Impact of polymorphism rs7041 and rs4588 of Vitamin D Binding Protein on the extent of coronary artery disease V. Daffara, M. Verdoia, R. Rolla, M. Nardin,

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Impact of polymorphism rs7041 and rs4588 of Vitamin D Binding Protein on the extent of coronary artery disease V. Daffara, M. Verdoia, R. Rolla, M. Nardin, P. Marino, G. Bellomo, A. Carriero, G. De Luca Nutrition, Metabolism and Cardiovascular Diseases Volume 27, Issue 9, Pages 775-783 (September 2017) DOI: 10.1016/j.numecd.2017.06.002 Copyright © 2017 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University Terms and Conditions

Figure 1 Electrophoresis on High Resolution agarose gel after restriction of Polymerase Chain Reaction product with the enzymes: HaeIII (Fig. 1A), showing a double electrophoretic band for homozygotes GG patients or the three bands for TG heterozygotes for rs7041 and StyI (Fig. 1B) showing a double electrophoretic band for AA patients or the three bands for AC heterozygotes of rs4588 polymorphism. Nutrition, Metabolism and Cardiovascular Diseases 2017 27, 775-783DOI: (10.1016/j.numecd.2017.06.002) Copyright © 2017 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University Terms and Conditions

Figure 2 Polymorphisms rs7041(A) and rs4588 (B) and prevalence of severe deficiency of 25-hydroxyvitamin D. Nutrition, Metabolism and Cardiovascular Diseases 2017 27, 775-783DOI: (10.1016/j.numecd.2017.06.002) Copyright © 2017 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University Terms and Conditions

Figure 3 Polymorphisms rs7041 (A) and rs4588 (B) and prevalence of coronary artery disease (CAD). Nutrition, Metabolism and Cardiovascular Diseases 2017 27, 775-783DOI: (10.1016/j.numecd.2017.06.002) Copyright © 2017 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University Terms and Conditions

Figure 4 Polymorphisms rs7041 (A) and rs4588 (B) and prevalence of severe CAD. Nutrition, Metabolism and Cardiovascular Diseases 2017 27, 775-783DOI: (10.1016/j.numecd.2017.06.002) Copyright © 2017 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University Terms and Conditions