ABX Pentra DF 120 Flexible hematology platform For internal use only Prepared by L.Castellane

Main features analysis Methods and technologies Pentra ML ABX Pentra DF 120 Main features analysis Methods and technologies Pentra ML Prepared by L.Castellane

Optional slide maker and stainer Main features Rack loader Reagent compartment Technical software Processed tubes Stat Sample Bay ABX Pentra ML Optional slide maker and stainer Prepared by L.Castellane

IMmature Lymphocyte, Monocyte and Granulocyte Main features CBC, DIFF IMmature Lymphocyte, Monocyte and Granulocyte Optional and integrated slide maker and stainer Expert Validation Station Prepared by L.Castellane

Wbc, Rbc, Hgb, Hct, Mcv, Mch, Mchc, Rdw, Plt, Mpv, Pct, Pdw Main features 32 Parameters CBC (12 parameters) Wbc, Rbc, Hgb, Hct, Mcv, Mch, Mchc, Rdw, Plt, Mpv, Pct, Pdw Differential count (12+20 parameters) Lym, Mon, Neu, Eos, Baso, Aly, Lic (% and #) IML, IMM, IMG (% and #) Throughput (12O samples per hour) Prepared by L.Castellane

5 reagents Unique blood control ABX Difftrol - 3 levels ABX Diluent Main features 5 reagents ABX Diluent ABX Basolyse ABX Leucodiff ABX Alphalyse or ABX Lysebio (Cyanide free) ABX Cleaner Unique blood control ABX Difftrol - 3 levels Target values on disk, multi-language package insert ABX reagents and controls are bar-coded and CE marked Batch number, level detection, small reagent volumes, waste level sensor Prepared by L.Castellane

External and internal bar-code reader Main features 3 types of analyses (with random access) CBC DIFF SPS External and internal bar-code reader Prepared by L.Castellane

Main features analysis Methods and technologies Pentra ML ABX Pentra DF 120 Main features analysis Methods and technologies Pentra ML CBC principles 5 Diff – Double matrix Prepared by L.Castellane

Methods & technologies RBC/PLT - Impedance method HGB - Cyanmethemoglobin or cyanide free WBC - (Triangulation) -> WBC channel (impedance) -> BASO channel (Specific lyse + impedance) -> 5Diff channel (optical) Prepared by L.Castellane

Methods & technologies 5Diff Principles  1. Cytochemistry Lysing of red blood cells Staining of nuclei and cytoplasmic granules of leukocytes Fixing the leukocytes in their original state (48 hours post-draw stability) 2. Double Hydrodynamic Sequential System (DHSS) Flow cytometer with hydrodynamic focusing before and after the micro-aperture 3. Impedance method Measurement of the cell volume (“X” axis)    4. Light absorbance Internal structure (“Y” axis) Prepared by L.Castellane

Methods & technologies Double Diff Matrix Eosinophils Large Immature Cells Neutrophils IMmature Granulocytic line Lymphocytes IMmature Monocytic line Monocytes IMmature Lymphocytic line Atypical LYmphocytes Prepared by L.Castellane

Methods & technologies IMmature Granulocytes Myeloblast IMG Promyelocyte Myelocyte Metamyelocyte IMG % and # Identification of Granulocytic cell line count of the overall myelemia Prepared by L.Castellane

Methods & technologies IMmature Monocyte Monoblast Promonocyte IMM IMM % and # Identification of Monocytic cell line Count of the precursors of the monocytic line Prepared by L.Castellane

Methods & technologies IMmature Lymphocyte Lymphoblast Prolymphocyte IML IML % and # Identification of Lymphocytic cell line IML and ALY include all abnormal lymphocytic populations Prepared by L.Castellane

Methods & technologies Routine identification and quantification of 3 immature cell lines involved in leucopoiesis: Granulocytic line Monocytic line Lymphocytic line -> Reduction of the number of slide examinations -> Diagnostic and follow-up tool for rapid decision-making Prepared by L.Castellane

Methods & technologies 1- Priority alarms 2- Separation alarms ALY: (Atypical Lymphocytes) Atypical Lymphocytes Plasmocytes, hairy Cells... Ne: (Neutro-eosino) Hyper-segmented neutrophils Hypo-granular eosinophils... Mn: (Mono-neutro) Immature granulocytes Band cells... LIC: (Large Immature Cells) Blasts, myelocytes Promyelocytes Metamyelocytes... Nl: (Neutro-lympho) Hypo-segmented neutrophils Sezary cells, hairy cells... Prepared by L.Castellane

Methods & technologies 3- Zone alarms No: (Noise) Platelet aggregates, NRBCs Lysis resistant RBCs Ln: (Left-neutro) Cellular debris Ll, Ll1: (Left-lympho) Platelet aggregates, NRBCs Rn: (Right-neutro) Immature granulocytes Rm: (Right-mono) Large monocytes, blasts Prepared by L.Castellane

Methods & technologies CHRONIC MYELOID LEUKEMIA Pentra DF 120 result: NEU 25.4 % EOS 1.8 % LYM 5.1 % MON 8.6 % BAS 0.1 % IMG 49.3 % IMM 9.2 % IML 0.5 % ALY 1.3 % Prepared by L.Castellane

Methods & technologies ACUTE MONOBLASTIC & MONOCYTIC LEUKEMIA Pentra DF 120 result: NEU 11.1 % EOS 0.5 % LYM 9.3 % MON 47.9 % BAS 0.1 % IMG 4.1 % IMM 26.1 % IML 0.9 % ALY 1.1 % Prepared by L.Castellane

Methods & technologies T-CELL PROLYMPHOCYTIC LEUKEMIA Pentra DF 120 result: NEU 12.1 % EOS 0.4 % LYM 6.7 % MON 12.8 % BAS 0.4 % IMG 0.4 % IMM 2.0 % IML 7.4 % ALY 57.8 % Prepared by L.Castellane

Main features analysis Methods and technologies Pentra ML ABX Pentra DF 120 Main features analysis Methods and technologies Pentra ML Specs & functionality Prepared by L.Castellane

17 inch flat colour screen Windows XP CD-ROM burner Automatic rerun Specs & functionality Dedicated PC 17 inch flat colour screen Windows XP CD-ROM burner Automatic rerun Reflex testing Pre-diluted mode Cytology library Onboard user manual Prepared by L.Castellane

Mono or bi-directional connection: Specs & functionality Mono or bi-directional connection: ASTM format Transfer of Demographic data (blood type - man, woman, child) Tests requested (CBC, CBC/DIFF, SPS) Up to 3 ABX Hematology analyzers (Pentra range) can be connected Query mode Download capability Prepared by L.Castellane

User defined rules applied when : receiving the worklist, Specs & functionality User defined rules applied when : receiving the worklist, a sample has been analyzed. _ Rerun capability _ Reflex testing _ Automatic validation _ Messages, comments…. Bio-technical validation with : Patient history Delta check Result edition Manual entry Recalculation capability Prepared by L.Castellane

Bio-technical validation with : Result screens Bio-technical validation with : Patient report, run, re-run, patient history, graphs, alarms. All displayed on the same screen. Delta check Result edition Manual entry Recalculation capability 1- Title bar 2- Patient and Graphics area 3- Messages 4- Patient Comments 5- Technical Comments 6- Extended Diff 7- Result area 8- Report 9- Runs Prepared by L.Castellane

Hematovision IV included A cell cytology library Specs & functionality Hematovision IV included A cell cytology library Prepared by L.Castellane

ABX Pentra 120 series Prepared by L.Castellane

ABX Pentra 120 series Prepared by L.Castellane

Thank you Prepared by L.Castellane