243 Unit 3 Labs 1 and 4

Histology Slides for the Cardiovascular System Slides are presented in order of magnification As you view the following slides, make sure you can accomplish these goals: Can you identify the vessels and tissue layers observable on the slides? Can you identify the specific structures or layers indicated by the numbered arrows or brackets? At the end of a sequence, you will find the answers to the above for each vessel and layer

4 4 1 2 3 3 Overview (40X), Masson stain for connective tissue

Overview (100X), Masson stain for connective tissue 4 3 6 9 7 10 5 8 1 2 3

13 10 9 12 11 8 Vein or Artery? 400X

Vein or Artery? 400X 13 6 5 7 5 14

Artery, Vein, Nerve Tissue stained with Masson stain Slides 2 through 5: Artery Vein Nerve fascicles Skeletal Muscle Tissue Lumen of the vein (with RBC’s) Vein Tunica Media- smooth muscle layer Vein Tunica Externa- connective tissue layer Lumen of the Artery (with RBC’s) Artery Tunica Externa- connective tissue layer Artery Tunica Media- smooth muscle layer

Artery, Vein, Nerve Tissue stained with Masson stain Slides 2 through 5: 11. Artery Tunica Interna- endothelial cells (simple squamous) Artery Tunica Interna- Internal elastic lamina Capillary Tunica Interna- endothelial cells Vein Tunica Interna- endothelial cells (simple squamous)

3 4 3 2 2 2 1 Overview (40X), stained for elastic fibers

Vein or Artery? 400X 16 12 10 8 2 15 9

Vein or Artery? 400X 10 8 15 9 12

Vein or Artery? 400X 16 3 7 5 6 1

Vein or Artery? 400X 16 14 6 7 5

Artery, Vein, Nerve Tissue stained for Elastic Fibers Slides 8 through 12: Artery Vein Nerve fascicles Skeletal Muscle Tissue Lumen of the vein Vein Tunica Media- smooth muscle layer Vein Tunica Externa- connective tissue layer Lumen of the Artery (with RBC’s) Artery Tunica Externa- connective tissue layer (demonstrating elastic fibers) 10. Artery Tunica Media- smooth muscle layer

Artery, Vein, Nerve Tissue stained for Elastic Fibers Slides 8 through 12: 11. Artery Tunica Interna- endothelial cells (simple squamous) Artery Tunica Interna- internal elastic lamina Capillary Tunica Interna- endothelial cells Vein Tunica Interna- endothelial cells (dark band is due to the elastic fibers in the basement membrane) Artery Tunica Media- external elastic lamina Adipose cells

Vein or Artery? 400X 16 7 6 5 14 8

Vein or Artery? 100X 2 16 8 9 10 12 3 3

8 11 12 10 9 Vein or Artery? 400X

Artery, Vein, Nerve Tissue, Nonspecific Stain Slides 15 through 16: Artery Vein Nerve fascicles Skeletal Muscle Tissue Lumen of the vein Vein Tunica Media- smooth muscle layer Vein Tunica Externa- connective tissue layer Lumen of the Artery (with RBC’s) Artery Tunica Externa- connective tissue layer (demonstrating elastic fibers) 10. Artery Tunica Media- smooth muscle layer

Artery, Vein, Nerve Tissue, Nonspecific Stain Slides 15 through 16: 11. Artery Tunica Interna- endothelial cells (simple squamous) Artery Tunica Interna- internal elastic lamina Capillary Tunica Interna- endothelial cells Vein Tunica Interna- endothelial cells (simple squamous) Artery Tunica Media- external elastic lamina Adipose cells

Specific Kind of Tissue? 400X 3 1 2 2 2

Cardiac Muscle Tissue Slides 20: Nucleus of a Cardiac Muscle Cell Intercalated Disc Branching Fibers Capillary Tunica Interna- endothelial cells

Identify Blood cells 3 2 1 2

Identify Blood cells 4 3

Identify Blood cells 2 4 3

Identify Blood cells 3 6 3 3

7 3 5 5 Identify Blood cells

Identify Blood cells 7 2 3 3 3

Blood Cells Slides 22-27 Basophil Small Lymphocyte Neutrophil Eosinophil Monoctye Large Lymphocyte Platelets