Method development for the Analysis of Body Odor Using Gas Chromatography-Mass Spectrometer Revathi a/p Rajan Postgraduate Student, School of Health Sciences Masters in Forensic Chemistry Universiti Sains Malaysia Main Supervisor : Dr Nik Fakhuruddin Nik Hassan

Body Odor Combination of volatile and semi-volatile organic compounds produced by human skin Origin of human odor is yet to be discovered. Three categories : Primary, Secondary and Tertiary

Previously employed methods ATD GCMS SPME GCMS Solvent Extraction with GCMS Purge and Trap GCMS

Methods Compared in This Research Headspace Injections Liquid Injections Sample Preparation Optimization Steam Distillation and LLE (non polar solvent) Direct Extraction (non polar solvent) Direct Extraction (polar non polar mixture solvent) Steam Distillation (polar and non polar mixture solvent) Direct Extraction (polar solvent) Qualitative Method Validation Real Sweat Sample Analysis PCA Analysis Analytical parameters optimization Sample collection optimization Sample preparation optimization Collection Material Optimization Real Sweat Sample Analysis

Static Headspace Injection Inlet Temperature : 200ºC Maximum Oven Temp : 200ºC Transfer Line Temp :250ºC Sample Collection : 60 min Inlet Temperature : 200ºC Maximum Oven Temp : 200ºC Transfer Line Temp :230ºC Sample Collection : 20 min Inlet Temperature : 200ºC Maximum Oven Temp : 200ºC Transfer Line Temp :230ºC Sample Collection : 20 min Inlet Temperature : 200ºC Maximum Oven Temp : 270ºC Transfer Line Temp :250ºC Sample Collection : 45 min Inlet Temperature : 200ºC Maximum Oven Temp : 200ºC Transfer Line Temp :250ºC Sample Collection : 20 min Inlet Temperature : 250ºC Maximum Oven Temp : 280ºC Transfer Line Temp :280ºC Sample Collection : 45 min

Polar and Non Polar Mixture Liquid Injection Direct Extraction Polar and Non Polar Mixture Direct Extraction Polar Solvent

Subject Analysis Results Inter Individual Variation Intra Individual Variation

Conclusion Direct extraction using methanol as solvent was the optimum method for the analysis of the less volatile component present in the body odor Study of the odor component revealed that individualization can be achieved using primary odor component and that odor is unique for every individual.

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