glomerular homogenate

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glomerular homogenate Supplementary Figure １ A C Rat glomerular homogenate Conditions and specific primers for real-time PCR methods Target mRNA Human GLP-1 receptor Rat GLP-1 receptor Preincubation 95ºC, 30 s PCR 92ºC, 1 s 62ºC, 10 s 72ºC, 15 s Sense primer 5’-GTT CCC CTG CTG TTT GTT GT-3’ 5'-ATC CAC CTG AAC CTG TTT GC-3' Antisense primer 5’-CTT GGC AAG TCT GCA TTT GA-3’ 5'-GCA GTA TTG CAT GAG CAG GA-3' Product size 228 bp 174 bp 200 100 Rat GLP-1R 174bp 50 B NHMCs 300 200 Human GLP-1R 228bp 100 50 Supplementary Fig. 1 The expression of GLP-1 receptor mRNA in rat glomerular homogenates (a) and normal human mesangial cells (NHMCs) (b) used in the experiments were confirmed by real-time PCR analysis. Conditions and specifics are indicated in C.