Protein Synthesis Lab

DNA mRNA Protein

STEP 1 First things first…. Take 1 notecard and write all 3 group members names on the card.

Building a DNA Molecule STEP 2 Building a DNA Molecule You have been given the DNA template strand. Remember DNA is read is 3’s – draw a vertical line between every triplet. Fill in the complementary strand of the DNA molecule. Draw a vertical line between every triplet. Each student is in charge of 3 triplets. Write each student’s name under the triplets they are in charge of.

Do the template strand first!

Building the DNA Template Strand STEP 3 Each student needs 3 notecards. 1 notecard = 1 triplet Copy down the triplets of the template strand you are assigned. Using the yarn, begin tying your template strand together.

Now build the complementary strand!

Building the Complementary Strand STEP 4 Building the Complementary Strand Each student needs 3 notecards. 1 notecard = 1 triplet Copy down the triplets of the complementary strand you are assigned. Using the yarn, begin tying your complementary strand together.

Tie the template strand and complementary strands together STEP 5 Tie the template strand and complementary strands together

Lastly STEP 6 Fold up your completed DNA molecule and place in a Ziploc bag. Also place the card with your names on it in the Ziploc bag where I can see it. Turn in to the box at the front. Please clean up your area.

*Warm Up* Find exit ticket. Title page 88: Transcription Practice & Exit Ticket Self-grade and glue in.

https://www.youtube.com/watch?v=bKIpDtJdK8Q

TODAY’S TASK DNA mRNA Protein

Work Time Push desks together. Send 1 person up to get your Ziploc bag, a template page, 9 notecards, and 1 piece of yarn. Go to Moodle. Open the Protein Synthesis Lab PowerPoint. Follow the directions to transcribe the DNA molecule you made.

Transcribe the DNA DNA to mRNA

STEP 1 Label the 3’ and 5’ end of your TEMPLATE STRAND. Label the 5’ and 3’ end of your COMPLIMENTARY STRAND. If your DNA template strand reads 3’ to 5’, then your mRNA strand will read 5’ to 3’. STEP 1

Use the 3’ to 5’ TEMPLATE strand of your DNA molecule to transcribe your mRNA molecule. Assign each person 3 codons to write. STEP 2

Using 9 note cards and string, create your mRNA strand. STEP 3

Have your teacher check your names off that you have successfully completed your DNA strand and your mRNA strand. STEP 4

STEP 5 CREATE Using the models you have made, create a short video, stop animation, gif, etc. that explains how transcription works. Email to: aaron.gunn@nisdtx.org

STEP 6 DONE? Fold up your DNA and mRNA strands carefully and place in your Ziploc bag. Make sure your name card is able to be seen. Place in your class period’s box. Clean up.

Translate the mRNA mRNA to Protein

TODAY’S TASK DNA mRNA Protein

Using the codon chart or wheel, translate your mRNA strand into a sequence of amino acids. STEP 1

Write these amino acids on your notecards and string together to create an amino acid chain. Show your teacher your completed sequence. STEP 2

STEP 3 CREATE Use your models! Create a 30 second – 1 minute video, gif, stop animation, etc. explaining how translation works. Email to: aaron.gunn@nisdtx.org

STEP 4 DONE? Fold up your DNA, mRNA strand, amino acid sequence carefully and place in your Ziploc bag. Make sure your name card is able to be seen. Place in your class period’s box. Clean up.