Figure 2. Raman bands from a representative line map across valve of Sypharochiton sinclairi. The lack of protein signals at 1110–1600 cm<sup>−1</sup>

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Figure 2. Raman bands from a representative line map across valve of Sypharochiton sinclairi. The lack of protein signals at 1110–1600 cm<sup>−1</sup> from the tegmentum, and of pigment signals at 1117 and 1499 cm<sup>−1</sup> from the articulamentum, were observed in all analysed species. The inset shows location of typical line maps on cross section of a valve; each grey dot is the location of a spectrum taken by the Raman microscope. Raman intensity is measured in arbitrary units (a.u.). Scale bar = 1 mm. From: First record of carotenoid pigments and indications of unusual shell structure in chiton valves J Molluscan Stud. Published online August 22, 2017.1-5 doi:10.1093/mollus/eyx033 J Molluscan Stud | © The Author 2017. Published by Oxford University Press on behalf of The Malacological Society of London, all rights reserved. For Permissions, please email: journals.permissions@oup.com

Figure 1. Raman spectra from the tegmentum and articulamentum of Leptochiton inquinatus, Onithochiton neglectus and Sypharochiton sinclairi. Bands at 704 and 1085 cm<sup>−1</sup> indicate aragonite, peaks at 1099–1117 and 1486–1499 cm<sup>−1</sup> indicate C=C and C–C bonds of carotenoids. Raman intensity is measured in arbitrary units (au). From: First record of carotenoid pigments and indications of unusual shell structure in chiton valves J Molluscan Stud. Published online August 22, 2017.1-5 doi:10.1093/mollus/eyx033 J Molluscan Stud | © The Author 2017. Published by Oxford University Press on behalf of The Malacological Society of London, all rights reserved. For Permissions, please email: journals.permissions@oup.com

Figure 3. Raman spectra of a decalcified Sypharochiton sinclairi valve and of α-chitin. The major peaks of α-chitin do not appear in the spectrum from S. sinclairi. Raman intensity is measured in arbitrary units (au). From: First record of carotenoid pigments and indications of unusual shell structure in chiton valves J Molluscan Stud. Published online August 22, 2017.1-5 doi:10.1093/mollus/eyx033 J Molluscan Stud | © The Author 2017. Published by Oxford University Press on behalf of The Malacological Society of London, all rights reserved. For Permissions, please email: journals.permissions@oup.com