Material for Introductory Microbiology Computer Filename: Protsynb.ppt Protein Synthesis Material for Introductory Microbiology
Overview Genes are composed of DNA within the chromosome Genes code for proteins DNA is transcripted into RNA RNA is translated into protein by ribosomes Genes regulated by control of RNA production
Lecture Outline Protein synthesis tRNA structure adding amino acids to tRNA ribosome structure ribosome attachment protein synthesis :initiation, elongation, attachment
Concepts from OAC Structure & Function of DNA & RNA Genetic code Transcription & translation Protein synthesis
Background Terms & Concepts Structure of DNA & RNA Ribosomes Enzymes
CODONS sequences of three adjacent nucleotides that code for one amino acid or polypetide termination 64 codons encrypt of ~ 20 amino acids degenerate ACU, ACC,ACA, ACG threonine Start codon AUG (also codes for methionine) Stop codon UAA,UAG,UGA (nonsense codons)
Interpreting the Genetic Code Two step process :Transcription -translation Transcription DNA --> RNA Translation RNA--> protein
Transcription Production of an RNA copy of the gene occurs in nucleus DNA unravels- single strand RNA Polymerase binds to Promotor site Reads to stop codon assembles complementary single strand of RNA (primary RNA) Disengages when reaches stop codon
Protein Synthesis Formation of an initiation complex Chain elongation Chain termination
Concept Map: Protein Synthesis
Initiation of Protein Synthesis
Peptide Elongation
tRNA-AA Binding
Protein Membrane Signal Sequences
Cytoplasmic signal sequences
Transmembrane Sequences
EM of ER
Golgi Signalling
Overview of Protein Synthesis
Transcription-translation Overview