phosphate- buffered saline pH 7.4

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Presentation transcript:

phosphate- buffered saline pH 7.4 Table 1. Intrinsic viscosity, molecular weight, Mark-Houwink parameters dates of various studies released with dextrans at different temperatures T (°C) Solvent M (Da) [η] (cm3/g) a kM-H (cm3/g) σ2 Reference 25 Water 20000 - 100000 - 0.5000 0.0974 [27] f 20 10000 -500000 0.4300 0.0443 [28] f Formamide 2000 - 32000 0.4900 0.0165 [29] f 0.5M NaOH 500000 - 2000000 0.4780 0.0132 [30] f 30 0.1M NaNO3 / 0.025M K2HPO4 0.05M Na2SO4 97000 – 1750000 0.3440 0.2780 0.0476 0.1130 [31] g 0.2M NaNO3 40000 - 590000 0.3900 0.0485 0.8800 [32] a 0.4M NaCH3COOH / 0.4M CH3COOH 9500 - 2000000 11.0 – 140.0 0.4800 0.0138 [33] b 17.8 - 48.2 0.5330 0.07337 0.9817 31 17.3 - 45.9 0.5190 0.08268 0.9804 34 29500* - 191500* 17.1 - 44.8 0.5150 0.08474 0.9783 [34] f 40 16.6 - 42.9 0.5080 0.08600 0.9875 43 16.3 - 41.6 0.09462 0.9854 25.1 - 68.3 0.1050 0.9996 23.9 - 63.4 0.5250 0.1070 0.9995 35 Dimethyl- sulfoxide 21.9 - 57.8 0.2085 0.9991 [35] e 21.0 - 55.1 0.5170 0.1040 0.9994 45 18.2 - 47.7 0.5140 0.0919 17.8 - 50.9 0.5620 0.0551 0.9895 15.4 - 50.9 0.5490 0.0548 0.9691 Ethylene-glycol 13.6 - 37.6 0.5430 0.0511 0.9871 [36] b 12.6 - 34,7 0.5400 0.0486 0.9893 11,7 - 31.8 0.5340 0.9879 Water - NaOH 0.5M 9000 - 506000 8.6 - 53.1 0.4363 0.1900 0.9662 [1] a 40000 - 500000 21.0 - 50.0 0.3311 0.6066 [37] b 9000 -600000 12.0 - 60.0 0.3775 0.3685 0.9922 [3] d phosphate- buffered saline pH 7.4 19500 - 500000 10.97 - 50.50 0.4500 0.1361 0.9863 [8] c 12000 - 520000 11.8 - 51.6 0.3873 0.3197 0.9975 [16] b 65 39000 20.0 16.5 15.3 [38] b 37 42973 - 1988000 17.8 - 76.5 0.3888 0.2628 0.9887 [5] b 1270 – 676000 180 – 158000 226000 – 1360000 1907000 - 5900000 2.8 - 70.8 1.2 – 35.0 46.3 – 91.8 73.6 – 104.5 0.5060 0.5120 0.4250 0.2730 0.0901 0.0832 0.2297 1.4300 0.9870 0.9950 0.9170 0.8260 [39] b With permission: a- American Chemical Society. b- Elsevier Science B.V. c- Biophysical Society, Elsevier Science B.V. d- Amersham Biosciences AB. e- Springer-Verlag. f- John Wiley and Sons. g- Unilever Research. * Dates calculated from number average molecular weight. Martin Alberto Masuelli et al. Dextrans in Aqueous Solution. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect. Journal of Polymer and Biopolymer Physics Chemistry, 2013, Vol. 1, No. 1, 13-21. doi:10.12691/jpbpc-1-1-3 © The Author(s) 2013. Published by Science and Education Publishing.