Cell-based biosensors

Slides:



Advertisements
Similar presentations
Nanobiosensors Sara Huefner November 6, Outline Biosensor Background –What is a Biosensor? –Components of a Biosensor –Principles of Detection Biosensors.
Advertisements

Cell Biology Lecture 3. Function of Plasma Membrane Mechanical Support Cell Signaling Selective permeability Active transport Bulk Transport Metabolic.
NSF-ITR Meeting Miniaturized Sensors Air molecules aerosols Water molecules cells, viruses.
Introduction to Immunoassays
University of California, Irvine The Integrated Micro/Nano Summer Undergraduate Research Experience (IM-SURE) Single-Cell Platforms for Microbiomechanics.
Industrial and Medical Use of Enzymes. Biosensors.
Dr. Zahoor 1. Lecture 1: Homeostasis Lecture 4: Inter Cellular Communication & Signal Transduction 2.
Cells: Prokaryotic vs. Eukaryotic. Cells  Cells – basic unit of living organisms.  Level of organization: < cell < tissue < organ < organ system < organism.
Western Blotting.
WHAT IS A WESTERN BLOT?.
Microarray Technology
Enzyme-Linked Immunosorbent Assay [ELISA] BCH 462[practical] Lab#5.
School of Biomedical Engineering, Science & Health Systems V 1.0 SD [030814] NANOBIOSENSOR RESEARCH The Challenges PROGRAM OVERVIEWPROGRAM.
Cellular Communication Denise M. Green Denise Green.
Immunochemical methods
Biological Properties ISSUES TO ADDRESS... Biomaterials definition Different types of interaction between body and foreign material What are main characteristics.
School of Biomedical Engineering, Science & Health Systems V 2.0 SD [020204] Impedance Cardiography for Non-invasive.
Biomedical Electrodes, Sensors, and Transducers
Biosensors.
By Dominic Lanni. Part One Introduction  What are biosensors?  A device that uses a living organism or biological molecule to detect things  Usually.
Biosensor Development Aptasensor for a small organic molecule, TCA Peter Pfeiffer Bioengineering and Bioelectrochemistry Group University Rovira i Virgili.
School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020214] The sensor market is one of the fastest.
PTT 202 ORGANIC CHEMISTRY FOR BIOTECHNOLOGY Lecture 4: Electroanalytical Methods Zulkarnain Mohamed Idris Semester /2014.
Reporter: AGNES Purwidyantri Student ID no: D Biomedical Engineering Dept.
By Dominic Lanni. Part One Introduction  What are biosensors?  A device that uses a living organism or biological molecule to detect things  Usually.
Water toxicity detection through perfusion and monitoring of living cells on a microfluidic chip Fang Li, Ph.D. Department of Mechanical Engineering New.
TUTORIAL DISCUSSION – 1 PHSL- 215 Dr. Zahoor 1. Topics Lecture 1: Homeostasis Lecture 4: Inter Cellular Communication & Signal Transduction 2.
Use of Molecular Signatures for in situ Detection of Organisms and Genes They Express The Next Generation of in situ Biological and Chemical Sensors in.
Cagri Ozge Topal OSU ECEN 5060 Nanotechnology
General Approach in Investigation of Haemostasis
Biosensing What does it mean? How do you do it? Biosensor Definition Surface Chemistry Biology Physics Photonics etc Electrochemistry Microelectronics.
MONOCLONAL ANTIBODIES What are they? How are they produced? What potential uses?
CONTENTS INTRODUCTION COMPONENTS OF BIOSENSORS NANOBIOSENSORS TYPES OF NANOSENSORS AND THEIR APPLICATIONS ENVIRONMENTAL APPLICATIONS FUTURE APPLICATION.
Lecture 4 عضوية AMINES Amines are extremely important functional groups in medicinal chemistry and are present in many drugs. Amines are present in amino.
Lab# 5 Western Blot BCH 462[practical].
Multiplex analysis of cytokines Russell Garland KWS BioTest
BIOELECTRONICS 1 Dr. Eng. Hani Kasban Mahmoud
Haemagglutination assay
Introduction to Animal Tissue culture
Immunochromatography
BIO307- Bioengineering principles FALL 2016
Chemiluminecent microparticle)) immunoassay
Chemiluminecent microparticle MEIA)) immunoassay
Enzyme Linked Immunosorbent Assay
Immobilization Of Biomolecules On Biosensors
Cagri Ozge Topal OSU ECEN 5060 Nanotechnology
Technological Principles of Medical Instrumentation
ELISA.
BioSignal Processing.
Biosensor 서울대학교 화학생물공학부 박 태 현.
Vlp construction The formation of virus-like particles (VLPs) is a result of self- assembly of one or several types of viral structural proteins, such.
Enzyme-Linked Immunosorbent Assay [ELISA]
Preparation of Surface for Biomolecule Immobilization
Vlp construction The formation of virus-like particles (VLPs) is a result of self-assembly of one or several types of viral structural proteins, such as.
Immunochromatography
Proteon Scientists from Creative Biolabs are willing to provide custom antibody affinity measurement using ProteOn system. The system is a surface plasmon.
Cell Communication Involves transduction of stimulatory or inhibitory signals from other cells, organisms or the environment Believed to have evolved in.
Cell Signaling (Lecture 1)
Microfluidic Cell Culture Analog Devices to Mimic Animal Exposures to Toxins and Drugs Our group has developed microfluidic in vitro devices that mimic.
Figure 2 Biosensor-based diagnosis of UTI
FROM BLOOD TO CELLS Pancreas and liver regulate blood glucose in a feedback loop.
In multicellular organisms
BIOSENSOR.
Surface Plasmon Resonance (SPR) and Quartz Crystal Microbalance (QCM)
Zihua Zeng, Ching-Hsuan Tung, Youli Zu 
Immunochemical methods
Experimental Systems and Methods
BIO307- Bioengineering principles SPRING 2019
Practical of Histopathology
Volume 105, Issue 2, Pages (July 2013)
Presentation transcript:

Cell-based biosensors LECTURE OF SUBJECT : Dr. sharafaldin Al-musawi College of Biotecholgy LECTURE: 7 SUBJECT: Biosensors & Biochips LEVEL: 4 Cell-based biosensors

Cell-Based Biosensor

Cell-based biosensors Cell-based biosensors utilize the principles of cell-based assays by employing living cells for detection of different analytes from environment, food, clinical, or other sources. CBBs incorporate both prokaryotic (bacteria) and eukaryotic (yeast, invertebrate and vertebrate) cells. To create CBB devices, living cells are directly integrated onto the biosensor platform. Usually use in biomedical fields. Utilized to provide information on how a large variety of factors can affect many different types of cells.

Heavy metal whole-cell biosensors 

Cell-based QCM biosensor By attachment and spreading of mammalian cells onto the solid electrode of the quartz sensor we can monitoring changes in the beating rate of cardiac myocytes due to the addition of drugs we propose a cell-based QCM biosensor. To demonstrate this the QCM method was used to monitor the mechanical contraction of cardiac myocytes.

Cell-based biosensor for disease detection Impedance biosensor for measurement of immunoreaction coupled with red blood cell (RBC) amplification. (A) gold electrode surface modification by polyclonal anti-N1 antibody. (B) H5N1virions binding and detection. (C) RBC amplification. RBCs were used as bio labels to attach to captured H5N1 to amplify impedance signal by amplification of the antibody–antigen reaction.

Cell-based biosensor for toxin detection The main advantage of using cell based biosensor for probing biotoxins and toxic agents is that cell based biosensor respond to the toxic exposures in the manner related to actual physiologic responses of the vulnerable subjects. The results obtained from cell based biosensor are based on the toxin-cell interactions, and therefore, reveal functional information (such as mode of action, toxic potency, bioavailability, target tissue or organ, etc.) about the toxin.

The sensors report the cellular responses upon exposures to toxins and the resulting cellular signals are transduced by secondary transducers generating optical or electrical signals outputs followed by appropriate read-outs.

Cell-based biosensor for detection the presence or absence of specific cell By this cell-based biosensor we can to infer the presence or absence of specific cellular environment. When cells cover the electrode the measured impedance changes because the cell membranes block the current flow.

Cell-based biosensor for detection of cancer cells A novel lectin-based suspension-cell biosensor for label-free determination of binding kinetics of protein–carbohydrate interactions on cancer cell surfaces using QCM is described. This cell-biosensor facilitates evaluation of glycosylation in real time on suspension cancer cell surfaces, where binding events take place.

Cell-based biosensor for detection of cancer cells The figure shows the results of the invasion properties of different prostate cancer cells. Prostate endothelial cells were inoculated first and spread on the electrode for 24 hours which increased the impedance to around 9,000 Ohms. Then different cancer cells were added and the invasion of cancer cells caused the impedance to decrease to 8,000 Ohms. The impedance after inoculating prostate cancer cells immediately decreased, showing these cells are the most invasive.