Material for Introductory Microbiology

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Presentation transcript:

Material for Introductory Microbiology Computer Filename: Protsynb.ppt Protein Synthesis Material for Introductory Microbiology

Overview Genes are composed of DNA within the chromosome Genes code for proteins DNA is transcripted into RNA RNA is translated into protein by ribosomes Genes regulated by control of RNA production

Lecture Outline Protein synthesis tRNA structure adding amino acids to tRNA ribosome structure ribosome attachment protein synthesis :initiation, elongation, attachment

Concepts from OAC Structure & Function of DNA & RNA Genetic code Transcription & translation Protein synthesis

Background Terms & Concepts Structure of DNA & RNA Ribosomes Enzymes

CODONS sequences of three adjacent nucleotides that code for one amino acid or polypetide termination 64 codons encrypt of ~ 20 amino acids degenerate ACU, ACC,ACA, ACG threonine Start codon AUG (also codes for methionine) Stop codon UAA,UAG,UGA (nonsense codons)

Interpreting the Genetic Code Two step process :Transcription -translation Transcription DNA --> RNA Translation RNA--> protein

Transcription Production of an RNA copy of the gene occurs in nucleus DNA unravels- single strand RNA Polymerase binds to Promotor site Reads to stop codon assembles complementary single strand of RNA (primary RNA) Disengages when reaches stop codon

Protein Synthesis Formation of an initiation complex Chain elongation Chain termination

Concept Map: Protein Synthesis

Initiation of Protein Synthesis

Peptide Elongation

tRNA-AA Binding

Protein Membrane Signal Sequences

Cytoplasmic signal sequences

Transmembrane Sequences

EM of ER

Golgi Signalling

Overview of Protein Synthesis

Transcription-translation Overview