Expression of LKB1 tumor suppressor in non–small cell lung cancer determines sensitivity to 2-deoxyglucose Landon J. Inge, PhD, Keith D. Coon, PhD, Michael.

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Expression of LKB1 tumor suppressor in non–small cell lung cancer determines sensitivity to 2-deoxyglucose Landon J. Inge, PhD, Keith D. Coon, PhD, Michael A. Smith, MD, Ross M. Bremner, MD, PhD The Journal of Thoracic and Cardiovascular Surgery Volume 137, Issue 3, Pages 580-586 (March 2009) DOI: 10.1016/j.jtcvs.2008.11.029 Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 LKB1-AMPK signaling and regulation of mammalian target of rapamycin (mTOR). TSC, tuberous sclerosis complex; AMPK, adenosine monophosphate kinase. The Journal of Thoracic and Cardiovascular Surgery 2009 137, 580-586DOI: (10.1016/j.jtcvs.2008.11.029) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Effect of 2-DG in LKB1-null and LKB1-expressing NSCLC cells. A, Immunoblot of LKB1 in H23, H2122, H2009, and H441 NSCLC cell lines. GAPDH was used as a loading control. B, Induction of AMPK phosphorylation by 2-DG. LKB1-positive (H2009, H441) and LKB1-negative (H23, H2122) cell lines were treated with 20 mmol/L of 2-DG for 1 hour. Protein lysates were immunoblotted with an antibody specific to phosphorylated Thr172 AMPK. Note 2-DG induces phosphorylation at Thr172 only in LKB1-expressing cells. Both AMPK and GAPDH were used as loading controls. Blot is representative of two independent experiments. C, LKB1 null NSCLC cell are more sensitive to 2-DG. NSCLC cell lines were treated for 48 hours with indicated concentrations of 2-DG. Bars represent standard error. GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; 2-DG, 2-deoxyglucose; AMPK, adenosine monophosphate activated protein kinase; P-AMPK, adenosine monophospahte kinase phosphorylation. The Journal of Thoracic and Cardiovascular Surgery 2009 137, 580-586DOI: (10.1016/j.jtcvs.2008.11.029) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 Microarray analysis of LKB1-positive (LKB1+) or LKB1-negative (LKB1−) NSCLC cell lines (H2009 and H23, respectively) treated or untreated, in a dose-dependent manner, with 2-DG. Unsupervised hierarchical clustering was performed on 117 apoptosis-related transcripts (left). Excerpted gene clusters are shown at the right. The pattern of gene expression reflects an activation of apoptosis in LKB1-negative cells. Italicized, bolded genes are discussed in the text. 2-DG, 2-Deoxyglucose. The Journal of Thoracic and Cardiovascular Surgery 2009 137, 580-586DOI: (10.1016/j.jtcvs.2008.11.029) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 4 2-DG induces apoptosis in LKB1-null NSCLC cells. A, Activity of caspases 3 and 7 in NSCLC cells after 24 hours of 2-DG treatment at the indicated concentrations. Bars represent standard error and experiment was repeated twice. B, Immunoblot of cleaved PARP. H23 and H2009 cells were treated for 24 hours with 20 mmol/L of 2-DG. Note presence of cleaved PARP in H23, but not H2009 cells. GAPDH was used as a loading control. C, Immunoblot of LKB1 and KDLKB1 in H23 cells after retroviral infection and puromycin selection. GAPDH was used as a loading control. D, 2-DG activation of LKB1 in H23-LKB1, but not H23-KDLKB1 cells. Blot was stripped and probed for AMPK as a loading control. Blot represents two independent experiments. E, LKB1, but not KDLKB1, prevents activation of capase 3 and 7 in H23 cells. H23–LKB1 and H23–KDLKB1 cells were treated for 24 hours with 20 mmol/L 2-DG before analysis as described in Materials and Methods. Bars represent standard error and experiment was repeated twice. F, Immunoblot of cleaved PARP in H23–LKB1 and H23–KDLKB1 after treatment with 20 mmol/L of 2-DG for 24 hours. Blot is representative of two independent experiments. GAPDH was used as a loading control. 2-DG, 2-Deoxyglucose; PARP, poly (ADP-ribose) polymerase; AMPK, adenosine monophosphate activated protein kinase; KD-LKB1, kinase-dead LKB1. The Journal of Thoracic and Cardiovascular Surgery 2009 137, 580-586DOI: (10.1016/j.jtcvs.2008.11.029) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions