Kalinat Anti wrinkle

An innovative and Powerful KALINAT A-W An innovative and Powerful ANTI AGE & ELASTICIZING Ingredient KALINAT A-W

Sodium salt of deoxyribonucleic acid (DNA) segments COMPOSITION COMPOSITION Sodium salt of deoxyribonucleic acid (DNA) segments KALINAT A-W INCI NAME Sodium DNA PURITY Powder pure @100% PRODUCTION SCHEME: DNA Extraction (biotechnologies) Purification - stabilization Buffering through sodium ions

In Vitro Test

FIBROBLAST STIMULATION % Fibroblast growth after 24 hours of exposure to different amounts of Kalinat KALINAT A-W 110 % SUGGESTED USAGE % 0,1 ÷ 0,25 1,00 % 0,50 % 0,25 % 0,13 % 0,06 % % Fibroblast growth 100 % Control

1 – UVA PHOTO TOXICITY Vitality of irradiated fibroblasts Capacity to protect cells in UVA “stressed” culture KALINAT A-W UVA UVA DEcrease of the cellular vitality Fibroblasts + Kalinat INcrease of the cellular vitality, Fibroblasts

In Test Vivo

IN VIVO INSTRUMENTAL EVALUATION Emulsion Kalinat® @ 0,25% vs. placebo 20 subjects, Average age 49 8 weeks Two application par day Right & Left peri-ocular area KALINAT A-W

IN VIVO TESTS Moisture level (Corneometer C&K) Cutaneous thickness (Dermascan C Ver3) Elasticity (Cutometer C&K) Maximum Roughness (Skin replica) Skin replica pictures KALINAT A-W

1 – MOISTURE LEVEL + 5% + 0% % of increase of moisture levol 64 % 62 % KALINAT A-W 64 % 62 % % of increase of moisture levol 60 % Week # 0 Week # 8 Week # 0 Week # 8 with Kalinat AW with Placebo + 5% + 0%

2 – CUTANEUS THICKNESS + 8,7% vs + 2% KALINAT A-W 1,50 1,43 Week # 0 Week # 8 1,39 Cutaneus Thickness (mm) with Kalinat AW with Placebo + 8,7% vs + 2%

3 – ELASTICITY + 25,6% vs < 0% VERY HIGH KALINAT A-W 0,49 0,38 0,35 Week # 0 Week # 8 Elasticity with Kalinat AW with Placebo + 25,6% vs < 0%

4 – MAXIMUM ROUGHNESS - 4% vs - 1,9% KALINAT A-W 157,7 154,9 151,4 Max.Rugosity (µm) Week # 0 Week # 8 with Kalinat AW with Placebo - 4% vs - 1,9%

5 – SKIN REPLICA PICTURES BEFORE AFTER KALINAT A-W BEFORE AFTER Week # 0 Week # 8

In Vitro test Very Important fibroblast stimulation CONCLUSION Very Important fibroblast stimulation No cytotoxic or photo-toxic effects Protective and regenerative action on the epidermis KALINAT A-W

In Vivo test + 5% + 9% Moisture level Cutaneous thickness + 25% CONCLUSION + 5% + 9% + 25% - 4% KALINAT A-W Moisture level Cutaneous thickness Elasticity Maximum Roughness

Ingredient that can be used in… THUS… a very powerful ANTI AGE & ELASTICIZING Ingredient that can be used in… KALINAT A-W

SUITABLE FOR Sun care products (sunscreen creams and milk, after sun) Anti-age & Anti-wrinkle face cream Sun care products (sunscreen creams and milk, after sun) Baby products (protective, soothing) Treatments for dry skins Treatment for damaged skins (i.e. stretch marks) KALINAT A-W

Via Pastore 1 – 25082 (Brescia) Italy Thank you KALINAT A-W Via Pastore 1 – 25082 (Brescia) Italy Tel. +39 0302693532 - Fax. +39 0302193581 www.kalichem.it sales@kalichem.it