Figure 1. Effect of the glucocorticoid (GC) cortisol at 1μ m on mac25/IGFBP-rP1 mRNA expression in cultures of Ob cells treated for 2, 6, 24, or 48 h.

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Figure 1. Effect of the glucocorticoid (GC) cortisol at 1μ m on mac25/IGFBP-rP1 mRNA expression in cultures of Ob cells treated for 2, 6, 24, or 48 h. Total RNA from control (−) or cortisol-treated (+) cultures was subjected to Northern blot analysis and hybridized with α-<sup>32</sup>P-labeled mac25/IGFBP-rP1 cDNA. The blot was stripped and rehybridized with labeled murine 18S cDNA. IGFBP-rP1 mRNA was visualized by autoradiography and is shown in the upper panel; 18S mRNA is shown below. Cortisol Enhances the Expression of mac25/Insulin-Like Growth Factor-Binding Protein-Related Protein-1 in Cultured Osteoblasts**This work was supported by NIDDK Grant DK-45227 and a scholarship (to R.C.P.) from Fundação Coordenação de Aperfeiçoa mento de Pessoal de Nível Superior (CAPES), Universidade de São Paulo (São Paulo, Brazil). Endocrinology. 1999;140(1):228-232. doi:10.1210/endo.140.1.6411 Endocrinology | Copyright © 1999 by The Endocrine Society 1