with the highest correlation are related to the milk fatty acids profile

Lactoferrin Used for early detection of mastitis & monitoring of the sanitary status of the mammary gland Iron binding antimicrobial glycoprotein Plays a role in pre-immune host defense Strong association between LF & occurrence of mastitis Used to check sanitary status of the mammary glands in the herd

Lactoferrin LFir (µg/mL) 77,13 0,21 0,22 0,65 0,64 0,68 LFir (µg/mL)   LFir (µg/mL) LFref Log LFir Log LFref (µg/mL) IBC/µL Log IBC/µL SCC/µL Log SCC/µL Healthy 144,04 120,85 1,86 1,99 314 2,34 95 1,88 Accute 210,32 234,46 2,09 2,33 5592 3,53 1967 3,19 Chronic 226,98 250,66 2,15 2,38 8273 3,74 2863 3,31 Min 41 15 1,13 1,17 63 1,80 11 1,04 Max 413 412 2,45 2,62 22082 4,34 9999 4,00 Mean 194 202 2,04 2,23 4726 3,20 1642 2,79 Sd 66,02 99,03 0,24 0,28 6213,44 0,74 2242,39 0,72 Correlation between Log LFir and Log SCC with Log LFref significantly better than normal scale Correlation between Log (Lfir) and Log(LFref) equivalent to correlation between Log SCC and Log LFref LF seems to be a good indicator of Cow Mastitis Status and could especially be used to differentiate healthy from accute or chronical mastitis It has been suggested that bovine milk LF play an important role in host defense against Escherichia Coli mastitis, On the other end, LF thought to have little antibacterial effect on Staphylococcus aureus or Streptococcus agalactia LFir (µg/mL) LFRef (µg/mL) logLFir LogLFRef IBC/µL Log IBC/µL SCC/µL Log SCC/µL Sy,x/LFref 77,13   0,21 0,26 0,22 0,25 r/Log LFRef 0,65 1,00 0,42 0,64 0,43 0,68

Lactoferrin