Rapid diagnosis and genotyping of Leishmania isolates from cutaneous and visceral leishmaniasis by microcapillary cultivation and polymerase chain reaction–restriction.

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Rapid diagnosis and genotyping of Leishmania isolates from cutaneous and visceral leishmaniasis by microcapillary cultivation and polymerase chain reaction–restriction fragment length polymorphism of miniexon region Mehmet S. Serin, Kenan Daglioglu, Melahat Bagirova, Adil Allahverdiyev, Soner Uzun, Zeynep Vural, Begum Kayar, Seda Tezcan, Mesut Yetkin, Gonul Aslan, Gurol Emekdas, Fatih Koksal Diagnostic Microbiology and Infectious Disease Volume 53, Issue 3, Pages 209-214 (November 2005) DOI: 10.1016/j.diagmicrobio.2005.05.007 Copyright © 2005 Elsevier Inc. Terms and Conditions

Fig. 1 PCR products of cutaneous isolates. Lane 1, 100 bp stepladder; lanes 2–8, L. tropica (403 bp); lanes 9–11, L. infantum (435 bp) on 2.5% agarose gel. Diagnostic Microbiology and Infectious Disease 2005 53, 209-214DOI: (10.1016/j.diagmicrobio.2005.05.007) Copyright © 2005 Elsevier Inc. Terms and Conditions

Fig. 2 RFLP band patterns of cutaneous isolates after digestion with EaeI. Lane 1, 100-bp stepladder; lanes 2–8, L. tropica (227, 190 bp); lanes 9–11, L. infantum (327, 108 bp) on 2% agarose gel. Upper faint bands are the remaining DNA from digestion. Diagnostic Microbiology and Infectious Disease 2005 53, 209-214DOI: (10.1016/j.diagmicrobio.2005.05.007) Copyright © 2005 Elsevier Inc. Terms and Conditions

Fig. 3 PCR products of visceral isolates. Lane 1, 100-bp stepladder; lanes 2–6, L. infantum (435 bp) on 2% agarose gel. Diagnostic Microbiology and Infectious Disease 2005 53, 209-214DOI: (10.1016/j.diagmicrobio.2005.05.007) Copyright © 2005 Elsevier Inc. Terms and Conditions

Fig. 4 RFLP band patterns of visceral isolates after digestion with EaeI. Lane 1, stepladder (100 bp); lanes 2–6, L. infantum (327, 108 bp) on 2.5% agarose gel. Upper faint bands are the remaining DNA from digestion. Diagnostic Microbiology and Infectious Disease 2005 53, 209-214DOI: (10.1016/j.diagmicrobio.2005.05.007) Copyright © 2005 Elsevier Inc. Terms and Conditions

Fig. 5 RFLP band patterns of reference Leishmania isolates. Lane 1, stepladder (100 bp); lanes 2 and 5, L. infantum (NYC/CN/80/RD13); lanes 3 and 4, L. donovani (HOM/IN/01/2001 and HOM/CN/80/SmanA); lanes 6 and 7, L. tropica (IROS/NA/87/8688 and HOM/KE/81/NLB030B) on 2.5% agarose gel. Upper faint bands are the remaining DNA from digestion. Diagnostic Microbiology and Infectious Disease 2005 53, 209-214DOI: (10.1016/j.diagmicrobio.2005.05.007) Copyright © 2005 Elsevier Inc. Terms and Conditions