Mike Jepson and Paul Humphreys MTP INNOVATIONS Ltd Mike Jepson and Paul Humphreys

DIFFX Disinfection Sachet (Features & Benefits) Powerful disinfection system Fully enclosed soluble sachet Non-corrosive Environmentally friendly 100% biodegradable Microfibre friendly Active peracetic acid Sporicidal Kills MRSA & Norovirus Kills Mycobacteria (TB) and spores of C.Diff in ALL conditions

DIFFX Disinfection Sachet SACHET TESTS EN1276 – Suspension Bacterial test EN1650 – Suspension Fungal test EN13697 – Surface Disinfection Test (adapted for Spores) EN13704 – Suspension Sporicidal Test EN14348 – Suspension Mycobacterium test EN14476 – Suspension Viral Test (also modified for Norovirus) Hospital Infection Research Laboratory Verification by Tina Bradley

The National Hospital Trial 8 week trial, standard cleaning using cloths and mops to test the anti-microbial effectiveness of DIFFX. 10 TVC’s measured at critical points around a bed and a WC. TVC’s 1 hour post cleaning reduced by 40% using standard cleaning and 90% using DIFFX Used mops and cleaners gloves contamination was significantly reduced using DIFFX, compared to standard cleaning Conclusion was DIFFX was novel, safe, cost effective, easy to use and effective PAA based product for hospital cleaning and disinfection June 2012, further 8 week trial evaluating user acceptability with the new refined, reduced odour DIFFX Conclusion was that the new product was as good as the previous trial regarding cleaning and disinfection. Domestic teams and ward staff were very happy with the product and the new low odour was more than acceptable

DIFFX/Chlorine Product Comparison 8 WEEK STUDY

Introduction A combination of on-site sampling and laboratory studies were used to compare the performance of DIFFX and Chlorine Dioxide The aim of the study was to determine if DIFFX was as good or better at sanitising floors and high contact surfaces than the Chlorine Dioxide

The Study Three investigations performed: Comparison of before and after TVCs on a range of high contact surfaces and floors within two wards Ward A Ward B Bench top wiping of bench surfaces contaminated with C. difficile spores. Bench top wiping of bench surfaces contaminated with Staphylococcus epidermidis.

Hospital Study Crossover design 4 weeks on both wards 10 surfaces per ward TSA contact plates with neutraliser employed before and immediately after cleaning Neither product expected to have residual activity

Study Design Study Protocol/Weeks Weeks 1 2 3 4 5 6 7 8 Ward 1 Standard Diff-X Ward 2 Sampling Locations Blue stands behind the beds Radiator tops Service panel behind bed Door handle Windowsill Patient table Floor - middle of ward Floor - between the beds Floor - side of bed Floor -main entrance

Results DIFFX consistently reduced TVCs Chlorine product results more variable Rapid recontamination of surfaces evident

Conclusions No obvious difference between 1:3 DIFFX and Chlorine based product in laboratory tests However, in ward based trials DIFFX performed better than the Chlorine based product Poor performance of Chlorine product likely to be due to how it is being used and the levels of organic contamination it is experiencing DIFFX appears to be more robust and less likely to be affected by poor application and use DIFFX at 1:1 = 100% removal of spores over 0.75m2

Product Inter-comparison: DIFFX vs Chlorclean (1000 ppm available Chlorine) and Haztabs (10,000 ppm available Chlorine).