OmcB PROTEIN EXPRESSED IN ADENOVIRAL VECTOR INDUCES PROTECTION AGAINST CHLAMYDIA MURIDARUM VAGINAL INFECTION Presenting author: Ekaterina A. Koroleva*,

Slides:



Advertisements
Similar presentations
Protective Immune Response in BALB/c Mice Induced by DNA Vaccine of the ROP8 gene of Toxoplasma gondii Wang chunmiao
Advertisements

Objective: To characterize the relationship between hGBPs and chlamydial growth in cultured cells. Thais Herrero Geraldino.
John R. LaMontagne Memorial Symposium on Pandemic Influenza Research April 4-5, 2005 Institute of Medicine Working Group Five: Immunology, Assay Standardization,
CATEGORY: VACCINES & THERAPEUTICS HIV-1 Vaccines Shokouh Makvandi-Nejad, University of Oxford, UK HIV-1 Vaccines © The copyright for this work resides.
Test of a new liposomal adjuvant for the commercial influenza vaccine in ferret Martel C. a, Hammer Jensen T. a, Viuff B. a, Nielsen L.P. b, Agger E.M.
In DNA vaccine, After plasmid construction and preparation adjuvants ,The mice were divided into 7 groups [pcDNA3/TSA, pcDNA3/TSA +dendrimer and pcDNA3/TSA.
HIV-1 Vaccines Shokouh Makvandi-Nejad, University of Oxford, UK
The association between anti-PcrV titers and protective effects against P. aeruginosa in PcrV vaccinated mice Saeko Hamaoka1, Yoshifumi Naito1, Hideya.
Training Set Clinicopathological parameters of the training set
Development of Med28 Specific Monoclonal Antibodies
Up-Regulation of Activating Transcription Factor-5 Suppresses SAP Expression to Activate T Cells in Hemophagocytic Syndrome Associated with Epstein-Barr.
Figure 1 Protective effects of acellular pertussis vaccines (A–F) in the respiratory infection model. Mice were challenged by exposure to an aerosol of.
From: Development of a Gene Therapy Virus with a Glucocorticoid-Inducible MMP1 for the Treatment of Steroid Glaucoma Invest. Ophthalmol. Vis. Sci ;51(6):
A study of high risk African American women, 15 to 21 years of age
In Vivo Production of Monoclonal Antibodies by Gene Transfer via Electroporation Protects against Lethal Influenza and Ebola Infections  Chasity D. Andrews,
by Peter Ruf, and Horst Lindhofer
An anti-CD20–IL-2 immunocytokine is highly efficacious in a SCID mouse model of established human B lymphoma by Stephen D. Gillies, Yan Lan, Steven Williams,
Volume 26, Issue 4, Pages (April 2018)
Volume 5, Issue 1, Pages (January 2009)
Complexes of trophoblastic peptides and heat shock protein 70 as a novel contraceptive vaccine in a mouse model  Mei Han, Yuan Yao, Wangjiang Zeng, Yanfang.
Virus-specific IgE enhances airway responsiveness on reinfection with respiratory syncytial virus in newborn mice  Azzeddine Dakhama, PhD, Young-Mok Lee,
Immunologic Characterization of HIV-Specific DNA Vaccine
Volume 17, Issue 8, Pages (August 2009)
Anti-ACTL7a antibodies: a cause of infertility
Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic receptor DEC-205 stimulates protective T-cell responses by Cagan Gurer, Till.
Volume 6, Issue 2, Pages (August 2009)
Volume 22, Issue 7, Pages (July 2014)
Durbaka V.R Prasad, Sabrina Richards, Xoi Muoi Mai, Chen Dong  Immunity 
Volume 15, Issue 12, Pages (December 2007)
Forkhead box protein 3 demethylation is associated with tolerance induction in peanut- induced intestinal allergy  Meiqin Wang, MD, PhD, Ivana V. Yang,
Prevention of Genital Herpes Simplex Virus Type 1 and 2 Disease in Mice Immunized with a gD-Expressing Dominant-Negative Recombinant HSV-1  Richard Brans,
Volume 26, Issue 4, Pages (April 2018)
Volume 26, Issue 2, Pages (February 2018)
A predominantly articular cartilage-associated gene, SCRG1, is induced by glucocorticoid and stimulates chondrogenesis in vitro  Kensuke Ochi, M.D., Ph.D.,
Volume 23, Issue 10, Pages (October 2015)
Volume 125, Issue 1, Pages (July 2003)
Phospholipid Scramblase 1 Mediates Type I Interferon-Induced Protection against Staphylococcal α-Toxin  Miroslaw Lizak, Timur O. Yarovinsky  Cell Host.
Volume 18, Issue 11, Pages (November 2010)
Hemagglutinin-targeting Artificial MicroRNAs Expressed by Adenovirus Protect Mice From Different Clades of H5N1 Infection  Xinying Tang, Hongbo Zhang,
Volume 9, Issue 3, Pages (November 2014)
Volume 18, Issue 9, Pages (September 2010)
In Vivo Production of Monoclonal Antibodies by Gene Transfer via Electroporation Protects against Lethal Influenza and Ebola Infections  Chasity D. Andrews,
All-Trans-Retinoic Acid Induces Interleukin-8 via the Nuclear Factor-κB and p38 Mitogen-Activated Protein Kinase Pathways in Normal Human Keratinocytes 
Volume 15, Issue 1, Pages (July 2001)
Diagnostic value of an ELISA using a recombinant 54-kDa species-specific protein from Chlamydia pneumoniae  J-M. Sueur, K. Beaumont, T. Cabioch, J. Orfila,
Volume 5, Issue 1, Pages (January 2009)
Volume 12, Issue 2, Pages (August 2005)
Volume 9, Issue 5, Pages (November 1998)
Thomas S. Griffith, Elizabeth L. Broghammer  Molecular Therapy 
Volume 22, Issue 1, Pages (January 2014)
Volume 6, Issue 4, Pages (April 2016)
Volume 20, Issue 5, Pages (May 2012)
Volume 19, Issue 7, Pages (May 2017)
Molecular Therapy - Oncolytics
Volume 25, Issue 12, Pages e3 (December 2018)
IFN-γ Represses IL-4 Expression via IRF-1 and IRF-2
Volume 17, Issue 10, Pages (October 2009)
John M. Lamar, Vandana Iyer, C. Michael DiPersio 
Volume 7, Issue 5, Pages (May 2010)
Volume 9, Issue 3, Pages (March 2004)
Volume 6, Issue 5, Pages (November 2002)
Volume 3, Issue 5, Pages (May 2001)
Volume 20, Issue 4, Pages (April 2012)
Dissecting the Multifactorial Causes of Immunodominance in Class I–Restricted T Cell Responses to Viruses  Weisan Chen, Luis C. Antón, Jack R. Bennink,
Molecular Therapy - Oncolytics
Synergistic combination of valproic acid and oncolytic parvovirus H‐1PV as a potential therapy against cervical and pancreatic carcinomas VPA treatment.
Volume 16, Issue 12, Pages (December 2008)
Atsushi Yamanaka, Eiji Konishi
Volume 22, Issue 1, Pages (January 2014)
Comparison of C. muridarum organisms with or without a deficiency in pGP3 for their ability to induce hydrosalpinx in the upper genital tract and spread.
Presentation transcript:

OmcB PROTEIN EXPRESSED IN ADENOVIRAL VECTOR INDUCES PROTECTION AGAINST CHLAMYDIA MURIDARUM VAGINAL INFECTION Presenting author: Ekaterina A. Koroleva*, Natalia V. Kobets, Dmitrij N. Shcherbinin, Maksim M. Shmarov, Nelly A. Zigangirova. Gamaleya Research Institute for epidemiology and microbiology, Moscow, Russia Abstract Methods Results Conclusion Genital infection with Chlamydia trachomatis is gradually becoming one of the major threats to the reproductive health worldwide with especially high rates of infection in younger adults. Due to a range of the reasons such as widely asymptomatic nature of infection, lack of timely diagnostics and adverse effects on host immunity currently available antimicrobial treatment is not sufficient to restrict the spread of infection. Vaccine development is a highly desirable for more efficient protection of reproductive health of the world population. In this study we present an approach of vaccination that combines the benefits of mucosal delivery of genetically expressed Chlamydia muridarum protein OmcB. OmcB is highly conserved among Chlamydia species, suggesting that it plays a significant role during intracellular chlamydial infection. OmcB may function as an adhesin for chlamydial invasion into host cells, since heparin can block the infectivity of some C. trachomatis serovars by binding to an N-terminal peptide of OmcB [1]. Plasmid pShuttle-CMV, containing gene omcB (Ad-OmcB) was constructed for this study. Expression of omcB gene was confirmed by western blot. To evaluate the protective potential of the constructed vaccine candidate female BALB/c mice were immunized intranasal with a single dose of either 108 plaque forming units (PFU) of Ad-OmcB, 3 weeks prior to intravaginal infection with C. muridarum. Empty vector immunized and primary infected mice were used as controls. Mice were hormonally synchronized by subcutaneous infection with progesterone (“Covinan”) 5 days prior to a vaginal challenge [2]. 4 weeks later treated mice were infected intravaginally with 106 (inclusion-forming units) IFU/mouse of C. muridarum strain Nigg. Infection was monitored by measuring infection forming units (IFU) from vaginal swabs that were collected from day 3 to 20 post challenge. Sera samples were obtained at 14 day post immunization for assessing specific antibodies by immunofluorescence assay (IFA) and ELISA. We designed adenovirus construction expressing OmcB C. muridarum. Expression was carried out according to the commercial protocol of «AdEasy Adenoviral vector system» kit (Stratagene) [3]. Vaginal swab samples were obtained at days 3, 6, 9, 15 and 20 and the protective effects of Ad- OmcB expressed as median lg IFU/ml. At day 3 post infection we observed significant difference between vaccinated and non-vaccinated mice. In control group 100 % mice infected intravaginally with C. muridarum had high levels of Chlamydia inclusions (0,5х106 IFU) in swabs, peaking at day 6. (Fig.1.) Empty vector immunization gave no protection at all, while in the group of omcB immunized mice the level of infection was decreased starting from day 3 post infection. Higher levels of serum anti-chlamydia antibodies were observed in Ad-OmcB group compared to controls at day 14 post immunization (Fig.2 and 3). OmcB expressed in adenoviral vector delivered in a single dose via mucosal surfaces is capable to induce protection against C. muridarum vaginal infection and increase in specific antibodies in response to immunization. Neutralization assay revealed the presence of neutralizing antibodies in sera of mice immunized with Ad-OmcB. Please export the Keynote document as a PDF (File – Save as – PDF – Image Quality – Best) and upload the PDF into the system. Please use the font in the document or a similar one and do not use a font size smaller than 16. Reference 1.Shuping H.L.L., Zhangsheng Y., Manli Q., Quanzhong L. and Zhong G. Chlamydia trachomatis Outer Membrane Complex Protein B (OmcB) Is Processed by the Protease CPAF. J Bacteriol. Mar 2013; 195(5): 951–957. 2. N. Kobets, E. Koroleva, N. Zigangirova. Impaired early intravaginal cell recruitment in response to Chlamydia muridarum in mice pretreated with progesterone., Zh Mikrobiol Epidemiol Immunobiol. April 2012,Vol. 18, Issue Supplement s3, Pages 841 3. Tutykhina I.L., Logunov D.Y., Shcherbinin D.N., Shmarov M.M., Tukhvatulin A.I., Naroditsky B.S., Gintsburg A.L. Development of adenoviral vector-based mucosal vaccine against influenza. J Mol Med. 2011. №89 (4). PP. 331-341. Ad-null IFU/ml C. muridarum Ad-mOmcB Days post infection Figure 1. Vaginal chlamydial shedding was measured post-challenge. Figure 2. Suppression of chlamydia infection in cell culture by serum neutralizing antibodies. Figure 3. Antibody production triggered by vaccination Ad-mOmcB-Fc.