Development and clinical evaluation of a novel fully automated qualitative PCR assay for the diagnosis of anogenital herpes simplex virus infection Steve.

Slides:

Advertisements

Similar presentations

MALDI-TOF is not useful in the diagnosis of catheter colonization based on superficial cultures: results from an in vitro study María Guembe, María Jesús.

Advertisements

Models for the organisation of hospital infection control and prevention programmes B. Gordts Clinical Microbiology and Infection Volume 11, Pages

Performance of cobas® 4800 and m2000 real-time™ assays for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in rectal and self-collected vaginal.

Detection of parvovirus B19 in synovial fluids of patients with osteoarthritis Bahadir Aslan, Mehmet Sami Serin, Gonul Aslan, Aydiner Kalaci, Ahmet Nedim.

Comparison of the Roche RealTime ready Influenza A/H1N1 Detection Set with CDC A/H1N1pdm09 RT-PCR on samples from three hospitals in Ho Chi Minh City,

P.-Y. Lévy Clinical Microbiology and Infection

R. Dumke, H. von Baum, P.C. Lück, E. Jacobs

A. Bergman, D. Heimer, N. Kondori, H. Enroth

Comparison of a multiplexed bead-based assay with an immunofluorescence and an enzyme-immuno assay for the assessment of Epstein–Barr virus serologic.

Detection of Panton–Valentine leukocidin gene in Staphylococcus aureus by LightCycler PCR: clinical and epidemiological aspects D. Johnsson, P. Mölling,

C. Steininger, M. Redlberger, W. Graninger, M. Kundi, T. Popow-Kraupp

Rapid detection of resistance in Mycobacterium tuberculosis: a review discussing molecular approaches D. García de Viedma Clinical Microbiology and.

Rapid and sensitive diagnosis of Toxoplasma gondii infections by PCR

Detection of small amounts of human adenoviruses in stools: comparison of a new immuno real-time PCR assay with classical tools S. Bonot, L. Ogorzaly,

Approach to diagnosis of infective endocarditis

Clinical significance of signal pattern of high-risk human papillomavirus using a novel fluorescence in situ hybridization assay in cervical cytology

C.-P. Kuo, C.-L. Wu, H.-T. Ho, C.G. Chen, S.-I. Liu, Y.-T. Lu

Herpes zoster in non-hospitalized children

Rapid Diagnosis of Influenza

E. Bronska, J. Kalmusova, O. Dzupova, V. Maresova, P. Kriz, J. Benes

J.-P. Van geertruyden Clinical Microbiology and Infection

Infectious causes of cancer: an evolving educational saga

A comparison of three real-time PCR assays for the confirmation of Neisseria gonorrhoeae following detection of N. gonorrhoeae using Roche COBAS AMPLICOR

Detection of Panton–Valentine leukocidin gene in Staphylococcus aureus by LightCycler PCR: clinical and epidemiological aspects D. Johnsson, P. Mölling,

The microarray technology: facts and controversies

G. Zhao, H. Zhai, Q. Yuan, S. Sun, T. Liu, L. Xie

Clinical characteristics, outcome and the role of viral load in nontransplant patients with Epstein--Barr viraemia T. Martelius, V.-J. Anttila Clinical.

Development of a real-time PCR targeting the yidC gene for the detection of Mycoplasma hominis and comparison with quantitative culture C. Férandon,

Real-time PCR assay-based strategy for differentiation between active Pneumocystis jirovecii pneumonia and colonization in immunocompromised patients

R. Cantón Clinical Microbiology and Infection

Performance of Focus ELISA tests for herpes simplex virus type 1 (HSV-1) and HSV-2 antibodies among women in ten diverse geographical locations R. Ashley-Morrow,

P. Balraj, G. Vestris, D. Raoult, P. Renesto

Louis Pasteur, from crystals of life to vaccination

Rapid detection of the recently emerged Bordetella pertussis strains with the ptxP3 pertussis toxin promoter allele by real-time PCR T. Kallonen, J.

Development of a real-time fluorescence resonance energy transfer PCR to identify the main pathogenic Campylobacter spp. A. Ménard, F. Dachet, V. Prouzet-Mauleon,

Molecular and diagnostic clinical virology in real time

Molecular and diagnostic clinical virology in real time

Loop-mediated isothermal amplification assay for the diagnosis of retinitis caused by herpes simplex virus-1 A.K. Reddy, P.K. Balne, R.K. Reddy, A. Mathai,

Vector control: a cornerstone in the malaria elimination campaign

Comparison of respiratory virus shedding by conventional and molecular testing methods in patients with haematological malignancy L. Richardson, J. Brite,

Visual detection of IS6110 of Mycobacterium tuberculosis in sputum samples using a test based on colloidal gold and latex beads P. Upadhyay, M. Hanif,

A comparison of three real-time PCR assays for the confirmation of Neisseria gonorrhoeae following detection of N. gonorrhoeae using Roche COBAS AMPLICOR

Detection of human metapneumovirus and respiratory syncytial virus by duplex real-time RT-PCR assay in comparison with direct fluorescent assay P. Jokela,

A novel method for the rapid and prospective identification of Beijing Mycobacterium tuberculosis strains by high-resolution melting analysis M. Alonso,

Performance of hepatitis C virus (HCV) direct-acting antivirals in clinical trials and daily practice J.E. Arends, P.A.M. Kracht, A.I.M. Hoepelman Clinical.

Factors associated with delay to acyclovir administration in 184 patients with herpes simplex virus encephalitis J. Poissy, M. Wolff, A. Dewilde, F.

Characterization of two novel gene cassettes, dfrA27 and aadA16, in a non-O1, non- O139 Vibrio cholerae isolate from China J. Sun, M. Zhou, Q. Wu, Y.

Rapid field detection assays for Bacillus anthracis, Brucella spp

Evaluation of a single-tube real-time PCR for detection and identification of 11 dermatophyte species in clinical material A.M.C. Bergmans, M. van der.

Metagenomics and probiotics

Detection of small amounts of human adenoviruses in stools: comparison of a new immuno real-time PCR assay with classical tools S. Bonot, L. Ogorzaly,

A novel fluorescence in situ hybridization test for rapid pathogen identification in positive blood cultures A. Makristathis, S. Riss, A.M. Hirschl

Evaluation of eight commercial tests for Mycoplasma pneumoniae antibodies in the absence of acute infection R. Nir-Paz, A. Michael-Gayego, M. Ron, C.

Real-time PCR assays compared to culture-based approaches for identification of aerobic bacteria in chronic wounds J.H. Melendez, Y.M. Frankel, A.T.

R. Slinger, I. Moldovan, N. Barrowman, F. Chan

A.P. Underwood, J. Green Clinical Microbiology and Infection

H. Leblebicioglu, C. Eroglu Clinical Microbiology and Infection

Diagnostic value of quantitative PCR for adenovirus detection in stool samples as compared with antigen detection and cell culture in haematopoietic stem.

An after-hours clinical liaison blood culture service—is it worth it?

Statin use and clinical outcomes among pneumonia patients

European guidelines for diagnosis and management of patients with suspected herpes simplex encephalitis Miles Denton, Kevin G Kerr, Fraser Lewis Clinical.

Real-time comparative monitoring of the A/H1N1 pandemic in France

K. Kaier, N.T. Mutters, U. Frank Clinical Microbiology and Infection

Multiplexed whole bacterial antigen microarray, a new format for the automation of serodiagnosis: the culture-negative endocarditis paradigm F. Gouriet,

The atypical pneumonias: clinical diagnosis and importance

Evaluation of diagnostic tests

Development and Comparison of a Rapid Isothermal Nucleic Acid Amplification Test for Typing of Herpes Simplex Virus Types 1 and 2 on a Portable Fluorescence.

Usefulness of a quantitative real-time PCR assay using serum samples to discriminate between inactive, serologically positive and active human brucellosis

Three years experience of real-time PCR for the diagnosis of Q fever

The future of diagnostic bacteriology

Presentation transcript:

Development and clinical evaluation of a novel fully automated qualitative PCR assay for the diagnosis of anogenital herpes simplex virus infection Steve Miller, Erik Samayoa, Linda Post, Carolyn Wright, Geoff McKinley, Michael Wood, Jesus Ching Diagnostic Microbiology and Infectious Disease Volume 80, Issue 2, Pages 102-106 (October 2014) DOI: 10.1016/j.diagmicrobio.2014.06.013 Copyright © 2014 Elsevier Inc. Terms and Conditions

Fig. 1 IDbox HSV 1/2 assay cassette showing reagent and sample reaction chambers. The sample is manually added and cassette placed in the instrument. The instrument automatically adds the internal control, performs proteinase K lysis, and binds magnetic silica beads. The beads are passed to wash chambers using magnetic bead transfer valves, and the purified nucleic acid is added to the PCR reaction chamber, which contains lyophilized primers, probes, and master mix. Real-time PCR proceeds on the instrument, and the signal and re-association curves are determined using fluorescent detectors. Diagnostic Microbiology and Infectious Disease 2014 80, 102-106DOI: (10.1016/j.diagmicrobio.2014.06.013) Copyright © 2014 Elsevier Inc. Terms and Conditions

Fig. 2 Receiver-operator curves showing comparison of IDbox results to HSV culture (A) and HSV PCR (B). Diagnostic Microbiology and Infectious Disease 2014 80, 102-106DOI: (10.1016/j.diagmicrobio.2014.06.013) Copyright © 2014 Elsevier Inc. Terms and Conditions

Fig. 3 Comparison of IDbox HSV 1/2 assay re-association peak results with real-time PCR viral load and PCR sequencing. Samples yielding positive sequencing results are shown as boxes, while samples yielding negative sequencing results are shown as diamonds. Diagnostic Microbiology and Infectious Disease 2014 80, 102-106DOI: (10.1016/j.diagmicrobio.2014.06.013) Copyright © 2014 Elsevier Inc. Terms and Conditions