Influence of conjugated linoleic acid (CLA) supplementation on Haptoglobin and Leptin during the lactation period in dairy cattle Amani Al-Dawood Supervised by Prof. Dr. Dr. Sauerwein

Objectives To assess the potential effects of long-term CLA supplementation during the lactation period on: 1 - Haptoglobin as inflammation marker. 2 - Adipokines (leptin).

Experiment 1….Results…Hp…All animals Group p=0.646 Sample p=0.000 n=49 animals Control 50g CLA Calving Stop CLA feeding ≥ 0.15 mg/ml < 0.15 mg/ml Control 108 180 50 g CLA 93 194 100 g CLA 105 192 100g CLA Mean Hp concentration (mg/mL) P=0.039 Days relative to parturition Figure 1: Mean (±SEM) Haptoglobin concentration (mg/mL) by feeding dairy cattle on two different concentrations of CLA and control treatment in relative to parturition.

Control 50 g CLA 100 g CLA Muscle absces Mastitis Endometritis Mean Hp concentration (mg/mL) Endometritis Metritis 50 g CLA Endometritis Mastitis Mean Hp concentration (mg/mL) Muscle absces 100 g CLA Absces Mean Hp concentration (mg/mL) Mastitis Mastitis Days relative to parturition

Mean Hp concentration (mg/mL) Experiment 2….Results…Hp n=25 animals ns Mean Hp concentration (mg/mL) Days relative to parturition Figure 2: Mean (±SEM) Hp concentration (mg/mL) in control group and CLA treatment. ns= not significant

Day 1 Day 42 con Day 42 CLA Day 105 con Day 105 CLA Mean Hp concentration (mg/mL) Day 42 con Day 42 CLA Mean Hp concentration (mg/mL) Mean Hp concentration (mg/mL) Day 105 con Day 105 CLA Mean Hp concentration (mg/mL) Mean Hp concentration (mg/mL) Days relative to parturition Days relative to parturition

leptin concentration (ng/mL) Days relative to parturition Experiment 1…Results…Leptin…All animals n=49 animals Control Calving Stop CLA feeding Group p=0.012 Con with 50 p=0.021 50 with 100 p=0.038 Sample p=0.000 50g CLA 100g CLA leptin concentration (ng/mL) Days relative to parturition Figure 3: Mean (±SEM) leptin concentration (ng/mL) by feeding dairy cattle on two different concentrations of CLA and control treatment in relative to parturition.

Thank you for your attention

J. Anim. Sci. 2007. 85:1923–1932