From: Transient Hyperglycemia Affects the Extent of Ischemia-Reperfusion-induced Renal Injury in Rats Anesthes. 2008;108(3):402-414. doi:10.1097/ALN.0b013e318164cff8.

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From: Transient Hyperglycemia Affects the Extent of Ischemia-Reperfusion-induced Renal Injury in Rats Anesthes. 2008;108(3):402-414. doi:10.1097/ALN.0b013e318164cff8 Figure Legend: Fig. 3. Hyperglycemia down-regulated the expression of vascular endothelial growth factor (VEGF). ( A ) Quantitative messenger RNA (mRNA) expression of VEGF as assessed by TaqMan quantitative real-time polymerase chain reaction in renal tissue 24 h after reperfusion. Values were normalized to β-actin and are expressed as a ratio of sham ± SD, with n = 4 in each group. VEGF mRNA levels did not change in the saline group after ischemia-reperfusion compared with sham but were significantly decreased in the PI dextrose group. ( B ) Representative immunoblot for VEGF in kidneys after ischemia-reperfusion along with densitometric analysis. VEGF protein was measured by Western blot in 50 μg of kidney homogenates. Densitometric values were normalized to actin and are expressed as a ratio of sham ± SD, with n = 4 each in the sham and saline control groups and n = 6 each in the PI dextrose and PR dextrose groups. VEGF protein levels did not change in the saline group after ischemia-reperfusion compared with sham but were significantly decreased in the PI dextrose group. * P < 0.05 versus sham. $ P < 0.05 versus PR dextrose group. # P < 0.05 versus saline. VEGF expression in the PR dextrose group was similar to that in the saline group. PI dextrose = hyperglycemia lasts through the duration of ischemia; PR dextrose = hyperglycemia begins 2 h after initiation of reperfusion. Date of download: 10/16/2017 Copyright © 2017 American Society of Anesthesiologists. All rights reserved.