iGEM Meeting – 19/04/17: SELEX & Riboswitches

SELEX Systematic Evolution of Ligands by EXponential enrichment Generation of RNA (or DNA) aptamers by in vitro selection Random DNA oligos RNA oligos In vitro transcription Incubate library with ligand In vitro transcription Remove bound aptamers Remove unbound aptamers RT-PCR Incubate library with undesired ligands In vitro transcription RT-PCR

Aptamer to Riboswitch In vivo riboswitch selection Identification of RNA aptamers with riboswitching properties. DOI: http://doi.org/10.1016/j.ymeth.2015.12.001

Modelling for in vivo Selection Determine riboswitches which fold correctly Uses free energy calculations Automated physics-based design of synthetic riboswitches from diverse RNA aptamers. DOI: https://doi.org/10.1093/nar/gkv1289

Ribozymes In vitro selection of allosteric ribozymes that sense the bacterial second messenger c-di-GMP. DOI: 10.1007/978-1-62703-755-6_15.

Toehold Switches Toehold Switches: De-Novo-Designed Regulators of Gene Expression. DOI: http://dx.doi.org/10.1016/j.cell.2014.10.002

Dual-Colour Riboswitch Riboswitch-Based Reversible Dual Color Sensor. DOI: 10.1021/acssynbio.6b00199