Grass Seed Mixes Sharon Davidson, RST 2016

Sample # AST # Kind Variety Weight Avg weight of 100 seeds Calculated Purity weight Calculated Bulk weight Measured weight A 282379 Perennial Ryegrass Pinnacle II 50 grams 0.163g 4.063g 40 grams 40.04 grams B 282381 Annual Ryegrass Abundant 0.460g 11.491g 115 grams 115.08 grams C 282380 Tall Fescue Mojave II 0.267g 6.681g 70 grams 70.11 grams

Gamet Centrifugal Divider   Weight Blends (50/50/50) Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 Sample 9 Sample 10 Average AST # 282423 282424 282425 282426 282427 282428 282429 282430 282431 282432 Tall fescue 33.60% 32.39% 33.83% 33.05% 32.81% 32.71% 32.60% 34.16% 35.24% 33.32% Perennial Ryegrass (VFL 0.88%) 48.04% 49.22% 54.43% 51.15% 54.31% 49.03% 54.57% 46.94% 53.07% 48.63% 50.94% Annual Ryegrass (Tetraploid) 17.95% 18.06% 11.49% 15.53% 12.40% 17.88% 12.37% 19.96% 12.27% 15.70% 15.36% Total Pure seed % 99.59% 99.67% 99.75% 99.73% 99.52% 99.62% 99.54% 99.71% 99.50% 99.57% Inert 0.35% 0.33% 0.19% 0.27% 0.46% 0.34% 0.43% 0.36% Other seed 0.06% 0.00% 0.02% 0.04% # Fluorescing seedlings 103 97 63 87 67 98 109 70 89 85 Test Fluorescence 28.45% 29.39% 19.18% 25.30% 21.07% 28.15% 20.19% 31.48% 21.88% 26.83% 25.19%

Weighted Gamet Tall Fescue Tolerance Tall Fescue on Label 33.00%   Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 Sample 9 Sample 10 Average 33.30% 32.70% 33.42% 33.03% 32.91% 32.86% 32.80% 33.58% 34.12% Difference -0.60% 0.61% -0.83% -0.05% 0.19% 0.29% 0.40% -1.16% -2.24% Tolerance from Table 14B OVER LABEL 3.30 Within Tolerance Yes/No Yes Perennial Ryegrass Tolerance Perennial Ryegrass on Label 40.52% 41.11% 43.72% 42.08% 43.66% 41.02% 43.79% 39.97% 43.04% 40.82% -15.04% -16.22% -21.43% -18.15% -21.31% -16.03% -21.57% -13.94% -20.07% -15.63% Annual Ryegrass Tolerance Annual Ryegrass on Label 25.48% 25.53% 22.25% 24.27% 22.70% 25.44% 22.69% 26.48% 22.64% 24.35% 15.05% 14.94% 21.51% 17.47% 20.60% 15.12% 20.63% 13.04% 20.73% 17.30% 3.05 2.97 3.13 No

Gamet Centrifugal Divider Seed Count Blends Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 Sample 9 Sample 10 Average AST # 282443 282444 282445 282446 282447 282448 282449 282450 282451 282452   Tall fescue 28.27% 32.40% 32.54% 32.51% 33.15% 31.22% 32.86% 31.98% 29.62% 32.81% 31.74% Perennial Ryegrass (VFL 0.88%) 37.01% 38.21% 37.65% 30.77% 32.22% 32.15% 32.47% 33.70% 36.34% 34.12% 34.46% Annual Ryegrass (Tetraploid) 34.45% 28.40% 29.45% 36.39% 34.09% 36.24% 34.05% 33.77% 32.80% 33.41% Total pure seed % 99.73% 99.01% 99.64% 99.67% 99.46% 99.61% 99.79% Inert 0.27% 0.93% 0.36% 0.31% 0.52% 0.39% 0.21% 0.00% 0.35% Other seed 0.06% 0.02% 0.04% # Fluorescing seedlings 181 154 158 202 178 196 189 184 182 180 Test Fluorescence 51.42% 45.20% 46.33% 58.48% 51.45% 58.69% 53.39% 53.18% 50.71% 51.85% 52.07%

Gamet Seed Count Blends - Tall Fescue Tolerance Tall Fescue on Label 33.00%   Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 Sample 9 Sample 10 Average 30.64% 32.70% 32.77% 32.76% 33.08% 32.11% 32.93% 32.49% 31.31% 32.91% Difference 4.73% 0.60% 0.46% 0.49% -0.15% 1.78% 0.14% 1.02% 3.38% 0.19% Tolerance from Table 14B 3.30 OVER LABEL Within Tolerance Yes/No No Yes Perennial Ryegrass Tolerance Perennial Ryegrass on Label 35.01% 35.61% 35.33% 31.89% 32.61% 32.58% 32.74% 33.35% 34.67% 33.56% -4.01% -5.21% -4.65% 2.23% 0.78% 0.85% 0.53% -0.70% -3.34% -1.12% Annual Ryegrass Tolerance Annual Ryegrass on Label 33.73% 30.70% 31.23% 34.70% 33.55% 34.62% 33.53% 33.39% 32.90% -1.45% 4.60% 3.55% -3.39% -1.09% -3.24% -1.46% -1.05% -0.77% 0.20%

Dividers An Issue??

Riffle Divider   Weight Blends (50/50/50) Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 Sample 9 Sample 10 Average AST # 282433 282434 282435 282436 282437 282438 282439 282440 282441 282442 Tall fescue 35.36% 31.33% 34.02% 32.31% 31.51% 33.69% 32.86% 32.44% 34.41% 34.06% 33.20% Perennial Ryegrass (VFL 0.88%) 50.53% 52.87% 52.26% 52.68% 56.89% 53.83% 52.96% 55.28% 50.89% 48.93% 52.71% Annual Ryegrass (Tetraploid) 13.86% 15.30% 13.45% 14.51% 11.31% 12.06% 13.75% 11.90% 14.36% 16.82% 13.73% Total Pure seed % 99.75% 99.50% 99.73% 99.71% 99.58% 99.57% 99.62% 99.66% 99.81% 99.64% Inert 0.25% 0.45% 0.27% 0.44% 0.23% 0.42% 0.37% 0.34% 0.32% 0.19% 0.33% Other seed 0.00% 0.05% 0.06% 0.04% 0.02% 0.03% # Fluorescing seedlings 78 83 76 80 62 70 66 84 95 77 Test Fluorescence 23.28% 24.13% 22.26% 21.09% 18.62% 20.71% 22.09% 19.53% 22.70% 27.84% 22.23%

Weighted Riffle Tall Fescue Tolerance Tall Fescue on Label 33.00%   Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 Sample 9 Sample 10 Average 34.18% 32.17% 33.51% 32.66% 32.26% 33.35% 32.93% 32.72% 33.71% 33.53% Difference -2.36% 1.67% -1.02% 0.69% 1.49% -0.69% 0.14% 0.56% -1.41% -1.06% Tolerance from Table 14B OVER LABEL 3.30 Within Tolerance Yes/No Yes Perennial Ryegrass Tolerance Perennial Ryegrass on Label 41.77% 42.94% 42.63% 42.84% 44.95% 43.42% 42.98% 44.14% 41.95% 40.97% -17.53% -19.87% -19.26% -19.68% -23.89% -20.83% -19.96% -22.28% -17.89% -15.93% Annual Ryegrass Tolerance Annual Ryegrass on Label 23.43% 24.15% 23.23% 23.76% 22.16% 22.53% 23.38% 22.45% 23.68% 24.91% 19.14% 17.70% 19.55% 18.49% 21.69% 20.94% 19.25% 21.10% 18.64% 16.18% 2.97 3.05 No

Riffle Divider Seed Count Blends Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 Sample 9 Sample 10 Average AST # 282453 282454 282455 282456 282457 282458 282459 282460 282461 282462   Tall fescue 30.03% 31.36% 31.94% 32.66% 31.51% 29.54% 30.72% 30.90% 29.67% 30.04% 30.84% Perennial Ryegrass (VFL 0.88%) 37.72% 38.48% 39.07% 37.53% 37.83% 38.39% 37.60% 37.63% 38.81% 37.42% 38.05% Annual Ryegrass (Tetraploid) 31.77% 29.84% 28.56% 29.50% 30.41% 31.72% 31.26% 31.27% 31.33% 32.16% 30.78% Total pure seed % 99.52% 99.68% 99.57% 99.69% 99.75% 99.65% 99.58% 99.80% 99.81% 99.62% 99.67% Inert 0.29% 0.32% 0.43% 0.25% 0.23% 0.35% 0.38% 0.19% 0.36% 0.30% Other seed 0.00% 0.06% 0.02% 0.04% 0.01% 0.03% # Fluorescing seedlings 170 159 156 162 164 171 167 166 163 165 Test Fluorescence 45.21% 45.95% 44.00% 45.45% 44.94% 47.06% 46.05% 48.40% 45.61% 47.70% 46.04%

Riffle Calculated Tall Fescue Tolerance Tall Fescue on Label 33.00%   Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 Sample 9 Sample 10 Average 31.52% 32.18% 32.47% 32.83% 32.26% 31.27% 31.86% 31.95% 31.34% Difference 2.97% 1.64% 1.06% 0.34% 1.49% 3.46% 2.28% 2.10% 3.33% 2.96% Tolerance from Table 14B 3.30 Within Tolerance Yes/No Yes No Perennial Ryegrass Tolerance Perennial Ryegrass on Label 35.36% 35.74% 36.04% 35.27% 35.42% 35.70% 35.30% 35.32% 35.91% 35.21% -4.72% -5.48% -6.07% -4.53% -4.83% -5.39% -4.60% -4.63% -5.81% -4.42% OVER LABEL Annual Ryegrass Tolerance Annual Ryegrass on Label 32.39% 31.42% 30.78% 31.25% 31.71% 32.36% 32.13% 32.14% 32.17% 32.58% 1.23% 3.16% 4.44% 3.50% 2.59% 1.28% 1.74% 1.73% 1.67% 0.84%

Number of Fluorescent Seedlings in 400 planted 1 2 3 4 5 6 7 8 9 10 50/50/50 Gamet 103 97 63 87 67 98 109 70 89 85 Riffle 78 83 76 80 62 66 84 95 77 seed count 181 154 158 202 178 196 189 184 182 180 Seed count riffle 170 159 156 162 164 171 167 166 163 165

Tolerances Tables Maybe investigate wider tolerances on mixes? FSA and AOSA rules use similar tolerance tables, if change AOSA would have to open up FSA The one-way test is used in Table 14B to detect seed lots that are poorer quality than that stated in the first analysis (or seed label), however there is no objection to seed lots found to be of better quality than stated in the first analysis (or seed label). Therefore, the results of the first analysis (or seed label) and the second analysis (test) are considered different (i.e., out of tolerance) when the pure seed percentage in the second analysis (test) is significantly lower than the first test (or seed label); or when the inert matter, other crop seed or weed seed percentages are significantly higher in the second test than that in the first test.

Questions? Thoughts?