In Situ Detection of Hepatitis C Virus RNA in Salivary Glands

Slides:

Advertisements

Similar presentations

Functional Analysis of the Neurofibromatosis Type 2 Protein by Means of Disease- Causing Point Mutations Renee P. Stokowski, David R. Cox The American.

Advertisements

Gene Preference in Maple Syrup Urine Disease Mary M. Nellis, Dean J. Danner The American Journal of Human Genetics Volume 68, Issue 1, Pages (January.

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.

Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,

Telomerase Activity and Expression of Telomerase RNA Component and Telomerase Catalytic Subunit Gene in Cervical Cancer Kenji Nakano, Elizabeth Watney,

Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.

Enterococcus faecalis Induces Inflammatory Bowel Disease in Interleukin-10 Knockout Mice Edward Balish, Thomas Warner The American Journal of Pathology.

Reduced Tumor Necrosis Factor-α and Transforming Growth Factor-β1 Expression in the Lungs of Inbred Mice that Fail to Develop Fibroproliferative Lesions.

Volume 86, Issue 3, Pages (September 2014)

Collagenase-3 (MMP-13) is Expressed by Tumor Cells in Invasive Vulvar Squamous Cell Carcinomas Nina Johansson, Maarit Vaalamo, Seija Grénman, Sakari.

Mutation Screening of EXT1 and EXT2 by Denaturing High-Performance Liquid Chromatography, Direct Sequencing Analysis, Fluorescence in Situ Hybridization,

Figure 2 In situ hybridization for rhinovirus type 14 (rhinovirus-14) in maxillary sinus biopsy specimens. A, Detection of rhinovirus-14, antisense probe,

Automated Quantitative RNA in Situ Hybridization for Resolution of Equivocal and Heterogeneous ERBB2 (HER2) Status in Invasive Breast Carcinoma Zhen.

Human rhinovirus in bronchial epithelium of infants with recurrent respiratory symptoms Kristiina Malmström, MD, PhD, Anne Pitkäranta, MD, PhD, Olli Carpen,

The CXC Receptor 2 Is Overexpressed in Psoriatic Epidermis

Detection of TMPRSS2-ETS Fusions by a Multiprobe Fluorescence in Situ Hybridization Assay for the Early Diagnosis of Prostate Cancer Qi-Peng Sun, Liao-Yuan.

Quiz Page June 2007 American Journal of Kidney Diseases

Statistical Considerations for Immunohistochemistry Panel Development after Gene Expression Profiling of Human Cancers Rebecca A. Betensky, Catherine.

Molecular Diagnosis of Epstein-Barr Virus-Related Diseases

Chromogenic In Situ Hybridization for α6β4 Integrin in Breast Cancer

Detection of TMPRSS2-ETS Fusions by a Multiprobe Fluorescence in Situ Hybridization Assay for the Early Diagnosis of Prostate Cancer Qi-Peng Sun, Liao-Yuan.

RNAscope The Journal of Molecular Diagnostics

H. Lepidi, F. Gouriet, D. Raoult Clinical Microbiology and Infection

Laboratory Guidelines for Detection, Interpretation, and Reporting of Maternal Cell Contamination in Prenatal Analyses Narasimhan Nagan, Nicole E. Faulkner,

Molecular Testing Guideline for Selection of Lung Cancer Patients for EGFR and ALK Tyrosine Kinase Inhibitors Neal I. Lindeman, Philip T. Cagle, Mary.

Volume 54, Issue 6, Pages (January 1998)

Detection of Cytomegalovirus in Whole Blood Using Three Different Real-Time PCR Chemistries Erica Vincent, Zhengming Gu, Markus Morgenstern, Candace.

Automated Quantitative RNA in Situ Hybridization for Resolution of Equivocal and Heterogeneous ERBB2 (HER2) Status in Invasive Breast Carcinoma Zhen.

Joanna Wang, Chetan Bettegowda The Journal of Molecular Diagnostics

Kei Kuroda, Allen Sapadin, Toru Shoji, Raul Fleischmajer, Mark Lebwohl

Increased Sensitivity of the Roche COBAS AMPLICOR HCV Test, Version 2

The Development of a Multitarget, Multicolor Fluorescence in Situ Hybridization Assay for the Detection of Urothelial Carcinoma in Urine Irina A. Sokolova,

Volume 130, Issue 4, Pages (April 2006)

Hepatitis C core protein – The “core” of immune deception?

William L. Gerald, M.D., Ph.D, 1954–2008

In situ localization and quantification of seventy-two – kilodalton type IV collagenase in aneurysmal, occlusive, and normal aorta William D. McMillan,

Masamoto Murakami, Takaaki Ohtake, Robert A. Dorschner, Richard L

Diagnosis of BK Viral Nephropathy in the Renal Allograft Biopsy

Absence of the Caveolin-1 P132L Mutation in Cancers of the Breast and Other Organs Shinya Koike, Yasuhiro Kodera, Akimasa Nakao, Hiroji Iwata, Yasushi.

Development of a Quantitative Real-Time Polymerase Chain Reaction Assay for the Detection of the JAK2 V617F Mutation Elizabeth C. Wolstencroft, Katy.

The Molecular Pathology of Primary Immunodeficiencies

Human Papillomavirus Gene Expression in Cutaneous Squamous Cell Carcinomas from Immunosuppressed and Immunocompetent Individuals Karin J. Purdie, Thirunavukarasu.

Cutaneous Lymphoma Associated With Epstein-Barr Virus Infection in 2 Patients Treated With Methotrexate Anne Tournadre, MD, Michel D'Incan, MD, PhD,

Percentage of Hepatitis C Virus-Infected Hepatocytes Is a Better Predictor of Response Than Serum Viremia Levels Elena Rodríguez-Iñigo, Juan Manuel López-Alcorocho,

Magnus Åbrink, Eric Larsson, Anders Gobl, Lars Hellman

Pityriasis Rosea is Associated with Systemic Active Infection with Both Human Herpesvirus-7 and Human Herpesvirus-6 Takahiro Watanabe, Tatsuyoshi Kawamura,

Presence of Epstein–Barr Virus in Langerhans Cells of CTCL Lesions

The CXCR3 Activating Chemokines IP-10, Mig, and IP-9 are Expressed in Allergic but not in Irritant Patch Test Reactions Jacoba Flier, Dick M. Boorsma,

The Detection of t(14;18) in Archival Lymph Nodes

Prevalence and Treatment of Hepatitis C Virus Genotypes 4, 5, and 6

In situ non-radioactive detection of nuclear factors in paraffin sections by Southwestern histochemistry Miguel Angel Hernández-Presa, Carmen Gómez-Guerrero,

Erratum The American Journal of Human Genetics

Role of Cytotoxic T Cells in Chronic Alopecia Areata

Diagnosis of BK Viral Nephropathy in the Renal Allograft Biopsy

Enhanced Expression of Human Metalloelastase (MMP-12) in Cutaneous Granulomas and Macrophage Migration Maarit Vaalamo, Arja-Leena Kariniemi, Ulpu Saarialho-Kere

Table of contents The Journal for Nurse Practitioners

Society for Investigative Dermatology 2010 Meeting Minutes

Multiple Epidermal Connexins are Expressed in Different Keratinocyte Subpopulations Including Connexin 31 Wei-Li Di, Elizabeth L. Rugg, Irene M. Leigh,

Serpins in the Human Hair Follicle

Synthesis of Collagen I in Collagenous Sprue

Circulating Tumor Cells and Detection of the Melanoma-Associated Antigen HMW-MAA in the Serum of Melanoma Patients Anja Ulmer, Gerhard Fierlbeck Journal.

Clinical Gastroenterology and Hepatology

Quiz Page June 2009 American Journal of Kidney Diseases

Volume 65, Issue 6, Pages (June 2004)

Quiz Page January 2010 American Journal of Kidney Diseases

Epidermal Interferon-γ Inducible Protein-10 (IP-10) and Monokine Induced by γ- Interferon (Mig) but not IL-8 mRNA Expression is Associated with Epidermotropism.

Optimized Allele-Specific Real-Time PCR Assays for the Detection of Common Mutations in KRAS and BRAF Alois H. Lang, Heinz Drexel, Simone Geller-Rhomberg,

Volume 120, Issue 4, Pages (March 2001)

News & Notes Journal of Allergy and Clinical Immunology

Chitinases and chitinase-like proteins in TH2 inflammation and asthma

Presentation transcript:

In Situ Detection of Hepatitis C Virus RNA in Salivary Glands Juan José Arrieta, Elena Rodríguez-Iñigo, Nuria Ortiz-Movilla, Javier Bartolomé, Margarita Pardo, Félix Manzarbeitia, Horacio Oliva, David Mariscal Macías, Vicente Carreño The American Journal of Pathology Volume 158, Issue 1, Pages 259-264 (January 2001) DOI: 10.1016/S0002-9440(10)63964-8 Copyright © 2001 American Society for Investigative Pathology Terms and Conditions

Figure 1 In situ hybridization in salivary gland biopsies from an anti-HCV-positive (a) and -negative (b) patients. Original magnification, ×400. Intracellular localization of the hybridization signal (c). Original magnification, ×1,000. Counterstained with safranine. The American Journal of Pathology 2001 158, 259-264DOI: (10.1016/S0002-9440(10)63964-8) Copyright © 2001 American Society for Investigative Pathology Terms and Conditions

Figure 2 In situ hybridization of serial sections of the same salivary gland biopsy with (a) and without (b) predigestion with RNase and hybridized in the presence (c) or in the absence (d) of an excess of unlabeled probe. Original magnification, ×1,000. Counterstained with safranine. The American Journal of Pathology 2001 158, 259-264DOI: (10.1016/S0002-9440(10)63964-8) Copyright © 2001 American Society for Investigative Pathology Terms and Conditions

Figure 3 In situ detection of HCV-RNA of sense (a) and antisense (b) polarity. Original magnification, ×400. Counterstained with safranine. The American Journal of Pathology 2001 158, 259-264DOI: (10.1016/S0002-9440(10)63964-8) Copyright © 2001 American Society for Investigative Pathology Terms and Conditions

Figure 4 Dot blot hybridization of serial dilutions of synthetic HCV-RNA of genomic and antigenomic polarity with the HCV antisense (a) and sense (b) probes. The American Journal of Pathology 2001 158, 259-264DOI: (10.1016/S0002-9440(10)63964-8) Copyright © 2001 American Society for Investigative Pathology Terms and Conditions

Figure 5 Immunological detection of HCV core antigen in salivary gland biopsies from a HCV-RNA-positive (a) and -negative (b) patients. Arrows show the positive acini for the HCV core protein. The American Journal of Pathology 2001 158, 259-264DOI: (10.1016/S0002-9440(10)63964-8) Copyright © 2001 American Society for Investigative Pathology Terms and Conditions