PCR conditions: 94ºC for 15 min

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Presentation transcript:

PCR conditions: 94ºC for 15 min Subject DNA DS10 Subject DNA DS10 Negative control DS10 Subject DNA DS11 Subject DNA DS11 Negative control DS11 Subject DNA DS12 Subject DNA DS12 Negative control DS12 Subject DNA Subject DNA Negative control DS13a21 DS13a21 DS13a21 Subject DNA Subject DNA Negative control Subject DNA DS142 Subject DNA DS142 Negative control DS142 Subject DNA DS15 Subject DNA DS15 Negative control DS15 Subject DNA DS16 Subject DNA DS16 Negative control DS16 DS13b DS13b DS13b Subject DNA DS17 Subject DNA DS17 Negative control DS17 Subject DNA DS20 Subject DNA DS20 Negative control DS20 Subject DNA DS21 Subject DNA DS21 Negative control DS21 Subject DNA DS22 Subject DNA DS22 Negative control DS22 Subject DNA DS23 Subject DNA DS23 Negative control DS23 Subject DNA DS24 Subject DNA DS24 Negative control DS24 Subject DNA DS25 Subject DNA DS25 Negative control DS25 Subject DNA DS26 Subject DNA DS26 Negative control DS26 Subject DNA DS29 Subject DNA DS29 Negative control DS29 Subject DNA DS30 Subject DNA DS30 Negative control DS30 Subject DNA DS31 Subject DNA DS31 Negative control DS31 Subject DNA DS32 Subject DNA DS32 Negative control DS32 Subject DNA DS33 Subject DNA DS33 Negative control DS33 Subject DNA DS34 Subject DNA DS34 Negative control DS34 Subject DNA DS35 Subject DNA DS35 Negative control DS35 Subject DNA DS36 Subject DNA DS36 Negative control DS36 PCR conditions: 94ºC for 15 min 40 cycles of following: 94ºC for 20 sec 58ºC for 30 sec1, 2 72ºC for 60 sec 72ºC for 3 min 1PCR for DS13a2 is conducted at a primer annealing temperature of 53ºC. 2PCR for DS14 is conducted at a primer annealing temperature of 62ºC. Supplementary Figure - PRNP PCRs in 96-well format.