Antimicrobial activity of a UV-stable bacteriocin-like inhibitory substance (BLIS) produced by Enterococcus faecium strain DSH20 against vancomycin-resistant.

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Antimicrobial activity of a UV-stable bacteriocin-like inhibitory substance (BLIS) produced by Enterococcus faecium strain DSH20 against vancomycin-resistant Enterococcus (VRE) strains  Dariush Shokri, Saeideh Zaghian, Fatemeh Khodabakhsh, Hossein Fazeli, Sina Mobasherizadeh, Behrooz Ataei  Journal of Microbiology, Immunology and Infection  Volume 47, Issue 5, Pages 371-376 (October 2014) DOI: 10.1016/j.jmii.2013.05.004 Copyright © 2013 Terms and Conditions

Figure 1 Growth curve and bacteriocin-like inhibitory substance (BLIS) production of Enterococcus faecium strain DSH20 determined at 30°C. Journal of Microbiology, Immunology and Infection 2014 47, 371-376DOI: (10.1016/j.jmii.2013.05.004) Copyright © 2013 Terms and Conditions

Figure 2 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of partially purified bacteriocin-like inhibitory substance (BLIS) from Enterococcus faecium strain DSH20 after staining with Coomassie Brilliant Blue showed the molecular weight of approximately 35kDa. Line 1 shows a molecular mass marker (in kDa) and lines 2 and 3 show a molecular mass marker of purified BLIS with two repeats. Journal of Microbiology, Immunology and Infection 2014 47, 371-376DOI: (10.1016/j.jmii.2013.05.004) Copyright © 2013 Terms and Conditions

Figure 3 The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of bacteriocin-like inhibitory substance (BLIS) overlaying with Enterococcus faecalis ATCC 29212 as the sensitive indicator organism showed a clear inhibition zone after 24 hours at 30°C. Journal of Microbiology, Immunology and Infection 2014 47, 371-376DOI: (10.1016/j.jmii.2013.05.004) Copyright © 2013 Terms and Conditions