Journal of Molecular and Cellular Cardiology

Slides:

Advertisements

Similar presentations

Date of download: 5/28/2016 Copyright © The American College of Cardiology. All rights reserved. From: Contractile Function During Angiotensin-II Activation:

Advertisements

Date of download: 6/3/2016 Copyright © The American College of Cardiology. All rights reserved. From: Increased Expression of Mineralocorticoid Receptor.

The chemokine CCL2 activates p38 mitogen-activated protein kinase pathway in cultured rat hippocampal cells Jungsook Cho, Donna L. Gruol Journal of.

Nicotinamide Phosphoribosyltransferase: A Potent Therapeutic Target in Non-small Cell Lung Cancer with Epidermal Growth Factor Receptor-Gene Mutation

Sirt1 modulates premature senescence-like phenotype in human endothelial cells Hidetaka Ota, Masahiro Akishita, Masato Eto, Katsuya Iijima, Masao Kaneki,

EGCG prevents PCB-126-induced endothelial cell inflammation via epigenetic modifications of NF-κB target genes in human endothelial cells Dandan Liu,

Reduction of heart failure by pharmacological inhibition or gene deletion of protein tyrosine phosphatase 1B Elodie Gomez, Magali Vercauteren, Baptiste.

Sarcolemmal Ca2+-entry through L-type Ca2+ channels controls the profile of Ca2+- activated Cl− current in canine ventricular myocytes Balázs Horváth,

Journal of Molecular and Cellular Cardiology

Journal of Molecular and Cellular Cardiology

by Yingmei Zhang, Ming Yuan, Katherine M

Dapper-1 Induces Myocardial Remodeling Through Activation of Canonical Wnt Signaling in CardiomyocytesNovelty and Significance by Marco Hagenmueller, Johannes.

Mitogen‐Activated Protein Kinase and Intracellular Polyamine Signaling Is Involved in TRPV1 Activation–Induced Cardiac Hypertrophy by Mai Chen, Jiajia.

IP3 Receptor Activity Is Differentially Regulated in Endoplasmic Reticulum Subdomains during Oocyte Maturation Michael J. Boulware, Jonathan S. Marchant

Low-intensity pulsed ultrasound (LIPUS) treatment of cultured chondrocytes stimulates production of CCN family protein 2 (CCN2), a protein involved in.

Volume 99, Issue 3, Pages (October 1999)

Glutamate signaling in chondrocytes and the potential involvement of NMDA receptors in cell proliferation and inflammatory gene expression T. Piepoli,

C.Allyson Walker, BA, Francis G. Spinale, MD, PhD

Getting Pumped about Heart Failure

Mg2+ dependence of Ca2+ release from the sarcoplasmic reticulum induced by sevoflurane or halothane in skeletal muscle from humans susceptible to malignant.

Volume 57, Issue 1, Pages (January 2000)

Monitoring the Structural Behavior of Troponin and Myoplasmic Free Ca2+ Concentration during Twitch of Frog Skeletal Muscle Tatsuhito Matsuo, Hiroyuki.

R. L. Damatto, P. F. Martinez, A. R. R. Lima, M. D. M. Cezar, D. H. S

Volume 12, Issue 12, Pages (September 2015)

Cardiac remodeling and dysfunction in nephrotic syndrome

NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes Dr C. Lianxu, Ph.D.,

Repeated remote ischemic conditioning attenuates left ventricular remodeling via exosome-mediated intercellular communication on chronic heart failure.

L. Raymond, S. Eck, E. Hays, I. Tomek, M. D. , S. Kantor, M. D. , M

Volume 5, Issue 6, Pages (December 2015)

Walking Modulates Speed Sensitivity in Drosophila Motion Vision

Volume 131, Issue 4, Pages (November 2007)

Morphogens: Precise Outputs from a Variable Gradient

Low-intensity pulsed ultrasound (LIPUS) treatment of cultured chondrocytes stimulates production of CCN family protein 2 (CCN2), a protein involved in.

Etienne Roux, Marko Marhl Biophysical Journal

Volume 99, Issue 3, Pages (August 2010)

Jeffrey G. Jacot, Andrew D. McCulloch, Jeffrey H. Omens

A.C. McMahon, R.U. Naqvi, M.J. Hurst, A.E.G. Raine, K.T. MacLeod

Volume 14, Issue 2, Pages (August 2011)

Elisa Bovo, Stefan R. Mazurek, Pieter P. de Tombe, Aleksey V. Zima

Volume 100, Issue 10, Pages (May 2011)

Volume 19, Issue 3, Pages (June 2012)

Estrogen enhancement of SGK1 expression induced by urocortin contributes to its cardioprotection against ischemia/reperfusion insult Binhai Cong, Jiankui.

Christopher A. Ward, Hongqun Liu, Samuel S. Lee Gastroenterology

Volume 132, Issue 4, Pages (April 2007)

Marinus A. Borgdorff, Beatrijs Bartelds, Michael G

Volume 70, Issue 1, Pages (July 2006)

Volume 5, Issue 6, Pages (December 2015)

Elen Rizzi, Michele M. Castro, Carla S. Ceron, Evandro M

Alisson Martins-Oliveira, Michele M. Castro, Diogo M. M

Induction of heart failure by minimally invasive aortic constriction in mice: Reduced peroxisome proliferator-activated receptor γ coactivator levels.

Walking Modulates Speed Sensitivity in Drosophila Motion Vision

Cell therapy prevents structural, functional and molecular remodeling of remote non- infarcted myocardium Leonardo dos Santos, Giovana A. Gonçalves, Ana.

Glutamate signaling in chondrocytes and the potential involvement of NMDA receptors in cell proliferation and inflammatory gene expression T. Piepoli,

Prachi Umbarkar et al. BTS 2019;j.jacbts

Adenosine Prevents Hyperkalemia-Induced Calcium Loading in Cardiac Cells: Relevance for Cardioplegia Aleksander Jovanović, MD, Alexey E Alekseev, PhD,

Stephan D. Brenowitz, Wade G. Regehr Neuron

Heme oxygenase: protective enzyme or portal hypertensive molecule?

In vitro differences between smooth muscle cells derived from varicose veins and normal veins Ying Xiao, PhD, Zhibin Huang, MD, Henghui Yin, PhD, Ying.

Volume 75, Issue 4, Pages (October 1998)

Josée Lamoureux, PhD, Jana Stankova, PhD, Marek Rola-Pleszczynski, MD

Volume 110, Issue 6, Pages (September 2002)

Volume 21, Issue 22, Pages (November 2011)

Transient Receptor Potential Vanilloid-1 Mediates Heat-Shock-Induced Matrix Metalloproteinase-1 Expression in Human Epidermal Keratinocytes Wen H. Li,

Volume 86, Issue 4, Pages (April 2004)

Dexamethasone suppresses gene expression and production of IL-13 by human mast cell line and lung mast cells Toshiaki Fushimi, MD, Hiroshi Okayama, MD,

Fig. 4. Representative Western blots for phospholamban, Na+/Ca2+ exchanger, sarco(endo)plasmic reticulum calcium- ATPase (SERCA2), and dihydropyridine.

Assessment of Sarcoplasmic Reticulum Ca2+ Depletion During Spontaneous Ca2+ Waves in Isolated Permeabilized Rabbit Ventricular Cardiomyocytes N. MacQuaide,

Myocardial function after fetal cardiac bypass in an ovine model

Jeffrey G. Jacot, Andrew D. McCulloch, Jeffrey H. Omens

George D. Dickinson, Ian Parker Biophysical Journal

Presentation transcript:

Journal of Molecular and Cellular Cardiology The benefits of endurance training in cardiomyocyte function in hypertensive rats are reversed within four weeks of detraining Miguel Araujo Carneiro-Júnior, Judson Fonseca Quintão-Júnior, Lucas Rios Drummond, Victor Neiva Lavorato, Filipe Rios Drummond, Daise Nunes Queiroz da Cunha, Marco Aurélio Amadeu, Leonardo Bonato Felix, Edilamar Menezes de Oliveira, Jader Santos Cruz, Thales Nicolau Prímola-Gomes, José Geraldo Mill, Antonio José Natali Journal of Molecular and Cellular Cardiology Volume 57, Pages 119-128 (April 2013) DOI: 10.1016/j.yjmcc.2013.01.013 Copyright © 2013 Elsevier Ltd Terms and Conditions

Fig. 1 Representative line-scan images recorded from field-stimulated cardiomyocytes loaded with the Ca2+ indicator fluo 4-AM (5μM). Ca2+ signal is shown as fluorescence ratio (F/F0), with the fluorescence intensity (F) normalized to intensity at rest before stimulation (F0). NC8, normotensive control for 8weeks. NT8, normotensive trained for 8weeks. HC8, hypertensive control for 8weeks. HT8, hypertensive trained for 8weeks. NC12, normotensive control for 12weeks. NDT, normotensive trained for 8 and detrained for 4weeks. HC12, hypertensive control for 12weeks. HDT, hypertensive trained for 8 and detrained for 4weeks. Journal of Molecular and Cellular Cardiology 2013 57, 119-128DOI: (10.1016/j.yjmcc.2013.01.013) Copyright © 2013 Elsevier Ltd Terms and Conditions

Fig. 2 Cardiomyocyte calcium transients. (A) Amplitude of transients. (B) Time to peak. (C) Time from peak transient to half resting value. NC8, normotensive control for 8weeks. NT8, normotensive trained for 8weeks. HC8, hypertensive control for 8weeks. HT8, hypertensive trained for 8weeks. NC12, normotensive control for 12weeks. NDT, normotensive trained for 8 and detrained for 4weeks. HC12, hypertensive control for 12weeks. HDT, hypertensive trained for 8 and detrained for 4weeks. Data are mean±SEM of 70–80 cells in each group. ⁎, significantly different from NC8. #, significantly different from HC8. ¨ significantly different from NT8. †, significantly different from NC12. ‡, significantly different from NDT. Journal of Molecular and Cellular Cardiology 2013 57, 119-128DOI: (10.1016/j.yjmcc.2013.01.013) Copyright © 2013 Elsevier Ltd Terms and Conditions

Fig. 3 Relationship between total exercise time until fatigue (TTF) and [Ca2+]i transient amplitude, time to peak and time from peak transient to half resting in normotensive (A, C and E) and SHR (B, D and F) animals. Open/filled triangles and circles represent the means of individual SHR and normotensive rats. NC8, normotensive control for 8weeks. NT8, normotensive trained for 8weeks. HC8, hypertensive control for 8weeks. HT8, hypertensive trained for 8weeks. NC12, normotensive control for 12weeks. NDT, normotensive trained for 8 and detrained for 4weeks. HC12, hypertensive control for 12weeks. HDT, hypertensive trained for 8 and detrained for 4weeks. Journal of Molecular and Cellular Cardiology 2013 57, 119-128DOI: (10.1016/j.yjmcc.2013.01.013) Copyright © 2013 Elsevier Ltd Terms and Conditions

Fig. 4 Representative records of cardiomyocytes contractions. Values are percentages of resting cell length (% r.c.l.). NC8, normotensive control for 8weeks. NT8, normotensive trained for 8weeks. HC8, hypertensive control for 8weeks. HT8, hypertensive trained for 8weeks. NC12, normotensive control for 12weeks. NDT, normotensive trained for 8 and detrained for 4weeks. HC12, hypertensive control for 12weeks. HDT, hypertensive trained for 8 and detrained for 4weeks. Journal of Molecular and Cellular Cardiology 2013 57, 119-128DOI: (10.1016/j.yjmcc.2013.01.013) Copyright © 2013 Elsevier Ltd Terms and Conditions

Fig. 5 Cardiomyocyte contractile function. (A) Cell shortening expressed as % of resting cell length. (B) Maximal velocity of shortening. (C) Maximal velocity of relengthening. NC8, normotensive control for 8weeks. NT8, normotensive trained for 8weeks. HC8, hypertensive control for 8weeks. HT8, hypertensive trained for 8weeks. NC12, normotensive control for 12weeks. NDT, normotensive trained for 8 and detrained for 4weeks. HC12, hypertensive control for 12weeks. HDT, hypertensive trained for 8 and detrained for 4weeks. Data are mean±SEM of 90 cells in each group. ⁎, significantly different from NC8. #, significantly different from HC8. ¨ significantly different from NT8. +, significantly different from HC12. †, significantly different from NC12. ‡, significantly different from NDT. Journal of Molecular and Cellular Cardiology 2013 57, 119-128DOI: (10.1016/j.yjmcc.2013.01.013) Copyright © 2013 Elsevier Ltd Terms and Conditions

Fig. 6 Protein expression in the left ventricle. (A) Total phospholamban (PLBt). (B) Phosphorylated phospholamban at serine 16 (PLBser16). (C) Sarcoplasmic reticulum Ca2+ ATPase (SERCA2a). (D) Na+/Ca2+ exchanger (NCX). (E) PLBt/SERCA2a ratio. Targeted bands were normalized by GAPDH expression. NC8, normotensive control for 8weeks. NT8, normotensive trained for 8weeks. HC8, hypertensive control for 8weeks. HT8, hypertensive trained for 8weeks. NC12, normotensive control for 12weeks. NDT, normotensive trained for 8 and detrained for 4weeks. HC12, hypertensive control for 12weeks. HDT, hypertensive trained for 8 and detrained for 4weeks. Data are mean±SEM of 6–7 animals in each group. ⁎, significantly different from NC8. #, significantly different from HC8. Journal of Molecular and Cellular Cardiology 2013 57, 119-128DOI: (10.1016/j.yjmcc.2013.01.013) Copyright © 2013 Elsevier Ltd Terms and Conditions

Fig. 7 Gene expression in the left ventricle. (A) Atrial natriuretic factor (ANF). (B) Skeletal α-actin. (C) α/β-myosin heavy chain (MHC) ratio. Targeted genes were normalized by GAPDH mRNA. NC8, normotensive control for 8weeks. NT8, normotensive trained for 8weeks. HC8, hypertensive control for 8weeks. HT8, hypertensive trained for 8weeks. NC12, normotensive control for 12weeks. NDT, normotensive trained for 8 and detrained for 4weeks. HC12, hypertensive control for 12weeks. HDT, hypertensive trained for 8 and detrained for 4weeks. Data are mean±SEM of 5–6 animals in each group. #, significantly different from NC8. *, significantly different from HC8. †, significantly different from NC12. ‡, significantly different from NDT. Journal of Molecular and Cellular Cardiology 2013 57, 119-128DOI: (10.1016/j.yjmcc.2013.01.013) Copyright © 2013 Elsevier Ltd Terms and Conditions