Antibiotics/Extracts

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Antibiotics/Extracts MODULATION OF ANTIBIOTIC RESISTANCE ON MULTIRESISTANT BACTERIAL STRAINS BY Croton campestris Edinardo F. F. MATIAS1, Karine Tako MARTINS1 1Acadêmico do Curso de Fisioterapia do Centro Universitário Dr. Leão Sampaio – UNILEÃO. 2Docente do Curso de Fisioterapia do Centro Universitário Dr. Leão Sampaio – UNILEÃO. ednardo@leaosampaio.edu.br; karine@leaosampaio.edu.br Palavras-Chaves: Palavra 1. Palavra 2. Palavra 3. RESULTS AND DISCUSSION INTRODUCTION The family Euphorbiaceae is made up of 317 genera and about 7500 species. The genus Croton, which has 700 species, is widely distributed in warm regions of the world1. The species Croton campestris A., popularly known as velame-do-campo, is a shrub originally from Brazil, occurring mainly in the southeast and northeast regions, and it is widely used in traditional medicine as a powerful depurative against scrophulosis, venereal diseases, tumors, skin diseases, rheumatism, ulcers of the uterus, diarrhea and arthritism2. This study shows the presence of various compounds such as: 1tannins phlobaphenes, 2flavones, 3flavonols, 4xanthones, 5chalcones, 6aurones, 7flavononols, 8catechins, 9flavonones, 10alkaloids and 11terpenes. The table under shows the MIC of the extracts and the potentiating effect in combination with antibiotics. The strain EC27 showed greater sensitivity to the extracts than did strain SA358, but when antibiotics were combined with extracts, the strain SA358 showed greater sensitivity. We found that HECC and the combination HECC-antibiotic was more effective. MECC – Methanol Extract Croton campestris; HECC – Hexane Extract Croton campestris; MIC values: µg/mL METABOLITICS EXTRATCS 1 2 3 4 5 6 7 8 9 10 11 MECC + - HECC The aim of this study was to do a phytochemical screening of the methanol and hexane extracts of Croton campestris A and to determine their potentiation of the antibiotic activity of aminoglycosides. EC 27 SA358 MIC Combined Antibiotics/Extracts MECC HECC Gentamicin 19 9 2,2 39 Kanamicin 157 317 78 4,5 Amicacin Neomicin 512 - ≥1024 256 MATERIALS AND METHOD Croton campestris The phytochemical tests to detect the presence of secundary metabolitics were performed according to the method described by Matos3. Determination of MIC and Activity modules4. Method of Distribution (BHI inoculum diluted in 10% [1:10]) Fill the plate in a numerical sense [100µL per cavity] Addition of 100µL of extract solution [1024µg/mL] in the 1st cavity Dilution steps from 1:1 to penultimate cavity [512- 8µg/mL] 12-24h incubation at 37ºC Blue = No growth Red = Presence of growth Addition of 20µL of resazurin. Reading after 1 hour. CONCLUSION This approach is not only for combinations of extracts; combinations between natural products or extracts and synthetic products or antibiotics are also possible5. The results obtained indicate that Croton campestris A. could serve as a source of plant-derived natural products that modify antibiotic resistance for use against multidrug-resistant bacteria, such as strains of Escherichia coli and Staphylococcus aureus acquired from the hospital and from the community. Method of Distribution (BHI inoculum diluted in 10% [1:10] + Extract in MIC/8) Fill the plate in a alphabetical sense [100µL per cavity] Addition of 100µL of antibiotic [5000µg/mL] in the 1st cavity Dilution steps from 1:1 to penultimate cavity [2500- 2,5µg/mL] 12-24h incubation at 37ºC Blue = No growth Red = Presence of growth Addition of 20µL of resazurin. Reading after 1 hour. REFERENCES 1HELUANI, C.S. et al. Three new diterpenoids based on novel sarcopetalene skeleton from Croton sarcopetalus. Journal of natural products, 63: 222-225, 2000. 2CRUZ, G.L. Dicionário das plantas úteis do Brasil. 2ª ed. Rio de Janeiro: Ed. EDEL. 1982. 3MATOS, F.J.A. Introdução à Fitoquímica Experimental. 2ª Ed. – Fortaleza: UFC. 1997. 4JAVADPOUR, M.M. et al. De novo antimicrobial peptides with low mammalian cell toxicity. Journal of medicinal chemistry, 39: 3107-3113, 1996. 5WAGNER, H.; ULRICH-MERZENICH, G. Synergy research: approaching a new generation of phytopharmaceuticals. Phytomedicine, 16: 97-110, 2009. Apoio: