Environmental patterns for Vibrio parahaemolyticus

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Presentation transcript:

Environmental patterns for Vibrio parahaemolyticus Dr. Rachel T. Noble, Dr. Brett A. Froelich, and Kelsey Jesser, UNC Chapel Hill Institute of Marine Sciences

Southeast Most data limited to NC isolates, water samples, and oyster meats Temperature salinity matrices Culture data Patterns for Vp from bioinformatics analyses of 16SrDNA and hsp60 CA: Collaboration with SCCWRP, J. Craig Venter Institute and Scripps Institute of Oceanography for expanding work in southern California. Showing some brand new data.

Decision Matrices Vibrio parahameolyticus in water (CFU/ml) Three years of data collection in eastern North Carolina wild oyster reefs This and other information from specific locations clearly shows that the population of Vp that resides within oysters can be very different from the Vp that is measured in the water At least in the case of the majority of these locations, Vp confirmation was complete but only a small subset of the population was trh or tdh+ Vibrio parahameolyticus in water (CFU/ml) Vibrio parahaemolyticus in oysters (CFU/g)

Vp in oysters by temp/date Vp in water by temp/date Total Vibrio in water by temp/date Total Vibrio in oysters by temp/date

NC Patterns in VP at 5 sites compiled

Wild vs. Farmed Oysters

San Diego Vibrio sampling Monthly Sampling: (Dec 2015-Nov 2016) Los Peñasquitos Lagoon San Diego River (2 sites) Tijuana River Estuary (2 sites) Data available: Culture EnvironmentalChl. A (GF/F filters, 3 reps) Digital PCR Metagenomic 16S and hsp60 Recent Vibrio cases prompted interest in Vp and Vv research-2 deaths Processing: Thanks to Rachel Diner and Andy Allen at JCVI/SIO

Salinity Temperature Salinity (PSU) Temperature (°C) Low salinities at LPL site are around the time of lagoon mouth closure Low rainfall  higher summer salinities Extremely warm temperatures (El Niño year) In the Vibrio proliferation range (>15ºC) at nearly all sites/times

ddPCR Results: tox R (V. parahaemolyticus) From whole community DNA extractions ddPCR on BioRad QX200 using positive control gene blocks and NTCs Some very high copy number samples: Tijuana River Estuary

ddPCR Results: tox R (V. parahaemolyticus) Some very high copy number samples @ Tijuana River Estuary sites (very high salinity: 30-40psu) Correlation with temperature across sites? LPL 22000 > 21000 TJ1 SDR1 toxR Copies/ 100 mL toxR Copies/ 100 mL 32000 34000 > TJ2 SDR2

Discussion…