Edmunds GigaScience 2013 POSTER Open Access

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Edmunds GigaScience 2013 POSTER Open Access GigaGalaxy: A GigaSolution for reproducible and sustainable genomic data publication and analysis Scott Edmunds1,2, Peter Li1,2, Huayan Gao3,4, Ruibang Luo2,5, Dennis Chan1, Alex Wong1, Zhang Yong2, Tin-Lap Lee3,4 Abstract Today's next generation sequencing (NGS) experiments generate substantially more data and are more broadly applicable to previous high-throughput genomic assays. Despite the plummeting costs of sequencing, downstream data processing and analysis create financial and bioinformatics challenges for many biomedical scientists. It is therefore important to make NGS data interpretation as accessible as data generation. GigaGalaxy (http://galaxy.cbiit.cuhk.edu.hk) represents a NGS data interpretation solution towards the big sequencing data challenge. We have ported the popular Short Oligonucleotide Analysis Package (http://soap.genomics.org.cn) as well as supporting tools such as Contiguator2 (http://contiguator.sourceforge.net) into the Galaxy framework, to provide seamless NGS mapping, de novo assembly, NGS data format conversion and sequence alignment visualization. Our vision is to create an open publication, review and analysis environment by integrating GigaGalaxy into the publication platform at GigaScience and its GigaDB database that links to more than 20 TBs of genomic data. We have begun this effort by re-implementing the data procedures described by Luo et al., (GigaScience 1: 18, 2012) as Galaxy workflows so that they can be shared in a manner which can be visualized and executed in GigaGalaxy. We hope to revolutionize the publication model with the aim of executable publications, where data analyses can be reproduced and reused. Keywords: Galaxy, workflows, reproducible research, genome assembly, next generation sequencing, GigaScience Background Example: SOAPdenovo2 Growing replication gap: Linking papers to data and analyses 10/18 microarray papers cannot be reproduced Ioannidis: “Most Published Research Findings Are False” >15X increase in retracted papers in last decade Lack of incentives to make data/methods available Open-Paper Open-Data DOI:10.5524/100038 Data sets 78GB CC0 data Linked to DOI Open-Pipelines DOI Open-Workflows Linked to Analyses DOI:10.5524/100044 GigaSolution: deconstructing the paper Combine and integrate (via citable DOIs): Implement paper pipelines in GigaGalaxy Open-access journal www.gigasciencejournal.com Data Publishing Platform gigadb.org Data Analysis Platform galaxy.cbiit.cuhk.edu.hk GigaGalaxy: screenshot Visualization of results: e.g. GAGE metrics and CONTIGuator 2 outputs: NC_010079.pdf gi_161510924_ref_NC_010063.1_.pdf References Ioannidis et al., Repeatability of published microarray gene expression analyses. Nature Genetics 2009 41: 14 Science publishing: The trouble with retractions Nature 2011 478, 26-28 Ioannidis J. Why Most Published Research Findings Are False. PLoS Med 2005 2(8): e124. Luo R et al., SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler GigaScience 2012, 1:18 Wang, J; et al., (2012): Updated genome assembly of YH: the first diploid genome sequence of a Han Chinese individual (version 2, 07/2012). GigaScience Database. http://dx.doi.org/10.5524/100038. Luo, R; et al., (2012): Software and supporting material for “SOAPdenovo2: An empirically improved memory-efficient short read de novo assembly”. GigaScience Database. http://dx.doi.org/10.5524/100044 Galardini et al.:CONTIGuator: a bacterial genomes finishing tool for structural insights on draft genomes. Source Code for Biology and Medicine 2011 6:11. Acknowledgements Thanks to: Laurie Goodman, Chris Hunter, Xiao Si Zhe, Tam Sneddon (GigaScience), Shaoguang Liang (BGI-SZ), Qiong Luo, Senghong Wang, Yan Zhou (HKUST), Rob Davidson and Mark Viant (Birmingham Uni), Marco Galardini (Unifi) doi:10.6084/m9.figshare.713512 Cite this poster as: GigaGalaxy: A GigaSolution for reproducible and sustainable genomic data publication and analysis. Scott C. Edmunds, Peter Li, Huayan Gao, Ruibang Luo, Dennis Chan, Alex Wong, Zhang Yong, Tin-Lap Lee. figshare http://dx.doi.org/10.6084/m9.figshare.713512 Financial support from: Submit your next manuscript containing large-scale data and workflows to GigaScience and take full advantage of: Correspondence: scott@gigasciencejournal.com 1. BGI HK Research Institute, 16 Dai Fu Street, Tai Po Industrial Estate, Hong Kong SAR, China. 2. BGI-Shenzhen, Beishan Industrial Zone, Yantian District, Shenzhen, China. 3. School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China. 4. CUHK-BGI Innovation Institute of Trans-omics, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China. 5. HKU-BGI Bioinformatics Algorithms and Core Tecnology Research Laboratory & Department of Computer Science, University of Hong Kong, Pok Fu Lam, Hong Kong No space constraints, and unlimited data and workflow hosting in GigaDB and GigaGalaxy Article processing charges for all submissions in 2013 covered by BGI Open access, open data and highly visible work freely available for distribution Inclusion in PubMed and Google Scholar © 2013 Edmunds et al. This is an Open Access poster distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.