Real time PCR data MMP MCF-7 RNA MCF-7 +

Slides:



Advertisements
Similar presentations
Anti-UCH L1 anti-α-tubulin H157 H358 SW403 COLO205 KM1214 CaCO2 RIE-1 SMC HUVEC MCF7 Primary cells Lung cancer cells Small intestine epithelial cells Colon.
Advertisements

What are the three steps in PCR?. Denaturation Hybridization of Primer DNA replication.
Chemical Metrology and Biometry Department National Institute of Metrology (Thailand) CCQM P103.1 “Measurement of multiple RNA transcripts” Duangkamol.
Franck CHATELAIN Q-PCR on DICER project and PKD project Animal facility Myography on PKD project Ingénieur d’études.
Supplemental Figure S1: Diagram to show previously reported interactions between EGFR and 23 other Egr1 target genes using Pathway Studio, Ariadne Inc.
Supplementary Figure Legends: Table 1. List of primers used for Real Time – PCR Supp Fig 1. S100A7-overexpressing MCF7 cells show decreased formation of.
P < n.s. WT 2d BKO 2d C WT: 13 images, KO: 14 images (1000x)
Fig. S1 Beclin1, ATG3 and LC3B mRNA -real-time quantitative PCR HCT-116 HT-29.
Detecting DNA with DNA probes arrays. DNA sequences can be detected by DNA probes and arrays (= collection of microscopic DNA spots attached to a solid.
Short or Tall?. Short Tall Short Tall Short Tall. 3
SP receptor neurokinin 1 receptor (NK1R) is expressed in adipocytes and preadipocytes, and SP interferes with differentiation. 3T3-L1 preadipocytes were.
Supplementary Figure S4
Butler et al Supplementary Figure 1 A B C D - E BL21
Fig. 1 UGT2B15 mRNA levels are stimulated by E2 in a time- and dose-dependent manner in MCF-7 breast cancer cells. A, Time course for E2 treatment. Cells.
اجابة السؤال الاول.
Fig. 2. Effects of UVB on COX-2 and cytokine mRNA expression
Gel electrophoresis analysis Automated DNA analyzer.
Supplemental Digital Content 4
In vitro effect of caffeic acid phenethyl ester on matrix metalloproteinases (MMP-1 and MMP-9) and their inhibitor (TIMP-1) in lipopolysaccharide-activated.
From: Genetic Polymorphisms in the Promoter of the Interferon Gamma Receptor 1 Gene Are Associated with Atopic Cataracts Invest. Ophthalmol. Vis. Sci..
TIMPs MMP MCF-7 RNA MCF-7 + Tenascin 9-16 Long
The Long Non-Coding RNA XIST Interacted with MiR-124 to Modulate Bladder Cancer Growth, Invasion and Migration by Targeting Androgen Receptor (AR) Cell.
From: Adenovirus Type 37 Keratitis in the C57BL/6J Mouse
A B Supplementary figure S3 ShCtl ShLCoR LCoR mRNA (%) Optical density
پروتكل آموزش سلامت به مددجو
محاضرة عامة التقنيات الحيوية (هندسة الجينات .. مبادئ وتطبيقات)
Effective reduction of the interleukin-1β transcript in osteoarthritis-prone guinea pig chondrocytes via short hairpin RNA mediated RNA interference influences.
P38γ mitogen-activated protein kinase suppresses chondrocyte production of MMP-13 in response to catabolic stimulation  D.L. Long, R.F. Loeser  Osteoarthritis.
(x 10-3) (x 10-4) GST P WT mock WT #15 WT mock WT #15
Masanobu Ishii et al. BTS 2017;2:
Interleukin 6 mediates selected effects of Notch in chondrocytes
by Sanjai Sharma, and Alan Lichtenstein
L. Raymond, S. Eck, E. Hays, I. Tomek, M. D. , S. Kantor, M. D. , M
IRF4 regulates cytokine receptor expression in CD8+ T cells.
Development of comprehensive functional genomic screens to identify novel mediators of osteoarthritis  S. Daouti, M.S., B. Latario, M.S., S. Nagulapalli,
Volume 53, Issue 3, Pages (September 2010)
Volume 24, Issue 5, Pages (May 2016)
Volume 129, Issue 1, Pages (July 2005)
Everolimus Silences E2F Target Genes Through TERT-Dependent Epigenetic Histone Modification (A) Western blotting for MCM7 in WT and TERTtg mSMC treated.
Correlation of mouse gene expression with bacterial gene expression.
Molecular Therapy - Nucleic Acids
Masanobu Ishii et al. BTS 2017;2:
Fold Change of hsa-miR-3687 (T/N)(log2)
Ifnlr1 expression on intestinal epithelial cells is necessary for induction of interferon-stimulated genes. Ifnlr1 expression on intestinal epithelial.
Validation of RNA transcription profile differential expression using real-time quantitative PCR. Relative gene expression in cases compared with controls.
Inhibition of β-catenin by the Smad4/BMP pathway.
Thanks to Dr. Pierre Laneuville and
USP2a-dependent miRs deregulation modulates MYC expression.
Negative Regulation of Tumor Suppressor p53 by MicroRNA miR-504
Practical Contents DNA Extraction Gel Electrophoresis
Transcriptional profiling of selected genes by RT-PCR.
Fig. 4 Gene disruption via chip.
Knockdown of endogenous WT-hTER by a short hairpin RNA (siRNA) directed specifically against the human WT telomerase RNA template. Knockdown of endogenous.
Fig. 3 E2F1 interacts with components of the splicing machinery.
CREB1 promotes the induction of endogenous ERα target genes.
A: Gene expression of β-myosin heavy chain (hypertrophic marker) was analyzed on real-time PCR and normalized against 18S expression. n = 8–11 for each.
Validation of the microarray results.
A. baumannii stimulates the autophagy.
Bcl-XL upregulates the expression of genes characteristic to midbrain and hindbrain fates during in vitro differentiation of ES cells. Bcl-XL upregulates.
Figure 4. MicroRNA (miR)-195 and miR-497 directly targets CD274
Experiments were done in triplicates and 5
PELP1 regulates the expression and activities of MMPs in ER-negative cells. PELP1 regulates the expression and activities of MMPs in ER-negative cells.
* Supplementary Fig. 1 (Yamanaka et al.) * * * ** ** ** * * **
RT-PCR analysis of Wnt genes in PLC-PRF-5 and HLF cell lines.
Expression of S100A11 in bulk pancreatic tissues (pancreatic cancer, n = 22; IPMN, n = 18; nonneoplastic pancreas, n = 22). Expression of S100A11 in bulk.
Transcriptional activity and growth of mutant ER in a breast cancer cell line. Transcriptional activity and growth of mutant ER in a breast cancer cell.
Fig. 3. PTEN a target of miR-182-5p
Fig. 4 Dcr-2 binds to the 3′UTR of Toll mRNA.
KIR2DL4 on human mast cells upregulates the invasive activity of HLA-G+ cancer cell lines. KIR2DL4 on human mast cells upregulates the invasive activity.
Overexpression of sRNA-29 changes the expression of 53 genes across the P. denitrificans genome. Overexpression of sRNA-29 changes the expression of 53.
Presentation transcript:

Real time PCR data MMP 3 0.5 1 1.5 2 2.5 3 3.5 MCF-7 RNA MCF-7 + 0.5 1 1.5 2 2.5 3 3.5 MCF-7 RNA MCF-7 + Tenascin 9-16 Long Short 9-14-16 Empty Vector gene/18s MMP 8 0.5 1 1.5 2 2.5 MCF-7 RNA MCF-7 + Tenascin 9-16 Long Short 9-14-16 Empty Vector gene/18s MMP 9 0.5 1 1.5 2 2.5 3 MCF-7 RNA MCF-7 + Tenascin 9-16 Long Short 9-14-16 Empty Vector gene/18s MMP 10 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 MCF-7 RNA MCF-7 + Tenascin 9-16 Long Short 9-14-16 Empty Vector genes/18s MMP 11 0.2 0.4 0.6 0.8 1 1.2 MCF-7 RNA MCF-7 + Tenascin 9-16 Long Short 9-14-16 Empty Vector gene/18s MMP 13 0.5 1 1.5 2 2.5 3 3.5 4 MCF-7 RNA MCF-7 + Tenascin 9-16 Long Short 9-14-16 Empty Vector gene/18s MMP14 0.02 0.04 0.06 0.08 0.1 0.12 0.14 0.16 0.18 0.2 MCF-7 RNA MCF-7 + Tenascin 9-16 Long Short 9-14-16 Empty Vector gene/18s