LIQUID CHROMATOGRAPHY High PERFORMANCE LIQUID CHROMATOGRAPHY 3000 PLUS

Analytical Technologies is synonymous for offering technologies for doing analysis and is the fastest growing global brand having presence in at least 96 countries across the globe. Analytical Technologies Limited is an ISO:9001 Certified Company engaged in Designing, Manufacturing, Sales, Marketing, Distribution & Providing Services for the Analytical, Chromatography, Spectroscopy, Biotechnology, Biomedical, Clinical Diagnostics, Material Science & General Laboratory Equipment. Analytical Technologies, India has across the Country operations with at least 4 Regional Offices, 6 Branch Offices & Service Center. Analytical Technologies also provides Accessories, Consumables, Services and trainings.

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INTRODUCTION High performance liquid chromatography (HPLC) is a form of High-pressure chromatography that uses a pump to control the speed at which the mobile phase passes through the stationary phase. High performance liquid chromatography systems generally consist of a  Quaternary pump, Auto sampler, Column oven, UV-Visible detector, Refractive index detector, Diode array detector and operate at pressures of ~9,000 psi.

PUMP - 3000 PLUS Analytical Technologies 3000 Plus HPLC with quaternary pump. Max. Pressure: 9000 psi Flow Rate: 0.0001 - 10.000 ml/min (increment 0.001 ml/min.) Flow Accuracy:± 0.01% (1.000 ml / min) Flow Precision:± 0.1 % RSD Composition Precision - ≤ 0.1% RSD Concentration accuracy - ± 0.5% or better Gradient reproducibility - ≤ 0.2% SD

Quaternary System with Auto sampler- 3000 Plus Quaternary system possesses the advantage of great flexibility, and it can further be automatized by combing our auto sampler. This combination could fulfill the complex requirements from pharmacy, agricultural & farming fields. With the control from Chromatography workstation, it realized full automatic analysis, report, system flush, shut down and then raised up the efficiency. environmental, food safety, petrochemical engineering.

Auto sampler- 3000 Plus Autosampler delivers high injection accuracy of 0.3% RSD with advanced control algorithm. Multi injection mode have three sampling mode including full loop, partial loop and micro pick up injection. For low cross contamination, special design of needle washing assembly could clean interior and outside of the sampling needle separately. Cross contamination - <0.1% Injection Volume – Programmable from 0µl – 99.99 µl Injection precision : Full loop injection <0.2% RSD Partial loop-fill <0.5% RSD µl-pick-up <1.0% RSD Carry Over <0.003% RSD with standard wash Typical <0.001% RSD with extra wash Vials : 2x 48 (1.5ml) Sample capacity – Micro Plates : 192 Vials : 120 (2ml)

Working principle of Autosampler

Column Oven – 3000 plus Temperature range - 10ºC to 100ºC Temperature accuracy - ± 1.0ºC Temperature stability - ± 1.0ºC Temperature precision - ± 1.0ºC Column Length – 300mm, up to two columns Option – Peltier Power Consumption – 145W

UV- Vis Detector-3000 Plus As support by unique high speed signal processing technology, UV-VIS detector realized 100 Hz sampling rate, which make the detection of narrow peak with width lower than 5s possible. Its wavelength range : 190-800 nm with accuracy of +1 nm This high sampling rate also helps to record the chromatography peak accurately and get the best resolution.

Diode Array Detector-3000 Plus 3000plus has a 1024 diode array sensor with pixel resolution of 0.6nm. Combine with high quality optical grating, Record full high resolution spectrum while chromatograph recording. Its wavelength range : 190-800 nm with accuracy of +1 nm This high sampling rate also helps to record the chromatography peak accurately and get the best resolution.

Refractive Index Detector- 3000 Plus Refractive Index Detector with Deflection type measuring method. Refractive index range – 1.00 to 1.75 Measuring range – 0.125 to 256 µRIU Temperature range – 0-50ºC Flow rate – 0.2-3.0 ml/min Auto zero – Full auto zero Auto zero range – All range

Chromatography WorkStation Analchrome® workstation furthers the capability of Analytical Technologies High Performance Liquid Chromatography- 3000 PLUS system with Analysis solution research and development, instrument control, data acquisition and processing, report generation, and traceable record management with versatile extensibilities.

Analchrom chromatography workstation in a way to comply with the requirements of the 21 CFR Part 11 directive of the FDA. This ensures its suitability for use in regulated environments. Audit Trail of whole system, chromatograms, calibrations and sequence. Audit Trails are part of corresponding files. Detailed logs and histories of modifications enable users to maintain an audit trail. The station documents all parameters describing the conditions and methods of data processing for the user. This allows for easy access to a complete profile of information regarding any prior modification's performance.

Direct instrument control plus intuitive user interface give user the simplicity of operating the instrument from software. Simple method editing interface and powerful data processing with more than twenty integral functions.

Various calibration procedures and customizable report editor.

Application and solutions

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