From: Low Levels of Hydrogen Peroxide Stimulate Corneal Epithelial Cell Adhesion, Migration, and Wound Healing Invest. Ophthalmol. Vis. Sci.. 2011;52(3):1723-1734. doi:10.1167/iovs.10-5866 Figure Legend: Effect of low H2O2 on mouse corneal epithelial wound healing in vivo. (A) C57BL/6 mice were used for this in vivo study. Each cornea was scraped at the center area with a spatula to form an epithelial wound. These animals were divided into four groups, and the eyes were treated every 6 hours up to 48 hours with the following eye drop formulations: The first group was treated with an eye drop formulation containing 400 μM H2O2; the second group was treated with an eye drop containing 400 μM H2O2 + 40 mM NAC; the third group was treated with an eye drop containing 40 mM NAC; and the fourth group (control) was treated with PBS without any additive. Photos were taken at time intervals of 0, 12, 24, 36, and 48 hours after using fluorescein staining of the corneas to allow clear demarcation of the wounding surface (green). White dotted outline indicates the fluorescein-stained wound region. (B) Quantification of the wound healing in the in vivo mouse study. The area of the healed region in each corneal wound was quantified, expressed as percentage rate of wound healing as a function of time (hours) and shown as mean ± SE. The data at 36 hours in H2O2, NAC, and H2O2 + NAC group was each compared with control group (*P < 0.01, n = 4). Date of download: 10/14/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.