High levels of whole-body energy expenditure are associated with a lower coupling of skeletal muscle mitochondria in C57Bl/6 mice Sjoerd A.A. van den.

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High levels of whole-body energy expenditure are associated with a lower coupling of skeletal muscle mitochondria in C57Bl/6 mice Sjoerd A.A. van den Berg, Miranda Nabben, Silvia Bijland, Peter J. Voshol, Jan Bert van Klinken, Louis M. Havekes, Johannes A. Romijn, Joris Hoeks, Matthijs K. Hesselink, Patrick Schrauwen, Ko Willems van Dijk Metabolism - Clinical and Experimental Volume 59, Issue 11, Pages 1612-1618 (November 2010) DOI: 10.1016/j.metabol.2010.03.008 Copyright © 2010 Elsevier Inc. Terms and Conditions

Fig. 1 Animals were divided into 2 groups based upon negative or positive residual EE, after correction of total EE for body mass. A, Regression analysis of body mass and EE. B, Residual plot of regression analysis body mass and EE. Metabolism - Clinical and Experimental 2010 59, 1612-1618DOI: (10.1016/j.metabol.2010.03.008) Copyright © 2010 Elsevier Inc. Terms and Conditions

Fig. 2 In vivo metabolic characteristics of the negative (white bars) and positive (black bars) residual EE groups. DEE indicates diurnal EE; NEE, nocturnal EE; DFA, diurnal Fox; NFA, nocturnal Fox; DCO, diurnal CHox; and NCO, nocturnal CHox. Graph represents means ± SD; *P < .05. Metabolism - Clinical and Experimental 2010 59, 1612-1618DOI: (10.1016/j.metabol.2010.03.008) Copyright © 2010 Elsevier Inc. Terms and Conditions

Fig. 3 Respirometry analysis of hind limb–derived mitochondria of the negative (white bars) and positive (black bars) residual EE groups. State 3 and 4o respiration levels were measured using pyruvate (left) and palmitoyl-CoA (right) substrates and expressed as percentage of maximally uncoupled respiration. Graph represents means ± SD; *P < .05. Metabolism - Clinical and Experimental 2010 59, 1612-1618DOI: (10.1016/j.metabol.2010.03.008) Copyright © 2010 Elsevier Inc. Terms and Conditions