BIOMARKERS for Oxidative Stress in Veterinary Medicine, Part 2

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BIOMARKERS for Oxidative Stress in Veterinary Medicine, Part 2 W. Jean Dodds, DVM & Denis M. Callewaert, PhD AHVMA 2016 Columbus, OH

Oxford Biomedical Research & Hemopet Study 3 groups of 20 Hemopet greyhound dogs each Normal dry dog food diet Low-fat light kibble diet Home made custom diet for food intolerant dogs Samples of whole blood, serum, and saliva collected at day 0, then at 2 weeks, 1 month and 2 months Measure catalase and/or superoxide dismutase in blood, as markers for transcription factor Nrf2 (nuclear factor 2 p45-related factor) activation rather than glutathione Measure interleukin – 6; tumor necrosis factor –alpha, and 15F2t-isoprostane (oxidation product of arachidonic acid) Measure TBARS : specific for malonaldehyde

Dietary Factors Known to Induce Nrf2 Turmeric, R-α-Lipoic acid, tert-butylhydroquinone, sulforaphane and resveratrol Cause increases in several enzymes (including catalase and superoxide dismutase)