Are leukocytes in asthmatic patients aging faster

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Are leukocytes in asthmatic patients aging faster Are leukocytes in asthmatic patients aging faster? A study of telomere length and disease severity Shigenori Kyoh, MD, PhD, Narayanan Venkatesan, PhD, Audrey H. Poon, PhD, Michiyoshi Nishioka, MD, PhD, Ting-Yu Lin, MD, Carolyn J. Baglole, PhD, David H. Eidelman, MD, Qutayba Hamid, MD, PhD Journal of Allergy and Clinical Immunology Volume 132, Issue 2, Pages 480-482.e2 (August 2013) DOI: 10.1016/j.jaci.2013.02.010 Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Comparison of absolute telomere length of peripheral blood between patients with severe asthma (n = 8), patients with mild asthma (n = 6), and control subjects (n = 15) (P < .05, Kruskal-Wallis; P < .05, patients with severe asthma vs control subjects). Kruskal-Wallis test and Dunn post hoc test were used for across-group comparison and between-group comparison, respectively. Results are expressed as mean ± SE. Journal of Allergy and Clinical Immunology 2013 132, 480-482.e2DOI: (10.1016/j.jaci.2013.02.010) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Representative photomicrographs showing immunohistochemical staining for hTERT in bronchial biopsies. A, Patient with severe asthma. B, Patient with mild asthma. C, Healthy control. D, Subject with COPD. E, Negative control. F, Tonsil tissue. White arrowheads point to immunopositive cells (brown stain) for hTERT expression. Original magnification ×400. Journal of Allergy and Clinical Immunology 2013 132, 480-482.e2DOI: (10.1016/j.jaci.2013.02.010) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 hTERT protein expression in the submucosa of bronchial biopsies of patients with mild asthma, patients with severe asthma, control subjects, and patients with COPD. The number of hTERT immunopositive cells in bronchial biopsies of control subjects was significantly higher when compared with biopsies from patients with mild or severe asthma or COPD (P = .009, Kruskal-Wallis; P < .001, patients with severe asthma vs control subjects, patients with mild asthma vs control subjects, patients with COPD vs control subjects). Kruskal-Wallis test and Bonferroni correction were used for across-group comparison and between-group comparison, respectively. Results (number of cells per mm2 submucosa) are expressed as mean ± SE. Journal of Allergy and Clinical Immunology 2013 132, 480-482.e2DOI: (10.1016/j.jaci.2013.02.010) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 Representative photomicrographs showing immunohistochemical double staining of bronchial mucosa for the purpose of identifying the cellular phenotype and their expression for hTERT. A, CD4+ T lymphocytes. B, CD68+ monocytes/macrophages. C, Elastase+ neutrophils. D, Vimentin+ fibroblasts. E, EG2 (eosinophil granule)+ eosinophils. F, Alpha-SMA+ smooth muscle cells. CD4-, CD68-, elastase-, vimentin-, EG2-, and α-SMA-positive cells are stained red (with the fast red complex [alkaline phosphatase-antialkaline phosphatase]), hTERT-positive cells are stained brown (avidin-biotin complex technique), and nucleus is stained blue. Cells showing colocalization are stained brown-red-orange (white arrows). Double immunostaining revealed that lymphocytes, monocytes/macrophages, neutrophils, fibroblasts eosinophils, and smooth muscle cells expressed TERT protein. Original magnification ×1000. SMA, Smooth muscle actin. Journal of Allergy and Clinical Immunology 2013 132, 480-482.e2DOI: (10.1016/j.jaci.2013.02.010) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions