Figure 1. ChIL-7 gene amplification and its eukaryotic expression vector construction. (A) Chicken IL-7 cDNA gene amplified by RT-PCR: Lane 1, IL-7 gene.

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Figure 1. ChIL-7 gene amplification and its eukaryotic expression vector construction. (A) Chicken IL-7 cDNA gene amplified by RT-PCR: Lane 1, IL-7 gene PCR product; M, DL2000™ DNA marker. (B) The gene sequence of chicken IL-7 and its encoded amino acids: The italic letters present the signal peptide. (C) Eukaryotic expression vector of chIL-7 gene driven by CMV promoter. (D) Restriction analysis for the chIL-7 expression vector: Lane 1, Xho I and Kpn I double digestion; Lane 2, chIL-7 expression vector; M, DL2000™ DNA marker. From: Molecular cloning of chicken IL-7 and characterization of its antiviral activity against IBDV in vivo Poult Sci. 2016;95(11):2647-2654. doi:10.3382/ps/pew251 Poult Sci | © The Author 2016. Published by Oxford University Press on behalf of Poultry Science Association.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

Figure 2. Detection of the recombinant chicken IL-7 by Western Blot Figure 2. Detection of the recombinant chicken IL-7 by Western Blot. (A) Detection of chIL-7 in the culture medium from pcDNA-chIL7/MH vector-transfected cells (Lane 1) and pcDNA3.1A empty vector-transfected cells (Lane 2); M, prestained protein markers. (B) Purified chIL-7 proteins eluted from Ni-NTA Agarose column: M, prestained protein markers; Lanes 1–4, elutes 1–4 of chIL-7. (C) Detection of chIL-7 from the cell lysate (Lane 1) and the culture medium (Lane 2); M, prestained protein markers. From: Molecular cloning of chicken IL-7 and characterization of its antiviral activity against IBDV in vivo Poult Sci. 2016;95(11):2647-2654. doi:10.3382/ps/pew251 Poult Sci | © The Author 2016. Published by Oxford University Press on behalf of Poultry Science Association.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

Figure 3. The stimulation index of chIL-7 on 2E8 cells at different doses measured by MTT. From: Molecular cloning of chicken IL-7 and characterization of its antiviral activity against IBDV in vivo Poult Sci. 2016;95(11):2647-2654. doi:10.3382/ps/pew251 Poult Sci | © The Author 2016. Published by Oxford University Press on behalf of Poultry Science Association.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

Figure 4. The effects of chIL-7 on IBDV titers in bursal tissues and survival of IBDV-infected chicken. (A) The effects of chIL-7 on IBDV titers in bursal tissues of IBDV-infected chicken. (B) The effects of chIL-7 at different doses (1, 2.5, and 5 mg/kg/d) on the mortality of IBDV-infected chicken. The experimental data are presented as means ± standard deviation. From: Molecular cloning of chicken IL-7 and characterization of its antiviral activity against IBDV in vivo Poult Sci. 2016;95(11):2647-2654. doi:10.3382/ps/pew251 Poult Sci | © The Author 2016. Published by Oxford University Press on behalf of Poultry Science Association.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

Figure 5. The effects of chIL-7 on lymphocyte proliferation and INF-γ production. Effects of chIL-7 on bursal (A) and splenic (B) lymphocyte proliferations isolated from IBDV-uninfected chicken measured by MTT. (C) Effects of chIL-7 on splenic lymphocyte proliferation isolated from IBDV-infected chicken, stimulated with Con A for 48 h, measured by MTT. (D) Effects of chIL-7 on INF-γ productions in the culture medium of the splenic lymphocytes isolated from IBDV-uninfected chicken measured by ELISA. (E) Effects of chIL-7 on INF-γ mRNA expression in splenic tissues of IBDV-infected chicken measured by real-time PCR. The experimental data are presented as means ± standard deviation. * P < 0.05; ** P < 0.01. From: Molecular cloning of chicken IL-7 and characterization of its antiviral activity against IBDV in vivo Poult Sci. 2016;95(11):2647-2654. doi:10.3382/ps/pew251 Poult Sci | © The Author 2016. Published by Oxford University Press on behalf of Poultry Science Association.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

Figure 6. The effects of chIL-7 on chicken TGF-β expression in vitro and in vivo. (A) Effects of chIL-7 on TGF-β expression of splenic lymphocytes isolated from IBDV-uninfected chicken measured by ELISA. (B) Effects of chIL-7 on TGF-β mRNA levels in splenic tissues of IBDV-infected chicken for 8 d measured by real-time PCR. The experimental data are presented as means ± standard deviation. * P < 0.05; ** P < 0.01. From: Molecular cloning of chicken IL-7 and characterization of its antiviral activity against IBDV in vivo Poult Sci. 2016;95(11):2647-2654. doi:10.3382/ps/pew251 Poult Sci | © The Author 2016. Published by Oxford University Press on behalf of Poultry Science Association.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com