Plant Lipids Peter Dörmann

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Presentation transcript:

Plant Lipids Peter Dörmann Institute of Molecular Physiology and Biotechnology (IMBIO) Bonn: since February 2008 MPI Golm: until January 2008

Lipids are essential for photosynthesis DGDG (%) 15.0 1.5 WT dgd1

Storage lipids in seeds: human nutrition and biofuel production Plant Oil Salade oil Oil body in the cytosol of plant cells Biodiesel

Plant lipids and plant microbe interactions N2 -> NH4+ Lotus japonicus Lotus nodules infection Rhizobium thread Plant galactolipids are required for nodulation Mesorhizobium loti

Galactolipids replaces phospholipids during phosphate limitation R-P- nucleic acids phosphoproteins sugar-phosphates Phospholipids DGDG - P Pi deficiency Pi - P

The lipid response to abiotic stress: phosphate deprivation Arabidopsis thaliana Lotus japoncius Total Lipid Extract Lipid measurements: Targeted approaches for defined lipid classes Non-targeted approaches for novel and unknown lipids

Targeted lipid analysis Phosphatidylcholine Phosphatidylethanolamine Phosphatidylglycerol Phosphatidylserine Phosphatidylinositol Phosphatidic acid Monogalactosyldiacylglycerol Diagalactosyldiacylglycerol Sufolipid Ceramide Glucosylceramide Free Sterol, Sterol ester Sterol glucoside Acylated sterol glucoside Diacylglycerol Triacylglycerol Wax ester Tocopherol, Plastoquinol, Phylloquinol Targeted lipid analysis Lipid LC-MS (Q-TOF) GC-MS In total: More than 300 molecular species

Nont-targeted screening for stress-dependent lipids Example: TOF lipid mass spectra of roots versus nodules Roots Ca. 3000 lipid peaks each Nodules 21 masses > 50 x increased + 21 masses > 20 x increased + 35 masses > 10 x increased + 64 masses > 5 x increased + 289 masses > 2 x increased Characterization and identification of compounds by fragmentation

Aims: Record lipid changes during phoshpate deprivation Time courses: early (lipid signalling molecules), late changes Changes in lipid intermediates and byproducts: diacylglycerol, triacylglycerol Arabidopsis lipid mutants Include lipids not been previously measured (Glycosylinositolphosphoceramides) Plant species: Arabidopsis, Lotus japonicus (legume), barley (monocot) -OH

Outloock Abiotic stresses: cold stress, freezing stress Senescence Additional plant species...