ASF epidemiological situation in Latvia – 2017

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Presentation transcript:

ASF epidemiological situation in Latvia – 2017 Mārtiņš Seržants Food and Veterinary service of Latvia GF TAD`s Chisinau, 20.09.2017.

ASF cases in 2017 (on 14.09.2017) 2017 (14.19.) PCR ELISA Wild boar Tested Hunted Found dead 9080 8336 744 8503 Wild boar cases Hunted 989 403 586 767 222

Disposal of wild boar carcass

Estimation of wild boar population in Latvia Financial support in combination with informative campaigns rise hunting activity and wild boar female hunting as well. Since the year 2014 estimated number of wild boar reduce 3x.

Feeding and baiting Feeding is forbidden Baiting is important tool for hunting (attraction)

Feeding or baiting don't play important role in mild winter or under certain condition...

Wild boar biology Reproduction of WB could be fastest than virus inactivation in environment.

Outbreaks in 2017 (on 17.09.2017) 4539 8521 5818 Outbreak Date Affected pigs No 1 14.01 4539 No 2 7.02. 8521 No 3 12.07. 3 No 4 14.07. 5818 No 5 3.09. 9 No 6 13.09. 23

Logistic and disposal of carcasses in the case of large outbreaks Rendering plants are overloaded during the outbreaks! Alternative disposal methods could be a solution.

Laboratory capability and diagnosis Preparation of tissue suspension and blood sample to testing is a time-consuming procedures GOOD SAMPLE QUALITY = RAPID & CORRECT DIAGNOSIS

ASF diagnostic scheme ASFV genome RT-PCR (blood, tissue samples and bone marrow) Virus genome Negative Virus genome Positive Negative Positive Antibody – ELISA (serum samples) Confirmatory test IPT (serum and bone marrow exudate) Ab Negative Ab Positive Sequencing of material

ASF Virus detection methods SOP/CISA/ASF/PCR/3/, 2014, “Detection of African swine fever virus (ASFV) by real- time PCR using Universal probe library (UPL)”. Fernández-Pinero, J. (2013). Molecular diagnosis of African swine fever (ASF) by a new real-time PCR using Universal Probe Library (UPL). Transbound Emerg. Dis., 60: 48-58 SOP/CISA/ASF/GENOTYPING/1, Rev.2013, “Detection of ASF virus isolates”. Gallardo et al., 2009, Enhanced discrimination of African swine fever virus isolates through nucleotidee sequencing of the p54, p72, and pB602L (CVR) genes. Virus genes, Feb: 38(1):85-95

Asf serological diagnostic methods Blocking ELISA, INGEZIM PPA COMPAC (INGENASA) (1) SOP/CISA/ASF/IPT/1, Rev.2014-2 “Indirect Immunoperoxidase techniques for ASF antibody detection”. (p72, genotype 1) (2) SOP/CISA/ASF/IB/1, Rev.2013 “The detection of antibodies against ASF by immunoblotting”. Detection of antibodies to 8 main viral proteins - IP12.5, IP23.5, IP25, IP25.5, IP30, IP31, IP34, IP35 (3) Indirect ELISA, ID Screening ASF (ID.VET) (4) Indirect ELISA, ID Confirmatory ASF (ID.VET)

Laboratory capacity Capacity of the national African Swine Fever laboratory is (on a weekly basis): Antibodies (ELISA): 2000 tests Antibodies (IPT): 750 tests Virus genome (PCR): 2000 tests in the case of emergency x3

Risk comunication How to make everybody envolved? Legislation (National and European) Contingency plan Funding should be foreseen for activities carried out Regular training and awareness campaigns Communication and collaboration between institutions (also in peace time) Veterinarians , hunters and stake holders

Thank you for your attention!